Restriction map for pF1KSDA1882
 (Linear) (Six-base) MAPSORT of: KIAA1882.seq  Check: 5036  from: 1  to: 3444



KIAA1882.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2723    3038    3444 
   Size:       2723     315     406

  Fragments arranged by size: 

                2723     406     315
 
AccI GT'mk_AC

Cuts at:      0    1403    3444 
   Size:       1403    2041
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     854     980    1087    2627    2992    3444 
   Size:         78     776     126     107    1540     365     452

  Fragments arranged by size: 

                1540     776     452     365     126     107      78
 
AfeI AGC'GCT

Cuts at:      0    1159    3444 
   Size:       1159    2285
 
AflIII A'CryG_T

Cuts at:      0     680    1056    1691    3419    3444 
   Size:        680     376     635    1728      25

  Fragments arranged by size: 

                1728     680     635     376      25
 
AgeI A'CCGG_T

Cuts at:      0    1590    3444 
   Size:       1590    1854
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1482    3444 
   Size:       1482    1962
 
AleI CACnn'nnGTG

Cuts at:      0     391    1739    3422    3444 
   Size:        391    1348    1683      22

  Fragments arranged by size: 

                1683    1348     391      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2122    3400    3440    3444 
   Size:       2122    1278      40       4

  Fragments arranged by size: 

                2122    1278      40       4
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1516    1548    3444 
   Size:       1516      32    1896

  Fragments arranged by size: 

                1896    1516      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45     757    1364    2015    3444 
   Size:         45     712     607     651    1429

  Fragments arranged by size: 

                1429     712     651     607      45
 
ApaI G_GGCC'C

Cuts at:      0    3170    3315    3444 
   Size:       3170     145     129

  Fragments arranged by size: 

                3170     145     129
 
ApaLI G'TGCA_C

Cuts at:      0    3214    3415    3444 
   Size:       3214     201      29

  Fragments arranged by size: 

                3214     201      29
 
ApoI r'AATT_y

Cuts at:      0    1096    1151    2574    3444 
   Size:       1096      55    1423     870

  Fragments arranged by size: 

                1423    1096     870      55
 
AvaI C'yCGr_G

Cuts at:      0     187    2489    2518    2595    2672    2918    3307    3444 
   Size:        187    2302      29      77      77     246     389     137

  Fragments arranged by size: 

                2302     389     246     187     137      77      77      29
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2147    2180    3444 
   Size:       2147      33    1264

  Fragments arranged by size: 

                2147    1264      33
 
BamHI G'GATC_C

Cuts at:      0    1329    3444 
   Size:       1329    2115
 
BanI G'GyrC_C

Cuts at:      0     173     599     886    1593    3039    3444 
   Size:        173     426     287     707    1446     405

  Fragments arranged by size: 

                1446     707     426     405     287     173
 
BanII G_rGCy'C

Cuts at:      0     804    1181    1559    3154    3170    3256    3315    3444 
   Size:        804     377     378    1595      16      86      59     129

  Fragments arranged by size: 

                1595     804     378     377     129      86      59      16
            
 
BbeI G_GCGC'C

Cuts at:      0    3043    3444 
   Size:       3043     401
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     342     968    1989    2560    2721    3444 
   Size:        342     626    1021     571     161     723

  Fragments arranged by size: 

                1021     723     626     571     342     161
 
BbvCI CC'TCA_GC

Cuts at:      0      29    3444 
   Size:         29    3415
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2464    2498    3444 
   Size:       2464      34     946

  Fragments arranged by size: 

                2464     946      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3110    3433    3444 
   Size:       3110     323      11

  Fragments arranged by size: 

                3110     323      11
 
BglII A'GATC_T

Cuts at:      0     311    2113    3444 
   Size:        311    1802    1331

  Fragments arranged by size: 

                1802    1331     311
 
Bme1580I G_kGCm'C

Cuts at:      0     864     891    1598    3170    3218    3315    3419    3429 
   Size:        864      27     707    1572      48      97     104      10

Cuts at:   3429    3444 
   Size:         15

  Fragments arranged by size: 

                1572     864     707     104      97      48      27      15
                  10
 
BmgBI CAC'GTC

Cuts at:      0     393     937    3444 
   Size:        393     544    2507

  Fragments arranged by size: 

                2507     544     393
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1924    2322    3404    3444 
   Size:       1924     398    1082      40

  Fragments arranged by size: 

