Restriction map for pF1KSDA1994
 (Linear) (Six-base) MAPSORT of: KIAA1994.seq  Check: 7127  from: 1  to: 3219



KIAA1994.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     864    2307    3219 
   Size:        864    1443     912

  Fragments arranged by size: 

                1443     912     864
 
AccI GT'mk_AC

Cuts at:      0    1602    3219 
   Size:       1602    1617
 
Acc65I G'GTAC_C

Cuts at:      0     133    3219 
   Size:        133    3086
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1669    1748    1943    2237    3100    3219 
   Size:        175    1494      79     195     294     863     119

  Fragments arranged by size: 

                1494     863     294     195     175     119      79
 
AfeI AGC'GCT

Cuts at:      0      54    3219 
   Size:         54    3165
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2471    3219 
   Size:       2471     748
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2161    2845    3219 
   Size:       2161     684     374

  Fragments arranged by size: 

                2161     684     374
 
ApoI r'AATT_y

Cuts at:      0     912    2646    3048    3219 
   Size:        912    1734     402     171

  Fragments arranged by size: 

                1734     912     402     171
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     125     158    3219 
   Size:        125      33    3061

  Fragments arranged by size: 

                3061     125      33
 
BanI G'GyrC_C

Cuts at:      0     133     686    1860    3166    3219 
   Size:        133     553    1174    1306      53

  Fragments arranged by size: 

                1306    1174     553     133      53
 
BanII G_rGCy'C

Cuts at:      0     503    1253    1473    2756    2914    3205    3219 
   Size:        503     750     220    1283     158     291      14

  Fragments arranged by size: 

                1283     750     503     291     220     158      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1675    3088    3219 
   Size:       1675    1413     131

  Fragments arranged by size: 

                1675    1413     131
 
BbvCI CC'TCA_GC

Cuts at:      0     590    3219 
   Size:        590    2629
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1738    2083    3219 
   Size:       1738     345    1136

  Fragments arranged by size: 

                1738    1136     345
 
BclI T'GATC_A

Cuts at:      0     629    3219 
   Size:        629    2590
 
BglII A'GATC_T

Cuts at:      0     359     441    1628    3219 
   Size:        359      82    1187    1591

  Fragments arranged by size: 

                1591    1187     359      82
 
BlpI GC'TnA_GC

Cuts at:      0     272    2060    3219 
   Size:        272    1788    1159

  Fragments arranged by size: 

                1788    1159     272
 
Bme1580I G_kGCm'C

Cuts at:      0     689    1863    3169    3219 
   Size:        689    1174    1306      50

  Fragments arranged by size: 

                1306    1174     689      50
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     763    1175    2009    2318    2679    3105    3219 
   Size:        763     412     834     309     361     426     114

  Fragments arranged by size: 

                 834     763     426     412     361     309     114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1634    2931    3219 
   Size:       1634    1297     288

  Fragments arranged by size: 

                1634    1297     288
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1079    1820    3079    3219 
   Size:       1079     741    1259     140

  Fragments arranged by size: 

                1259    1079     741     140
 
Bpu10I CC'TnA_GC

Cuts at:      0     590    2708    3098    3204    3219 
   Size:        590    2118     390     106      15

  Fragments arranged by size: 

                2118     590     390     106      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     747    1110    1637    2414    3219 
   Size:        747     363     527     777     805

  Fragments arranged by size: 

                 805     777     747     527     363
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517    3219 
   Size:        517    2702
 
BsaHI Gr'CG_yC

Cuts at:      0     140    3219 
   Size:        140    3079
 
BsaWI w'CCGG_w

Cuts at:      0    2005    3219 
   Size:       2005    1214
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     641     671    1517    1547    2325    2355    2382    2412 
   Size:        641      30     846      30     778      30      27      30

Cuts at:   2412    3219 
   Size:        807

  Fragments arranged by size: 

                 846     807     778     641      30      30      30      30
                  27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     504    1823    2264    2904    3219 
   Size:        504    1319     441     640     315

  Fragments arranged by size: 

                1319     640     504     441     315
 
BseYI C'CCAG_C

Cuts at:      0    1808    2103    2402    3053    3122    3155    3185    3219 
   Size:       1808     295     299     651      69      33      30      34

  Fragments arranged by size: 

                1808     651     299     295      69      34      33      30
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1507    2107    2245    2765    3219 
   Size:       1507     600     138     520     454

  Fragments arranged by size: 

