Restriction map for pF1KSDA1709
 (Linear) (Six-base) MAPSORT of: KIAA1709.seq  Check: 3509  from: 1  to: 3075



KIAA1709.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1662    3075 
   Size:       1662    1413
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143    1526    1586    1784    2266    2485    2884    3075 
   Size:        143    1383      60     198     482     219     399     191

  Fragments arranged by size: 

                1383     482     399     219     198     191     143      60
            
 
AflIII A'CryG_T

Cuts at:      0     125    3075 
   Size:        125    2950
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     379    3075 
   Size:        379    2696
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1862    1894    3075 
   Size:       1862      32    1181

  Fragments arranged by size: 

                1862    1181      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1126    1941    3075 
   Size:       1126     815    1134

  Fragments arranged by size: 

                1134    1126     815
 
ApoI r'AATT_y

Cuts at:      0     796     970    1048    2006    2827    3075 
   Size:        796     174      78     958     821     248

  Fragments arranged by size: 

                 958     821     796     248     174      78
 
AvaI C'yCGr_G

Cuts at:      0     854    1784    2661    3075 
   Size:        854     930     877     414

  Fragments arranged by size: 

                 930     877     854     414
 
BanII G_rGCy'C

Cuts at:      0    2783    3075 
   Size:       2783     292
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2254    2797    3075 
   Size:       2254     543     278

  Fragments arranged by size: 

                2254     543     278
 
BbvCI CC'TCA_GC

Cuts at:      0     703    3075 
   Size:        703    2372
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     333     367    1636    1670    3075 
   Size:        333      34    1269      34    1405

  Fragments arranged by size: 

                1405    1269     333      34      34
 
BclI T'GATC_A

Cuts at:      0    1064    3075 
   Size:       1064    2011
 
BfrBI ATG'CAT

Cuts at:      0       3    3075 
   Size:          3    3072
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1205    1360    3075 
   Size:       1205     155    1715

  Fragments arranged by size: 

                1715    1205     155
 
BglII A'GATC_T

Cuts at:      0      67    3075 
   Size:         67    3008
 
BlpI GC'TnA_GC

Cuts at:      0      56    3075 
   Size:         56    3019
 
Bme1580I G_kGCm'C

Cuts at:      0    1255    1691    3075 
   Size:       1255     436    1384

  Fragments arranged by size: 

                1384    1255     436
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     828    1558    2676    3075 
   Size:        828     730    1118     399

  Fragments arranged by size: 

                1118     828     730     399
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718    1378    1618    1996    2494    3075 
   Size:        718     660     240     378     498     581

  Fragments arranged by size: 

                 718     660     581     498     378     240
 
Bpu10I CC'TnA_GC

Cuts at:      0     703    2422    3075 
   Size:        703    1719     653

  Fragments arranged by size: 

                1719     703     653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    1258    2011    2143    3075 
   Size:        454     804     753     132     932

  Fragments arranged by size: 

                 932     804     753     454     132
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     319    3075 
   Size:        319    2756
 
BsaAI yAC'GTr

Cuts at:      0    1599    3075 
   Size:       1599    1476
 
BsaBI GATnn'nnATC

Cuts at:      0    1362    3075 
   Size:       1362    1713
 
BsaWI w'CCGG_w

Cuts at:      0      29     203     669    2221    2687    3075 
   Size:         29     174     466    1552     466     388

  Fragments arranged by size: 

                1552     466     466     388     174      29
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2343    2373    2435    2465    3075 
   Size:       2343      30      62      30     610

  Fragments arranged by size: 

                2343     610      62      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     618     843    2074    2146    2446    2758    3075 
   Size:        618     225    1231      72     300     312     317

  Fragments arranged by size: 

                1231     618     317     312     300     225      72
 
BseYI C'CCAG_C

Cuts at:      0     186     734     895    1134    2411    2559    2935    3075 
   Size:        186     548     161     239    1277     148     376     140

  Fragments arranged by size: 

                1277     548     376     239     186     161     148     140
            
 
BsiHKAI G_wGCw'C

Cuts at:      0    1932    2231    3075 
   Size:       1932     299     844

  Fragments arranged by size: 

                1932     844     299
 
BsmI GAATG_Cn'

Cuts at:      0     221     501    1707    3075 
   Size:        221     280    1206    1368

  Fragments arranged by size: 

