Restriction map for pF1KSDA1972
 (Linear) (Six-base) MAPSORT of: KIAA1972.seq  Check: 1842  from: 1  to: 1728



KIAA1972.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AccI GT'mk_AC

Cuts at:      0     915    1728 
   Size:        915     813
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1012    1526    1728 
   Size:        721     291     514     202

  Fragments arranged by size: 

                 721     514     291     202
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1357    1728 
   Size:       1357     371
 
AlwNI CAG_nnn'CTG

Cuts at:      0      54     646     699    1728 
   Size:         54     592      53    1029

  Fragments arranged by size: 

                1029     592      54      53
 
ApoI r'AATT_y

Cuts at:      0     539    1147    1468    1728 
   Size:        539     608     321     260

  Fragments arranged by size: 

                 608     539     321     260
 
AvaI C'yCGr_G

Cuts at:      0    1136    1728 
   Size:       1136     592
 
AvrII C'CTAG_G

Cuts at:      0      90     615    1728 
   Size:         90     525    1113

  Fragments arranged by size: 

                1113     525      90
 
BanI G'GyrC_C

Cuts at:      0     103    1728 
   Size:        103    1625
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     178     212    1556    1590    1728 
   Size:        178      34    1344      34     138

  Fragments arranged by size: 

                1344     178     138      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1573    1728 
   Size:       1573     155
 
BclI T'GATC_A

Cuts at:      0     351    1728 
   Size:        351    1377
 
BglII A'GATC_T

Cuts at:      0     995    1728 
   Size:        995     733
 
Bme1580I G_kGCm'C

Cuts at:      0     879    1728 
   Size:        879     849
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     472     873    1728 
   Size:        472     401     855

  Fragments arranged by size: 

                 855     472     401
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     508    1684    1728 
   Size:        119     389    1176      44

  Fragments arranged by size: 

                1176     389     119      44
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561     963    1728 
   Size:        561     402     765

  Fragments arranged by size: 

                 765     561     402
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1660    1728 
   Size:       1660      68
 
BsaBI GATnn'nnATC

Cuts at:      0     350    1728 
   Size:        350    1378
 
BsaHI Gr'CG_yC

Cuts at:      0    1112    1536    1728 
   Size:       1112     424     192

  Fragments arranged by size: 

                1112     424     192
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1265    1295    1728 
   Size:       1265      30     433

  Fragments arranged by size: 

                1265     433      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1023    1728 
   Size:       1023     705
 
BseYI C'CCAG_C

Cuts at:      0    1127    1728 
   Size:       1127     601
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218    1728 
   Size:        218    1510
 
BsiHKAI G_wGCw'C

Cuts at:      0      77    1728 
   Size:         77    1651
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     879    1728 
   Size:         77     802     849

  Fragments arranged by size: 

                 849     802      77
 
BspHI T'CATG_A

Cuts at:      0     270    1158    1728 
   Size:        270     888     570

  Fragments arranged by size: 

                 888     570     270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     631    1678    1728 
   Size:        631    1047      50

  Fragments arranged by size: 

                1047     631      50
 
BsrDI GCAATG_nn'

Cuts at:      0     502     978    1728 
   Size:        502     476     750

  Fragments arranged by size: 

                 750     502     476
 
BsrFI r'CCGG_y

Cuts at:      0     809    1211    1728 
   Size:        809     402     517

  Fragments arranged by size: 

                 809     517     402
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     646    1728 
   Size:        646    1082
 
BstYI r'GATC_y

Cuts at:      0     456     995    1728 
   Size:        456     539     733

  Fragments arranged by size: 

                 733     539     456
 
BtgI C'CryG_G

Cuts at:      0     116     229    1728 
   Size:        116     113    1499

  Fragments arranged by size: 

                1499     116     113
 
BtsI GCAGTG_nn'

Cuts at:      0     206    1728 
   Size:        206    1522
 
DraI TTT'AAA

Cuts at:      0     551     905    1328    1728 
   Size:        551     354     423     400

  Fragments arranged by size: 

                 551     423     400     354
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     814    1728 
   Size:        814     914
 
EaeI y'GGCC_r

Cuts at:      0      45     244     626    1728 
   Size:         45     199     382    1102

  Fragments arranged by size: 