                1924    1082     398      40
 
BmtI G_CTAG'C

Cuts at:      0    1652    3444 
   Size:       1652    1792
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     818    2271    3444 
   Size:        818    1453    1173

  Fragments arranged by size: 

                1453    1173     818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    2266    2609    2917    2947    2976    3043    3148 
   Size:        496    1770     343     308      30      29      67     105

Cuts at:   3148    3240    3444 
   Size:         92     204

  Fragments arranged by size: 

                1770     496     343     308     204     105      92      67
                  30      29
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     952    3444 
   Size:         29     923    2492

  Fragments arranged by size: 

                2492     923      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2319    3444 
   Size:       2319    1125
 
BsaAI yAC'GTr

Cuts at:      0     300     624    1057    2550    3420    3444 
   Size:        300     324     433    1493     870      24

  Fragments arranged by size: 

                1493     870     433     324     300      24
 
BsaHI Gr'CG_yC

Cuts at:      0    3040    3444 
   Size:       3040     404
 
BsaWI w'CCGG_w

Cuts at:      0    1590    3146    3353    3444 
   Size:       1590    1556     207      91

  Fragments arranged by size: 

                1590    1556     207      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1255    1285    1867    1897    3226    3256    3444 
   Size:       1255      30     582      30    1329      30     188

  Fragments arranged by size: 

                1329    1255     582     188      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97    1282    3307    3444 
   Size:         97    1185    2025     137

  Fragments arranged by size: 

                2025    1185     137      97
 
BseYI C'CCAG_C

Cuts at:      0     146    1364    1680    2688    2943    3444 
   Size:        146    1218     316    1008     255     501

  Fragments arranged by size: 

                1218    1008     501     316     255     146
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322    2102    2773    3214    3301    3444 
   Size:        322    1780     671     441      87     143

  Fragments arranged by size: 

                1780     671     441     322     143      87
 
BsiHKAI G_wGCw'C

Cuts at:      0     804    1181    3029    3218    3419    3444 
   Size:        804     377    1848     189     201      25

  Fragments arranged by size: 

                1848     804     377     201     189      25
 
BsmI GAATG_Cn'

Cuts at:      0    1020    1113    3444 
   Size:       1020      93    2331

  Fragments arranged by size: 

                2331    1020      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      81    3246    3444 
   Size:         81    3165     198

  Fragments arranged by size: 

                3165     198      81
 
Bsp1286I G_dGCh'C

Cuts at:      0     804     864     891    1181    1559    1598    3029    3154 
   Size:        804      60      27     290     378      39    1431     125

Cuts at:   3154    3170    3218    3256    3315    3419    3429    3444 
   Size:         16      48      38      59     104      10      15

  Fragments arranged by size: 

                1431     804     378     290     125     104      60      59
                  48      39      38      27      16      15      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2039    2466    2723    3038    3236    3444 
   Size:       2039     427     257     315     198     208

  Fragments arranged by size: 

                2039     427     315     257     208     198
 
BsrBI CCG'CTC

Cuts at:      0    1752    2907    3444 
   Size:       1752    1155     537

  Fragments arranged by size: 

                1752    1155     537
 
BsrDI GCAATG_nn'

Cuts at:      0    2125    3444 
   Size:       2125    1319
 
BsrFI r'CCGG_y

Cuts at:      0     851    1590    2794    3444 
   Size:        851     739    1204     650

  Fragments arranged by size: 

                1204     851     739     650
 
BssSI C'ACGA_G

Cuts at:      0      49    3361    3444 
   Size:         49    3312      83

  Fragments arranged by size: 

                3312      83      49
 
BstEII G'GTnAC_C

Cuts at:      0    1981    2250    3444 
   Size:       1981     269    1194

  Fragments arranged by size: 

                1981    1194     269
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     489    1872    2895    3395    3444 
   Size:        489    1383    1023     500      49

  Fragments arranged by size: 

                1383    1023     500     489      49
 
BstYI r'GATC_y

Cuts at:      0      98     311     521    1329    2113    2418    2930    3444 
   Size:         98     213     210     808     784     305     512     514

  Fragments arranged by size: 

                 808     784     514     512     305     213     210      98
            
 
BtgI C'CryG_G

Cuts at:      0    1550    1740    1991    3444 
   Size:       1550     190     251    1453

  Fragments arranged by size: 

                1550    1453     251     190
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1186    1242    1545    3444 
   Size:       1186      56     303    1899

  Fragments arranged by size: 

                1899    1186     303      56
 
BtsI GCAGTG_nn'

