Restriction map for pF1KSDA1972
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BfrI        BglI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HhaI        HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MluI        MroI        MvnI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        Tth111I     Van91I      
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          914  GTAGAC               GT^[AC][GT]AC
AciI          107  CCGC                 CCGC(-3/-1)
AspHI          73  GAGCAC               G[AT]GC[AT]^C
AvaI         1136  CTCGAG               C^[CT]CG[GA]G
BalI           45  TGGCCA               TGG^CCA
BanI          103  GGTGCC               G^G[CT][GA]CC
BciVI        1562  GTATCC               GTATCC(6/5)
BclI          351  TGATCA               T^GATCA
BcnI         1073  CCGGG                CC^[GC]GG
BglII         995  AGATCT               A^GATCT
BsaBI         346  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1666  GAGACC               GGTCTC(1/5)
BseRI        1032  CTCCTC               GAGGAG(10/8)
BsgI          197  GTGCAG               GTGCAG(16/14)
BsiHKAI        73  GAGCAC               G[AT]GC[AT]^C
BsoBI        1136  CTCGAG               C^[CT]CG[GA]G
BstAPI        640  GCAGGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI          808  GACCGGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           70  GAAGAG               CTCTTC(1/4)
EcoNI          42  CCTTGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          351  TGATCA               T^GATCA
HphI         1000  TCACC                GGTGA(8/7)
Ksp632I        70  GAAGAG               CTCTTC(1/4)
MaeII         858  ACGT                 A^CGT
MamI          346  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1612  CAATTG               C^AATTG
MluNI          45  TGGCCA               TGG^CCA
MscI           45  TGGCCA               TGG^CCA
MslI          720  CACAGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1612  CAATTG               C^AATTG
NaeI         1211  GCCGGC               GCC^GGC
NciI         1073  CCGGG                CC^[GC]GG
NcoI          116  CCATGG               C^CATGG
NgoMIV       1211  GCCGGC               G^CCGGC
PaeR7I       1136  CTCGAG               C^TCGAG
SapI           70  GAAGAGC              GCTCTTC(1/4)
SwaI          548  ATTTAAAT             ATTT^AAAT
XbaI         1699  TCTAGA               T^CTAGA
XhoI         1136  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI         1111  GGCGTC               G[GA]^CG[CT]C
             1535  GGCGTC              
AlwI          457  GATCC                GGATC(4/5)
              841  GATCC               
AvrII          90  CCTAGG               C^CTAGG
              615  CCTAGG              
BcgI          189  CGAGAACAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1567  CGAGAAAACTGC        
BlnI           90  CCTAGG               C^CTAGG
              615  CCTAGG              
BmyI           73  GAGCAC               G[AGT]GC[ACT]^C
              875  GTGCCC              
BsaHI        1111  GGCGTC               G[GA]^CG[CT]C
             1535  GGCGTC              
BsmAI        1139  GAGAC                GTCTC(1/5)
             1666  GAGAC               
Bsp1286I       73  GAGCAC               G[AGT]GC[ACT]^C
              875  GTGCCC              
BspHI         270  TCATGA               T^CATGA
             1158  TCATGA              
BspMI        1669  ACCTGC               ACCTGC(4/8)
              640  GCAGGT              
BsrDI         503  CATTGC               GCAATG(2/0)
              971  GCAATG              
BsrFI         809  ACCGGC               [GA]^CCGG[CT]
             1211  GCCGGC              
BstYI         456  AGATCC               [GA]^GATC[CT]
              995  AGATCT              
Cfr10I        809  ACCGGC               [GA]^CCGG[CT]
             1211  GCCGGC              
DsaI          116  CCATGG               C^C[GA][CT]GG
              229  CCACGG              
HgaI         1112  GCGTC                GACGC(5/10)
             1536  GCGTC               
Hin1I        1111  GGCGTC               G[GA]^CG[CT]C
             1535  GGCGTC              
HincII         60  GTTGAC               GT[CT]^[GA]AC
              166  GTTGAC              
HindII         60  GTTGAC               GT[CT]^[GA]AC
              166  GTTGAC              
MflI          456  AGATCC               [GA]^GATC[CT]
              995  AGATCT              
MwoI          640  GCAGGTCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              736  GCACTTATCGC         
PstI          608  CTGCAG               CTGCA^G
             1391  CTGCAG              
RcaI          270  TCATGA               T^CATGA
             1158  TCATGA              
TaqI           68  TCGA                 T^CGA
             1137  TCGA                
TfiI          793  GAATC                G^A[AT]TC
             1703  GAATC               
TthHB8I        68  TCGA                 T^CGA
             1137  TCGA                
XhoII         456  AGATCC               [GA]^GATC[CT]
              995  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwNI       ApoI        
AvaII       BbvI        BfaI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MspI        MvaI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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