Restriction map for pF1KSDA1882
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AocI        AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SexAI       SfiI        
SfuI        SnaBI       SpeI        SspBI       SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI         1402  GTCTAC               GT^[AC][GT]AC
AcyI         3039  GGCGCC               G[GA]^CG[CT]C
AgeI         1590  ACCGGT               A^CCGGT
AhdI         1477  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1157  AGCGCT               AGC^GCT
AspEI        1477  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3235  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1329  GGATCC               G^GATCC
BbeI         3039  GGCGCC               GGCGC^C
BbvCI          28  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2475  GCAGGTCGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI        3039  GGCGCC               G[GA]^CG[CT]C
BspLU11I      680  ACATGT               A^CATGT
BsrDI        2126  CATTGC               GCAATG(2/0)
Eam1105I     1477  GACTTCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1157  AGCGCT               AGC^GCT
EcoNI        1409  CCTCCCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I        3039  GGCGCC               G[GA]^CG[CT]C
HincII       1695  GTCAAC               GT[CT]^[GA]AC
HindII       1695  GTCAAC               GT[CT]^[GA]AC
KasI         3039  GGCGCC               G^GCGCC
NaeI         2794  GCCGGC               GCC^GGC
NarI         3039  GGCGCC               GG^CGCC
NcoI         1550  CCATGG               C^CATGG
NgoMIV       2794  GCCGGC               G^CCGGC
NheI         1648  GCTAGC               G^CTAGC
PflFI        3235  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1590  ACCGGT               A^CCGGT
ScaI          922  AGTACT               AGT^ACT
SfoI         3039  GGCGCC               GGC^GCC
SmaI         2595  CCCGGG               CCC^GGG
SmlI         2298  CTTGAG               C^T[CT][GA]AG
SphI          611  GCATGC               GCATG^C
SspI         2218  AATATT               AAT^ATT
StuI         2922  AGGCCT               AGG^CCT
Tth111I      3235  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2595  CCCGGG               C^CCGGG
XmaI         2595  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I       3214  GTGCAC               G^TGCAC
             3415  GTGCAC              
ApaI         3166  GGGCCC               GGGCC^C
             3311  GGGCCC              
ApaLI        3214  GTGCAC               G^TGCAC
             3415  GTGCAC              
BalI          274  TGGCCA               TGG^CCA
             2354  TGGCCA              
BglI         3104  GCCAGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3427  GCCCTGGAGGC         
BglII         311  AGATCT               A^GATCT
             2113  AGATCT              
BsmBI          75  CGTCTC               CGTCTC(1/5)
             3240  CGTCTC              
BsmI         1014  GAATGC               GAATGC(1/-1)
             1107  GAATGC              
BsrBI        1750  GAGCGG               CCGCTC(-3/-3)
             2905  GAGCGG              
BssSI        3361  CACGAG               CACGAG(-5/-1)
               49  CTCGTG              
BstEII       1981  GGTTACC              G^GT[ACGT]ACC
             2250  GGTCACC             
BstPI        1981  GGTTACC              G^GT[ACGT]ACC
             2250  GGTCACC             
EcoO65I      1981  GGTTACC              G^GT[ACGT]ACC
             2250  GGTCACC             
HindIII      1606  AAGCTT               A^AGCTT
             1674  AAGCTT              
MluNI         274  TGGCCA               TGG^CCA
             2354  TGGCCA              
MscI          274  TGGCCA               TGG^CCA
             2354  TGGCCA              
MseI         1121  TTAA                 T^TAA
             2207  TTAA                
PpuMI         629  GGGACCT              [GA]G^G[AT]CC[CT]
             1946  GGGTCCC             
PshAI        1445  GACACCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1935  GACGCTCGTC          
SacI          800  GAGCTC               GAGCT^C
             1177  GAGCTC              
SapI         1747  GAAGAGC              GCTCTTC(1/4)
             2902  GAAGAGC             
SbfI          639  CCTGCAGG             CCTGCA^GG
             2412  CCTGCAGG            
SgrAI        1589  CACCGGTG             C[GA]^CCGG[CT]G
             2793  CGCCGGCG            
Sse8387I      639  CCTGCAGG             CCTGCA^GG
             2412  CCTGCAGG            
Tru9I        1121  TTAA                 T^TAA
             2207  TTAA                
XcmI         1235  CCATGCAGTGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2172  CCAGCTTTACAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbrPI       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PmaCI       PmlI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com