Cuts at:      0      28    1246    2713    2985    3055    3444 
   Size:         28    1218    1467     272      70     389

  Fragments arranged by size: 

                1467    1218     389     272      70      28
 
EaeI y'GGCC_r

Cuts at:      0     263     274     853    1229    1657    2354    3291    3444 
   Size:        263      11     579     376     428     697     937     153

  Fragments arranged by size: 

                 937     697     579     428     376     263     153      11
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     430     787    1139    1742    1910    2897    3325    3444 
   Size:        430     357     352     603     168     987     428     119

  Fragments arranged by size: 

                 987     603     430     428     357     352     168     119
            
 
EcoICRI GAG'CTC

Cuts at:      0     802    1179    3444 
   Size:        802     377    2265

  Fragments arranged by size: 

                2265     802     377
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     496     854     980    1087    2266    2609    2627 
   Size:         78     418     358     126     107    1179     343      18

Cuts at:   2627    2917    2947    2976    2992    3043    3148    3240    3444 
   Size:        290      30      29      16      51     105      92     204

  Fragments arranged by size: 

                1179     418     358     343     290     204     126     107
                 105      92      78      51      30      29      18      16
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1413    3444 
   Size:       1413    2031
 
EcoO109I rG'GnC_Cy

Cuts at:      0     630    1277    1947    3166    3167    3311    3312    3444 
   Size:        630     647     670    1219       1     144       1     132

  Fragments arranged by size: 

                1219     670     647     630     144     132       1       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2853    3444 
   Size:       2853     591
 
HaeII r_GCGC'y

Cuts at:      0    1161    1900    3043    3444 
   Size:       1161     739    1143     401

  Fragments arranged by size: 

                1161    1143     739     401
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     244     276    1468    1500    1867    1899    2078    2110 
   Size:        244      32    1192      32     367      32     179      32

Cuts at:   2110    2137    2169    3226    3258    3444 
   Size:         27      32    1057      32     186

  Fragments arranged by size: 

                1192    1057     367     244     186     179      32      32
                  32      32      32      32      27
 
HincII GTy'rAC

Cuts at:      0    1697    3444 
   Size:       1697    1747
 
HindIII A'AGCT_T

Cuts at:      0    1606    1674    3444 
   Size:       1606      68    1770

  Fragments arranged by size: 

                1770    1606      68
 
KasI G'GCGC_C

Cuts at:      0    3039    3444 
   Size:       3039     405
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1466    3444 
   Size:       1466    1978
 
MscI TGG'CCA

Cuts at:      0     276    2356    3444 
   Size:        276    2080    1088

  Fragments arranged by size: 

                2080    1088     276
 
MslI CAynn'nnrTG

Cuts at:      0     391     685    1739    2377    3422    3444 
   Size:        391     294    1054     638    1045      22

  Fragments arranged by size: 

                1054    1045     638     391     294      22
 
MspA1I CmG'CkG

Cuts at:      0    1806    1868    2895    3036    3444 
   Size:       1806      62    1027     141     408

  Fragments arranged by size: 

                1806    1027     408     141      62
 
NaeI GCC'GGC

Cuts at:      0    2796    3444 
   Size:       2796     648
 
NarI GG'CG_CC

Cuts at:      0    3040    3444 
   Size:       3040     404
 
NcoI C'CATG_G

Cuts at:      0    1550    3444 
   Size:       1550    1894
 
NgoMIV G'CCGG_C

Cuts at:      0    2794    3444 
   Size:       2794     650
 
NheI G'CTAG_C

Cuts at:      0    1648    3444 
   Size:       1648    1796
 
NspI r_CATG'y

Cuts at:      0      21     615     684    1627    2644    3444 
   Size:         21     594      69     943    1017     800

  Fragments arranged by size: 

                1017     943     800     594      69      21
 
PasI CC'CwG_GG

Cuts at:      0    2631    2860    3444 
   Size:       2631     229     584

  Fragments arranged by size: 

                2631     584     229
 
PciI A'CATG_T

Cuts at:      0     680    3444 
   Size:        680    2764
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      66     272     429    1398    2811    3444 
   Size:         66     206     157     969    1413     633

  Fragments arranged by size: 

                1413     969     633     206     157      66
 
PfoI T'CCnGG_A

Cuts at:      0    1295    2720    2849    3020    3194    3444 
   Size:       1295    1425     129     171     174     250

  Fragments arranged by size: 

