Restriction map for pF1KSDA1712
 (Linear) (Six-base) MAPSORT of: KIAA1712.seq  Check: 6434  from: 1  to: 1170



KIAA1712.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     361     662    1170 
   Size:         89     272     301     508

  Fragments arranged by size: 

                 508     301     272      89
 
AflII C'TTAA_G

Cuts at:      0     742    1170 
   Size:        742     428
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64    1170 
   Size:         32      32    1106

  Fragments arranged by size: 

                1106      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     463    1170 
   Size:        463     707
 
ApoI r'AATT_y

Cuts at:      0     892    1170 
   Size:        892     278
 
BanII G_rGCy'C

Cuts at:      0     194    1170 
   Size:        194     976
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     367     926    1170 
   Size:        367     559     244

  Fragments arranged by size: 

                 559     367     244
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     558    1170 
   Size:        558     612
 
BclI T'GATC_A

Cuts at:      0     246    1170 
   Size:        246     924
 
BlpI GC'TnA_GC

Cuts at:      0     668    1170 
   Size:        668     502
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      95    1170 
   Size:         95    1075
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      32      62    1170 
   Size:         32      30    1108

  Fragments arranged by size: 

                1108      32      30
 
BseYI C'CCAG_C

Cuts at:      0     105    1170 
   Size:        105    1065
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    1170 
   Size:        689     481
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     194    1170 
   Size:         50     144     976

  Fragments arranged by size: 

                 976     144      50
 
BsmI GAATG_Cn'

Cuts at:      0     733    1170 
   Size:        733     437
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     194    1170 
   Size:         50     144     976

  Fragments arranged by size: 

                 976     144      50
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     476    1170 
   Size:        476     694
 
BsrDI GCAATG_nn'

Cuts at:      0     447    1170 
   Size:        447     723
 
Bsu36I CC'TnA_GG

Cuts at:      0     498    1170 
   Size:        498     672
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     857    1170 
   Size:         65     792     313

  Fragments arranged by size: 

                 792     313      65
 
EcoICRI GAG'CTC

Cuts at:      0     192    1170 
   Size:        192     978
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     361     662    1170 
   Size:         89     272     301     508

  Fragments arranged by size: 

                 508     301     272      89
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      40    1170 
   Size:         40    1130
 
EcoRV GAT'ATC

Cuts at:      0     477    1170 
   Size:        477     693
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     757    1170 
   Size:        757     413
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468    1170 
   Size:        436      32     702

  Fragments arranged by size: 

                 702     436      32
 
HincII GTy'rAC

Cuts at:      0     552    1170 
   Size:        552     618
 
HindIII A'AGCT_T

Cuts at:      0      24     235    1170 
   Size:         24     211     935

  Fragments arranged by size: 

                 935     211      24
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1104    1170 
   Size:       1104      66
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64    1170 
   Size:         32      32    1106

  Fragments arranged by size: 

                1106      32      32
 
PsiI TTA'TAA

Cuts at:      0     172     260    1170 
   Size:        172      88     910

  Fragments arranged by size: 

                 910     172      88
 
PstI C_TGCA'G

Cuts at:      0     459    1140    1170 
   Size:        459     681      30

  Fragments arranged by size: 

                 681     459      30
 
SacI G_AGCT'C

Cuts at:      0     194    1170 
   Size:        194     976
 
SfcI C'TryA_G

Cuts at:      0     455    1011    1136    1170 
   Size:        455     556     125      34

  Fragments arranged by size: 

                 556     455     125      34
 
SmlI C'TyrA_G

Cuts at:      0     742    1170 
   Size:        742     428
 
TatI w'GTAC_w

Cuts at:      0    1015    1170 
   Size:       1015     155
 

 Enzymes that do cut:

     AcuI    AflII     AloI    AlwNI     ApoI    BanII     BbsI    BciVI
     BclI     BlpI     BsaI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI   Bsu36I     EarI  EcoICRI  Eco57MI    EcoNI
    EcoRV     FalI    Hin4I   HincII  HindIII    PflMI     PpiI     PsiI
     PstI     SacI     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI EcoO109I    EcoRI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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