                1507     600     520     454     138
 
BsiEI CG_ry'CG

Cuts at:      0      32    3219 
   Size:         32    3187
 
BsiHKAI G_wGCw'C

Cuts at:      0     885    1393    2274    2343    2914    3219 
   Size:        885     508     881      69     571     305

  Fragments arranged by size: 

                 885     881     571     508     305      69
 
BsmI GAATG_Cn'

Cuts at:      0     645    2534    3219 
   Size:        645    1889     685

  Fragments arranged by size: 

                1889     685     645
 
Bsp1286I G_dGCh'C

Cuts at:      0     503     689     885    1253    1393    1473    1863    2274 
   Size:        503     186     196     368     140      80     390     411

Cuts at:   2274    2343    2756    2914    3169    3205    3219 
   Size:         69     413     158     255      36      14

  Fragments arranged by size: 

                 503     413     411     390     368     255     196     186
                 158     140      80      69      36      14
 
BspEI T'CCGG_A

Cuts at:      0    2005    3219 
   Size:       2005    1214
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121     864    1317    2121    2307    3219 
   Size:        121     743     453     804     186     912

  Fragments arranged by size: 

                 912     804     743     453     186     121
 
BsrDI GCAATG_nn'

Cuts at:      0      83    1110    2003    3219 
   Size:         83    1027     893    1216

  Fragments arranged by size: 

                1216    1027     893      83
 
BsrFI r'CCGG_y

Cuts at:      0     136    3219 
   Size:        136    3083
 
BsrGI T'GTAC_A

Cuts at:      0    1968    3219 
   Size:       1968    1251
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2113    2161    2845    3219 
   Size:       2113      48     684     374

  Fragments arranged by size: 

                2113     684     374      48
 
BstEII G'GTnAC_C

Cuts at:      0     703    3219 
   Size:        703    2516
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2137    3219 
   Size:       2137    1082
 
BstYI r'GATC_y

Cuts at:      0     359     441     458    1628    2964    3219 
   Size:        359      82      17    1170    1336     255

  Fragments arranged by size: 

                1336    1170     359     255      82      17
 
BstZ17I GTA'TAC

Cuts at:      0    1603    3219 
   Size:       1603    1616
 
BtgI C'CryG_G

Cuts at:      0      26    3219 
   Size:         26    3193
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1721    3179    3219 
   Size:       1721    1458      40

  Fragments arranged by size: 

                1721    1458      40
 
BtsI GCAGTG_nn'

Cuts at:      0    1143    2162    2228    3219 
   Size:       1143    1019      66     991

  Fragments arranged by size: 

                1143    1019     991      66
 
DraI TTT'AAA

Cuts at:      0    1257    3219 
   Size:       1257    1962
 
EaeI y'GGCC_r

Cuts at:      0      29     731    2139    3089    3219 
   Size:         29     702    1408     950     130

  Fragments arranged by size: 

                1408     950     702     130      29
 
EagI C'GGCC_G

Cuts at:      0      29    3219 
   Size:         29    3190
 
EarI CTCTTCn'nnn_

Cuts at:      0     453     515    3219 
   Size:        453      62    2704

  Fragments arranged by size: 

                2704     453      62
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     164     227     317    3219 
   Size:        164      63      90    2902

  Fragments arranged by size: 

                2902     164      90      63
 
EcoICRI GAG'CTC

Cuts at:      0    2912    3219 
   Size:       2912     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1079    1669    1748    1820    1943    2237    3079 
   Size:        175     904     590      79      72     123     294     842

Cuts at:   3079    3100    3219 
   Size:         21     119

  Fragments arranged by size: 

                 904     842     590     294     175     123     119      79
                  72      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     265    3219 
   Size:        265    2954
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3012    3219 
   Size:       3012     207
 
HaeII r_GCGC'y

Cuts at:      0      56     113    3219 
   Size:         56      57    3106

  Fragments arranged by size: 

                3106      57      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1935    1967    2304    2336    2896    2928    3219 
   Size:       1935      32     337      32     560      32     291

  Fragments arranged by size: 

                1935     560     337     291      32      32      32
 
HincII GTy'rAC

Cuts at:      0     948    1206    2390    2620    3219 
   Size:        948     258    1184     230     599

  Fragments arranged by size: 

                1184     948     599     258     230
 
HpaI GTT'AAC

Cuts at:      0     948    1206    2390    3219 
   Size:        948     258    1184     829

  Fragments arranged by size: 

                1184     948     829     258
 
KpnI G_GTAC'C

Cuts at:      0     137    3219 
   Size:        137    3082
 
MfeI C'AATT_G

Cuts at:      0    2359    2714    3219 
   Size:       2359     355     505

  Fragments arranged by size: 

