Restriction map for pF1KSDA1912
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflIII      AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AvrII       
BalI        BamHI       BbeI        BcgI        BciVI       
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaI        BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoRI       EcoT14I     EcoT22I     FseI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NarI        NcoI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Van91I      XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         1222  GTCTAC               GT^[AC][GT]AC
AflII        1567  CTTAAG               C^TTAAG
AgeI          959  ACCGGT               A^CCGGT
AscI          170  GGCGCGCC             GG^CGCGCC
AspI          383  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         538  TGCGCA               TGC^GCA
BbrPI         922  CACGTG               CAC^GTG
BbsI          298  GAAGAC               GAAGAC(2/6)
BbvCI          56  CCTCAGC              CCTCAGC(-5/-2)
BclI         1110  TGATCA               T^GATCA
BfrI         1567  CTTAAG               C^TTAAG
BpuAI         298  GAAGAC               GAAGAC(2/6)
BsmFI         317  GGGAC                GGGAC(10/14)
BssHII        171  GCGCGC               G^CGCGC
BstAPI        545  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1202  GGTCACC              G^GT[ACGT]ACC
BstPI        1202  GGTCACC              G^GT[ACGT]ACC
BstYI         260  AGATCC               [GA]^GATC[CT]
EcoNI        1253  CCTATGTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1202  GGTCACC              G^GT[ACGT]ACC
EcoRV         277  GATATC               GAT^ATC
FbaI         1110  TGATCA               T^GATCA
FspI          538  TGCGCA               TGC^GCA
HaeII         757  AGCGCC               [GA]GCGC^[CT]
HinfI        1240  GAATC                G^A[ACGT]TC
MflI          260  AGATCC               [GA]^GATC[CT]
MseI         1568  TTAA                 T^TAA
MslI         1213  CACCGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           67  GCCGGC               GCC^GGC
NgoMIV         67  GCCGGC               G^CCGGC
NheI         1510  GCTAGC               G^CTAGC
PflFI         383  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         959  ACCGGT               A^CCGGT
PmaCI         922  CACGTG               CAC^GTG
PmlI          922  CACGTG               CAC^GTG
SgrAI          92  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI         1449  CCCGGG               CCC^GGG
SphI         1304  GCATGC               GCATG^C
TfiI         1240  GAATC                G^A[AT]TC
Tru9I        1568  TTAA                 T^TAA
Tsp509I      1617  AATT                 ^AATT
Tth111I       383  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          681  CCACGCGAGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         260  AGATCC               [GA]^GATC[CT]
XmaCI        1449  CCCGGG               C^CCGGG
XmaI         1449  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI         1558  GTAC                 GT^AC
             1644  GTAC                
AlwI          156  GATCC                GGATC(4/5)
              261  GATCC               
BsaAI         607  TACGTG               [CT]AC^GT[GA]
              922  CACGTG              
BsaWI         959  ACCGGT               [AT]^CCGG[AT]
             1214  ACCGGA              
BsgI          247  CTGCAC               GTGCAG(16/14)
              530  GTGCAG              
BsiEI          11  CGGCCG               CG[GA][CT]^CG
               65  CGGCCG              
BsmAI         381  GAGAC                GTCTC(1/5)
              366  GTCTC               
DsaI           77  CCGCGG               C^C[GA][CT]GG
              264  CCGCGG              
EaeI           11  CGGCCG               [CT]^GGCC[GA]
               65  CGGCCG              
EagI           11  CGGCCG               C^GGCCG
               65  CGGCCG              
EclXI          11  CGGCCG               C^GGCCG
               65  CGGCCG              
Eco52I         11  CGGCCG               C^GGCCG
               65  CGGCCG              
KspI           77  CCGCGG               CCGC^GG
              264  CCGCGG              
MaeII         608  ACGT                 A^CGT
              923  ACGT                
NspI         1304  GCATGC               [GA]CATG^[CT]
             1524  ACATGC              
RsaI         1558  GTAC                 GT^AC
             1644  GTAC                
SacI          490  GAGCTC               GAGCT^C
              499  GAGCTC              
SacII          77  CCGCGG               CCGC^GG
              264  CCGCGG              
TaqI            4  TCGA                 T^CGA
              560  TCGA                
Tsp45I        623  GTGAC                ^GT[GC]AC
             1203  GTCAC               
TthHB8I         4  TCGA                 T^CGA
              560  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TseI        TspRI       
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