Restriction map for pF1KSDA1912
 (Linear) (Six-base) MAPSORT of: KIAA1912.seq  Check: 6388  from: 1  to: 1659



KIAA1912.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     716    1659 
   Size:        716     943
 
AccI GT'mk_AC

Cuts at:      0    1223    1659 
   Size:       1223     436
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1000    1500    1659 
   Size:       1000     500     159

  Fragments arranged by size: 

                1000     500     159
 
AflII C'TTAA_G

Cuts at:      0    1567    1659 
   Size:       1567      92
 
AgeI A'CCGG_T

Cuts at:      0     959    1659 
   Size:        959     700
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1476    1508    1659 
   Size:       1476      32     151

  Fragments arranged by size: 

                1476     151      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     312     384     441     551     791    1477    1659 
   Size:        312      72      57     110     240     686     182

  Fragments arranged by size: 

                 686     312     240     182     110      72      57
 
AscI GG'CGCG_CC

Cuts at:      0     171    1659 
   Size:        171    1488
 
AvaI C'yCGr_G

Cuts at:      0     368     766    1449    1659 
   Size:        368     398     683     210

  Fragments arranged by size: 

                 683     398     368     210
 
BanI G'GyrC_C

Cuts at:      0     881    1066    1171    1415    1659 
   Size:        881     185     105     244     244

  Fragments arranged by size: 

                 881     244     244     185     105
 
BanII G_rGCy'C

Cuts at:      0     494     503     940    1006    1108    1389    1659 
   Size:        494       9     437      66     102     281     270

  Fragments arranged by size: 

                 494     437     281     270     102      66       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     305    1659 
   Size:        305    1354
 
BbvCI CC'TCA_GC

Cuts at:      0      57    1659 
   Size:         57    1602
 
BclI T'GATC_A

Cuts at:      0    1110    1659 
   Size:       1110     549
 
Bme1580I G_kGCm'C

Cuts at:      0    1069    1174    1659 
   Size:       1069     105     485

  Fragments arranged by size: 

                1069     485     105
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394    1659 
   Size:        394    1265
 
BmtI G_CTAG'C

Cuts at:      0    1514    1659 
   Size:       1514     145
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     383    1659 
   Size:        383    1276
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     472     624    1288    1309    1659 
   Size:        277     195     152     664      21     350

  Fragments arranged by size: 

                 664     350     277     195     152      21
 
Bpu10I CC'TnA_GC

Cuts at:      0      57    1659 
   Size:         57    1602
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     478    1659 
   Size:        256     222    1181

  Fragments arranged by size: 

                1181     256     222
 
BsaAI yAC'GTr

Cuts at:      0     609     924    1659 
   Size:        609     315     735

  Fragments arranged by size: 

                 735     609     315
 
BsaWI w'CCGG_w

Cuts at:      0     959    1214    1659 
   Size:        959     255     445

  Fragments arranged by size: 

                 959     445     255
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121    1186    1216    1476    1506    1659 
   Size:         91      30    1065      30     260      30     153

  Fragments arranged by size: 

                1065     260     153      91      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     139     163     535    1139    1659 
   Size:        139      24     372     604     520

  Fragments arranged by size: 

                 604     520     372     139      24
 
BseYI C'CCAG_C

Cuts at:      0    1373    1538    1659 
   Size:       1373     165     121

  Fragments arranged by size: 

                1373     165     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     551    1659 
   Size:        232     319    1108

  Fragments arranged by size: 

                1108     319     232
 
BsiEI CG_ry'CG

Cuts at:      0      14      68    1659 
   Size:         14      54    1591

  Fragments arranged by size: 

                1591      54      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     503     707     743     842    1082    1659 
   Size:        494       9     204      36      99     240     577

  Fragments arranged by size: 

                 577     494     240     204      99      36       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     494     503     707     743     842     940    1006    1069 
   Size:        494       9     204      36      99      98      66      63

Cuts at:   1069    1082    1108    1174    1389    1659 
   Size:         13      26      66     215     270

  Fragments arranged by size: 

                 494     270     215     204      99      98      66      66
                  63      36      26      13       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     440     716    1591    1659 
   Size:        440     276     875      68

  Fragments arranged by size: 

                 875     440     276      68
 
BsrFI r'CCGG_y

Cuts at:      0      67      93     344     959    1659 
   Size:         67      26     251     615     700

  Fragments arranged by size: 

                 700     615     251      67      26
 
BssHII G'CGCG_C

Cuts at:      0     171    1659 
   Size:        171    1488
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     551    1659 
   Size:        551    1108
 
BstEII G'GTnAC_C

Cuts at:      0    1202    1659 
   Size:       1202     457
 
BstYI r'GATC_y

Cuts at:      0     260    1659 
   Size:        260    1399
 
BtgI C'CryG_G

Cuts at:      0      77     264    1659 
   Size:         77     187    1395

  Fragments arranged by size: 

                1395     187      77
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     211    1659 
   Size:        211    1448
 
BtsI GCAGTG_nn'

Cuts at:      0     142     395     696    1137    1408    1659 
   Size:        142     253     301     441     271     251

  Fragments arranged by size: 

                 441     301     271     253     251     142
 
EaeI y'GGCC_r

Cuts at:      0      11      65    1659 
   Size:         11      54    1594

  Fragments arranged by size: 

                1594      54      11
 
EagI C'GGCC_G

Cuts at:      0      11      65    1659 
   Size:         11      54    1594

  Fragments arranged by size: 

