Restriction map for pF1KSDA1709
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BciVI       BfrI        
BlnI        BsaHI       BsgI        BsiEI       BsiWI       
BsmBI       BspDI       BspHI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       FauI        FseI        
FspI        Hin1I       HpaI        KasI        KpnI        
KspI        MluI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspI        
SwaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        1370  CGCG                 CG^CG
AflIII        125  ACATGT               A^C[GA][CT]GT
AocI          825  CCTGAGG              CC^T[ACGT]AGG
Asp700I      2304  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1821  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          401  TGGCCA               TGG^CCA
BanII        2779  GAGCCC               G[GA]GC[CT]^C
BbvCI         702  GCTGAGG              CCTCAGC(-5/-2)
BclI         1064  TGATCA               T^GATCA
BglII          67  AGATCT               A^GATCT
BlpI           55  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       55  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1597  TACGTG               [CT]AC^GT[GA]
BsaBI        1358  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          325  GAGACC               GGTCTC(1/5)
Bsp1407I      479  TGTACA               T^GTACA
BspLU11I      125  ACATGT               A^CATGT
BsrBI          58  GAGCGG               CCGCTC(-3/-3)
BsrDI        1884  CATTGC               GCAATG(2/0)
BsrFI        1259  GCCGGT               [GA]^CCGG[CT]
BsrGI         479  TGTACA               T^GTACA
BssSI        2232  CTCGTG               CACGAG(-5/-1)
BstAPI       1935  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1370  CGCG                 CG^CG
Bsu36I        825  CCTGAGG              CC^T[ACGT]AGG
CelII          55  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1259  GCCGGT               [GA]^CCGG[CT]
DraI          979  TTTAAA               TTT^AAA
Eco81I        825  CCTGAGG              CC^T[ACGT]AGG
EcoT22I         1  ATGCAT               ATGCA^T
FbaI         1064  TGATCA               T^GATCA
HaeII        1303  AGCGCC               [GA]GCGC^[CT]
HgaI         2250  GACGC                GACGC(5/10)
HincII       1295  GTCAAC               GT[CT]^[GA]AC
HindII       1295  GTCAAC               GT[CT]^[GA]AC
HindIII      1508  AAGCTT               A^AGCTT
MamI         1358  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          475  CAATTG               C^AATTG
MluNI         401  TGGCCA               TGG^CCA
MscI          401  TGGCCA               TGG^CCA
MunI          475  CAATTG               C^AATTG
MvnI         1370  CGCG                 CG^CG
NsiI            1  ATGCAT               ATGCA^T
PflFI        1821  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScaI          832  AGTACT               AGT^ACT
SpeI         1149  ACTAGT               A^CTAGT
SspBI         479  TGTACA               T^GTACA
Tth111I      1821  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          771  TCTAGA               T^CTAGA
XmnI         2304  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccIII         29  TCCGGA               T^CCGGA
              669  TCCGGA              
AlwNI        1121  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1936  CAGCTGCTG           
AspHI        1928  GTGCTC               G[AT]GC[AT]^C
             2227  GAGCAC              
BbsI         2247  GAAGAC               GAAGAC(2/6)
             2790  GAAGAC              
BcgI         1647  CGATGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              344  GCAATACCTTCG        
BglI         1199  GCCTACTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1354  GCCAGATTGGC         
BpuAI        2247  GAAGAC               GAAGAC(2/6)
             2790  GAAGAC              
BseAI          29  TCCGGA               T^CCGGA
              669  TCCGGA              
BsiHKAI      1928  GTGCTC               G[AT]GC[AT]^C
             2227  GAGCAC              
BspEI          29  TCCGGA               T^CCGGA
              669  TCCGGA              
CfoI         1304  GCGC                 GCG^C
             1369  GCGC                
HhaI         1304  GCGC                 GCG^C
             1369  GCGC                
HinP1I       1304  GCGC                 G^CGC
             1369  GCGC                
MaeII         635  ACGT                 A^CGT
             1598  ACGT                
MroI           29  TCCGGA               T^CCGGA
              669  TCCGGA              
NcoI          644  CCATGG               C^CATGG
             1816  CCATGG              
NspI          125  ACATGT               [GA]CATG^[CT]
              356  GCATGT              
PaeR7I        854  CTCGAG               C^TCGAG
             1784  CTCGAG              
StuI         1559  AGGCCT               AGG^CCT
             2101  AGGCCT              
XhoI          854  CTCGAG               C^TCGAG
             1784  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BsaWI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       
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