                1102     382     199      45
 
EarI CTCTTCn'nnn_

Cuts at:      0      65    1728 
   Size:         65    1663
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     508     721    1012    1526    1684    1728 
   Size:        119     389     213     291     514     158      44

  Fragments arranged by size: 

                 514     389     291     213     158     119      44
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46    1728 
   Size:         46    1682
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234     265     643    1728 
   Size:        234      31     378    1085

  Fragments arranged by size: 

                1085     378     234      31
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1377    1728 
   Size:       1377     351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     987    1018    1019    1050    1174    1206    1728 
   Size:        987      31       1      31     124      32     522

  Fragments arranged by size: 

                 987     522     124      32      31      31       1
 
HincII GTy'rAC

Cuts at:      0      62     168    1728 
   Size:         62     106    1560

  Fragments arranged by size: 

                1560     106      62
 
MfeI C'AATT_G

Cuts at:      0    1612    1728 
   Size:       1612     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     395    1728 
   Size:        293     102    1333

  Fragments arranged by size: 

                1333     293     102
 
MscI TGG'CCA

Cuts at:      0      47    1728 
   Size:         47    1681
 
MslI CAynn'nnrTG

Cuts at:      0     724    1728 
   Size:        724    1004
 
MspA1I CmG'CkG

Cuts at:      0     472     921    1218    1341    1504    1662    1728 
   Size:        472     449     297     123     163     158      66

  Fragments arranged by size: 

                 472     449     297     163     158     123      66
 
NaeI GCC'GGC

Cuts at:      0    1213    1728 
   Size:       1213     515
 
NcoI C'CATG_G

Cuts at:      0     116    1728 
   Size:        116    1612
 
NgoMIV G'CCGG_C

Cuts at:      0    1211    1728 
   Size:       1211     517
 
PpuMI rG'GwC_Cy

Cuts at:      0     234     265     643    1728 
   Size:        234      31     378    1085

  Fragments arranged by size: 

                1085     378     234      31
 
PsiI TTA'TAA

Cuts at:      0     478    1728 
   Size:        478    1250
 
PstI C_TGCA'G

Cuts at:      0     612    1395    1728 
   Size:        612     783     333

  Fragments arranged by size: 

                 783     612     333
 
PvuII CAG'CTG

Cuts at:      0     472     921    1218    1341    1504    1662    1728 
   Size:        472     449     297     123     163     158      66

  Fragments arranged by size: 

                 472     449     297     163     158     123      66
 
SanDI GG'GwC_CC

Cuts at:      0     234    1728 
   Size:        234    1494
 
SapI GCTCTTCn'nnn_

Cuts at:      0      65    1728 
   Size:         65    1663
 
SfcI C'TryA_G

Cuts at:      0     608     691    1391    1728 
   Size:        608      83     700     337

  Fragments arranged by size: 

                 700     608     337      83
 
SmlI C'TyrA_G

Cuts at:      0     576     942    1136    1728 
   Size:        576     366     194     592

  Fragments arranged by size: 

                 592     576     366     194
 
StyI C'CwwG_G

Cuts at:      0      42      90     116     250     277     615    1525    1728 
   Size:         42      48      26     134      27     338     910     203

  Fragments arranged by size: 

                 910     338     203     134      48      42      27      26
            
 
SwaI ATTT'AAAT

Cuts at:      0     551    1728 
   Size:        551    1177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     187    1271    1728 
   Size:        187    1084     457

  Fragments arranged by size: 

                1084     457     187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      87     119    1728 
   Size:         87      32    1609

  Fragments arranged by size: 

                1609      87      32
 
XbaI T'CTAG_A

Cuts at:      0    1699    1728 
   Size:       1699      29
 
XhoI C'TCGA_G

Cuts at:      0    1136    1728 
   Size:       1136     592
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     AvaI    AvrII     BanI
     BcgI    BciVI     BclI    BglII Bme1580I     BmrI     BpmI    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstYI     BtgI
     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
     FalI    Hin4I   HincII     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV    PpuMI     PsiI     PstI    PvuII    SanDI
     SapI     SfcI     SmlI     StyI     SwaI    TaqII     TstI     XbaI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
   DraIII     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI     NarI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     TatI  Tth111I     XcmI
     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com