                1594      54      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     196    1169    1659 
   Size:         58     138     973     490

  Fragments arranged by size: 

                 973     490     138      58
 
EcoICRI GAG'CTC

Cuts at:      0     492     501    1659 
   Size:        492       9    1158

  Fragments arranged by size: 

                1158     492       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     472     624    1000    1288    1309    1500    1659 
   Size:        277     195     152     376     288      21     191     159

  Fragments arranged by size: 

                 376     288     277     195     191     159     152      21
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1257    1659 
   Size:       1257     402
 
EcoO109I rG'GnC_Cy

Cuts at:      0     102     318     826    1659 
   Size:        102     216     508     833

  Fragments arranged by size: 

                 833     508     216     102
 
EcoRV GAT'ATC

Cuts at:      0     279    1659 
   Size:        279    1380
 
FspI TGC'GCA

Cuts at:      0     540    1659 
   Size:        540    1119
 
HaeII r_GCGC'y

Cuts at:      0     761    1659 
   Size:        761     898
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     372     404    1095    1127    1186    1218 
   Size:         91      32     249      32     691      32      59      32

Cuts at:   1218    1659 
   Size:        441

  Fragments arranged by size: 

                 691     441     249      91      59      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98    1366    1659 
   Size:         98    1268     293

  Fragments arranged by size: 

                1268     293      98
 
MslI CAynn'nnrTG

Cuts at:      0    1217    1659 
   Size:       1217     442
 
MspA1I CmG'CkG

Cuts at:      0      79     246     266     551    1151    1266    1287    1659 
   Size:         79     167      20     285     600     115      21     372

  Fragments arranged by size: 

                 600     372     285     167     115      79      21      20
            
 
NaeI GCC'GGC

Cuts at:      0      69    1659 
   Size:         69    1590
 
NgoMIV G'CCGG_C

Cuts at:      0      67    1659 
   Size:         67    1592
 
NheI G'CTAG_C

Cuts at:      0    1510    1659 
   Size:       1510     149
 
NspI r_CATG'y

Cuts at:      0    1308    1528    1659 
   Size:       1308     220     131

  Fragments arranged by size: 

                1308     220     131
 
PfoI T'CCnGG_A

Cuts at:      0     293     332    1611    1659 
   Size:        293      39    1279      48

  Fragments arranged by size: 

                1279     293      48      39
 
PmlI CAC'GTG

Cuts at:      0     924    1659 
   Size:        924     735
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1476    1508    1659 
   Size:       1476      32     151

  Fragments arranged by size: 

                1476     151      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     102     318     826    1659 
   Size:        102     216     508     833

  Fragments arranged by size: 

                 833     508     216     102
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1541    1573    1659 
   Size:       1541      32      86

  Fragments arranged by size: 

                1541      86      32
 
PstI C_TGCA'G

Cuts at:      0     137     245     437     713    1509    1602    1659 
   Size:        137     108     192     276     796      93      57

  Fragments arranged by size: 

                 796     276     192     137     108      93      57
 
PvuII CAG'CTG

Cuts at:      0     246    1266    1287    1659 
   Size:        246    1020      21     372

  Fragments arranged by size: 

                1020     372     246      21
 
SacI G_AGCT'C

Cuts at:      0     494     503    1659 
   Size:        494       9    1156

  Fragments arranged by size: 

                1156     494       9
 
SacII CC_GC'GG

Cuts at:      0      80     267    1659 
   Size:         80     187    1392

  Fragments arranged by size: 

                1392     187      80
 
SfcI C'TryA_G

Cuts at:      0     133     241     433     709    1224    1505    1598    1659 
   Size:        133     108     192     276     515     281      93      61

  Fragments arranged by size: 

                 515     281     276     192     133     108      93      61
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0      93    1659 
   Size:         93    1566
 
SmaI CCC'GGG

Cuts at:      0    1451    1659 
   Size:       1451     208
 
SmlI C'TyrA_G

Cuts at:      0     271     493    1567    1659 
   Size:        271     222    1074      92

  Fragments arranged by size: 

                1074     271     222      92
 
SphI G_CATG'C

Cuts at:      0    1308    1659 
   Size:       1308     351
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     610    1512    1659 
   Size:        610     902     147

  Fragments arranged by size: 

                 902     610     147
 
TatI w'GTAC_w

Cuts at:      0    1643    1659 
   Size:       1643      16
 
Tth111I GACn'n_nGTC

Cuts at:      0     386    1659 
   Size:        386    1273
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     688    1659 
   Size:        688     971
 
XmaI C'CCGG_G

Cuts at:      0    1449    1659 
   Size:       1449     210
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AgeI     AloI    AlwNI     AscI
     AvaI     BanI    BanII     BbsI    BbvCI     BclI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI
   BssHII   BstAPI   BstEII    BstYI     BtgI    BtgZI     BtsI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI
    HaeII    Hin4I     MmeI     MslI   MspA1I     NaeI   NgoMIV     NheI
     NspI     PfoI     PmlI     PpiI    PpuMI     PsrI     PstI    PvuII
     SacI    SacII     SfcI    SgrAI     SmaI     SmlI     SphI    TaqII
     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AhdI     AleI     AlfI
     ApaI    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BsaI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI    BssSI    BstBI    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EarI    EcoRI     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NarI     NcoI     NdeI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SnaBI     SpeI     SrfI     SspI
     StuI     StyI     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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