                2359     505     355
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     720    1575    3219 
   Size:        720     855    1644

  Fragments arranged by size: 

                1644     855     720
 
MscI TGG'CCA

Cuts at:      0     733    2141    3091    3219 
   Size:        733    1408     950     128

  Fragments arranged by size: 

                1408     950     733     128
 
MspA1I CmG'CkG

Cuts at:      0      28      40      98     276    1153    2079    2749    2877 
   Size:         28      12      58     178     877     926     670     128

Cuts at:   2877    3057    3126    3219 
   Size:        180      69      93

  Fragments arranged by size: 

                 926     877     670     180     178     128      93      69
                  58      28      12
 
NotI GC'GGCC_GC

Cuts at:      0      29    3219 
   Size:         29    3190
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1557    3219 
   Size:       1557    1662
 
PfoI T'CCnGG_A

Cuts at:      0     539    3219 
   Size:        539    2680
 
PpuMI rG'GwC_Cy

Cuts at:      0    3012    3219 
   Size:       3012     207
 
PstI C_TGCA'G

Cuts at:      0      45     210     281    1158    2025    2118    2223    2460 
   Size:         45     165      71     877     867      93     105     237

Cuts at:   2460    3131    3219 
   Size:        671      88

  Fragments arranged by size: 

                 877     867     671     237     165     105      93      88
                  71      45
 
PvuII CAG'CTG

Cuts at:      0     276    1153    2079    3057    3126    3219 
   Size:        276     877     926     978      69      93

  Fragments arranged by size: 

                 978     926     877     276      93      69
 
SacI G_AGCT'C

Cuts at:      0    2914    3219 
   Size:       2914     305
 
SacII CC_GC'GG

Cuts at:      0      29    3219 
   Size:         29    3190
 
SbfI CC_TGCA'GG

Cuts at:      0    2118    3219 
   Size:       2118    1101
 
ScaI AGT'ACT

Cuts at:      0     891    3219 
   Size:        891    2328
 
SfcI C'TryA_G

Cuts at:      0      41     206     277     893    1154    1313    2021    2114 
   Size:         41     165      71     616     261     159     708      93

Cuts at:   2114    2219    2456    3127    3219 
   Size:        105     237     671      92

  Fragments arranged by size: 

                 708     671     616     261     237     165     159     105
                  93      92      71      41
 
SmlI C'TyrA_G

Cuts at:      0     762    1089    1652    2429    3219 
   Size:        762     327     563     777     790

  Fragments arranged by size: 

                 790     777     762     563     327
 
SpeI A'CTAG_T

Cuts at:      0     928    1393    2335    3219 
   Size:        928     465     942     884

  Fragments arranged by size: 

                 942     928     884     465
 
SspI AAT'ATT

Cuts at:      0    1026    2193    3219 
   Size:       1026    1167    1026

  Fragments arranged by size: 

                1167    1026    1026
 
StyI C'CwwG_G

Cuts at:      0     996    1536    3219 
   Size:        996     540    1683

  Fragments arranged by size: 

                1683     996     540
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3169    3219 
   Size:       3169      50
 
TatI w'GTAC_w

Cuts at:      0     889     931    1015    1638    1968    3219 
   Size:        889      42      84     623     330    1251

  Fragments arranged by size: 

                1251     889     623     330      84      42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     641     673    2030    2057    2062    2089    3219 
   Size:        641      32    1357      27       5      27    1130

  Fragments arranged by size: 

                1357    1130     641      32      27      27       5
 
XbaI T'CTAG_A

Cuts at:      0     775    3219 
   Size:        775    2444
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     697    1794    3130    3219 
   Size:        697    1097    1336      89

  Fragments arranged by size: 

                1336    1097     697      89
 
XmnI GAAnn'nnTTC

Cuts at:      0    2439    2532    3219 
   Size:       2439      93     687

  Fragments arranged by size: 

                2439     687      93
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AlfI    AlwNI     ApoI
     BaeI     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I   HincII     HpaI     KpnI     MfeI     MmeI     MscI
   MspA1I     NotI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
    SacII     SbfI     ScaI     SfcI     SmlI     SpeI     SspI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BbeI     BcgI    BfrBI     BglI    BmgBI     BmtI    BsaAI    BsaBI
    BsiWI    BsmBI    BspHI    BsrBI   BssHII    BssSI    BstBI   Bsu36I
     ClaI    CspCI   DraIII     DrdI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     KasI     MluI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     SwaI  Tth111I     XhoI     XmaI     ZraI
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