                1425    1295     250     174     171     129
 
PmlI CAC'GTG

Cuts at:      0     300     624    2550    3420    3444 
   Size:        300     324    1926     870      24

  Fragments arranged by size: 

                1926     870     324     300      24
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480    3444 
   Size:        448      32    2964

  Fragments arranged by size: 

                2964     448      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     630    1947    3444 
   Size:        630    1317    1497

  Fragments arranged by size: 

                1497    1317     630
 
PshAI GACnn'nnGTC

Cuts at:      0    1449    1939    3444 
   Size:       1449     490    1505

  Fragments arranged by size: 

                1505    1449     490
 
PspOMI G'GGCC_C

Cuts at:      0    3166    3311    3444 
   Size:       3166     145     133

  Fragments arranged by size: 

                3166     145     133
 
PstI C_TGCA'G

Cuts at:      0     362     644     659    2093    2417    3035    3050    3444 
   Size:        362     282      15    1434     324     618      15     394

  Fragments arranged by size: 

                1434     618     394     362     324     282      15      15
            
 
PvuII CAG'CTG

Cuts at:      0    1806    2895    3036    3444 
   Size:       1806    1089     141     408

  Fragments arranged by size: 

                1806    1089     408     141
 
SacI G_AGCT'C

Cuts at:      0     804    1181    3444 
   Size:        804     377    2263

  Fragments arranged by size: 

                2263     804     377
 
SanDI GG'GwC_CC

Cuts at:      0    1947    3444 
   Size:       1947    1497
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1742    2897    3444 
   Size:       1742    1155     547

  Fragments arranged by size: 

                1742    1155     547
 
SbfI CC_TGCA'GG

Cuts at:      0     644    2417    3444 
   Size:        644    1773    1027

  Fragments arranged by size: 

                1773    1027     644
 
ScaI AGT'ACT

Cuts at:      0     924    3444 
   Size:        924    2520
 
SfcI C'TryA_G

Cuts at:      0     168     358     640     655    1161    1314    1462    2089 
   Size:        168     190     282      15     506     153     148     627

Cuts at:   2089    2413    2541    3031    3046    3444 
   Size:        324     128     490      15     398

  Fragments arranged by size: 

                 627     506     490     398     324     282     190     168
                 153     148     128      15      15
 
SfoI GGC'GCC

Cuts at:      0    3041    3444 
   Size:       3041     403
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1590    2794    3444 
   Size:       1590    1204     650

  Fragments arranged by size: 

                1590    1204     650
 
SmaI CCC'GGG

Cuts at:      0    2597    3444 
   Size:       2597     847
 
SmlI C'TyrA_G

Cuts at:      0    2298    3444 
   Size:       2298    1146
 
SphI G_CATG'C

Cuts at:      0     615    3444 
   Size:        615    2829
 
SrfI GCCC'GGGC

Cuts at:      0    2597    3444 
   Size:       2597     847
 
SspI AAT'ATT

Cuts at:      0    2220    3444 
   Size:       2220    1224
 
StuI AGG'CCT

Cuts at:      0    2924    3444 
   Size:       2924     520
 
StyI C'CwwG_G

Cuts at:      0     266     603    1550    3444 
   Size:        266     337     947    1894

  Fragments arranged by size: 

                1894     947     337     266
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1259    3444 
   Size:       1259    2185
 
TatI w'GTAC_w

Cuts at:      0     922    3444 
   Size:        922    2522
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     730     762    1255    1287    1593    1625    2426    2458 
   Size:        730      32     493      32     306      32     801      32

Cuts at:   2458    3444 
   Size:        986

  Fragments arranged by size: 

                 986     801     730     493     306      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3238    3444 
   Size:       3238     206
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1242    2179    3444 
   Size:       1242     937    1265

  Fragments arranged by size: 

                1265    1242     937
 
XmaI C'CCGG_G

Cuts at:      0    2595    3444 
   Size:       2595     849
 
XmnI GAAnn'nnTTC

Cuts at:      0      91    1100    2069    3444 
   Size:         91    1009     969    1375

  Fragments arranged by size: 

                1375    1009     969      91
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI    SgrAI
     SmaI     SmlI     SphI     SrfI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AscI     AseI    AsiSI    AvrII
    BciVI     BclI    BfrBI     BlpI     BsaI    BsaBI    BsiEI    BsiWI
    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
     NdeI     NotI     NruI     NsiI     PacI     PmeI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SexAI     SfiI     SgfI
    SnaBI     SpeI     SwaI     XbaI     XhoI     ZraI
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