                1368    1206     280     221
 
Bsp1286I G_dGCh'C

Cuts at:      0    1255    1691    1932    2231    2783    3075 
   Size:       1255     436     241     299     552     292

  Fragments arranged by size: 

                1255     552     436     299     292     241
 
BspEI T'CCGG_A

Cuts at:      0      29     669    3075 
   Size:         29     640    2406

  Fragments arranged by size: 

                2406     640      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1662    1952    2078    3075 
   Size:       1662     290     126     997

  Fragments arranged by size: 

                1662     997     290     126
 
BsrBI CCG'CTC

Cuts at:      0      60    3075 
   Size:         60    3015
 
BsrDI GCAATG_nn'

Cuts at:      0    1883    3075 
   Size:       1883    1192
 
BsrFI r'CCGG_y

Cuts at:      0    1259    3075 
   Size:       1259    1816
 
BsrGI T'GTAC_A

Cuts at:      0     479    3075 
   Size:        479    2596
 
BssSI C'ACGA_G

Cuts at:      0    2232    3075 
   Size:       2232     843
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1941    3075 
   Size:       1941    1134
 
BstYI r'GATC_y

Cuts at:      0      67     335    1842    3075 
   Size:         67     268    1507    1233

  Fragments arranged by size: 

                1507    1233     268      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     826    3075 
   Size:        826    2249
 
BtgI C'CryG_G

Cuts at:      0     644    1816    2895    3075 
   Size:        644    1172    1079     180

  Fragments arranged by size: 

                1172    1079     644     180
 
BtsI GCAGTG_nn'

Cuts at:      0    1067    1325    1375    1433    3075 
   Size:       1067     258      50      58    1642

  Fragments arranged by size: 

                1642    1067     258      58      50
 
DraI TTT'AAA

Cuts at:      0     981    3075 
   Size:        981    2094
 
EaeI y'GGCC_r

Cuts at:      0     401    1257    2528    3075 
   Size:        401     856    1271     547

  Fragments arranged by size: 

                1271     856     547     401
 
EarI CTCTTCn'nnn_

Cuts at:      0     289    1189    1672    2039    2452    2600    2860    2903 
   Size:        289     900     483     367     413     148     260      43

Cuts at:   2903    3075 
   Size:        172

  Fragments arranged by size: 

                 900     483     413     367     289     260     172     148
                  43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     718    1378    1526    1586    1618    1784    1996 
   Size:        143     575     660     148      60      32     166     212

Cuts at:   1996    2266    2485    2494    2884    3075 
   Size:        270     219       9     390     191

  Fragments arranged by size: 

                 660     575     390     270     219     212     191     166
                 148     143      60      32       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     651    1806    1986    2067    2565    3075 
   Size:        651    1155     180      81     498     510

  Fragments arranged by size: 

                1155     651     510     498     180      81
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     738    3075 
   Size:        738    2337
 
HaeII r_GCGC'y

Cuts at:      0    1307    3075 
   Size:       1307    1768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     669     701    3075 
   Size:        669      32    2374

  Fragments arranged by size: 

                2374     669      32
 
HincII GTy'rAC

Cuts at:      0    1297    3075 
   Size:       1297    1778
 
HindIII A'AGCT_T

Cuts at:      0    1508    3075 
   Size:       1508    1567
 
MfeI C'AATT_G

Cuts at:      0     475    3075 
   Size:        475    2600
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1602    2021    2337    3075 
   Size:       1602     419     316     738

  Fragments arranged by size: 

                1602     738     419     316
 
MscI TGG'CCA

Cuts at:      0     403    3075 
   Size:        403    2672
 
MslI CAynn'nnrTG

Cuts at:      0     361     530    2518    3075 
   Size:        361     169    1988     557

  Fragments arranged by size: 

                1988     557     361     169
 
MspA1I CmG'CkG

Cuts at:      0     225     850    1938    2559    2637    3075 
   Size:        225     625    1088     621      78     438

  Fragments arranged by size: 

                1088     625     621     438     225      78
 
NcoI C'CATG_G

Cuts at:      0     644    1816    3075 
   Size:        644    1172    1259

  Fragments arranged by size: 

                1259    1172     644
 
NsiI A_TGCA'T

Cuts at:      0       5    3075 
   Size:          5    3070
 
NspI r_CATG'y

Cuts at:      0     129     360    3075 
   Size:        129     231    2715

  Fragments arranged by size: 

                2715     231     129
 
PasI CC'CwG_GG

Cuts at:      0     879    1417    3075 
   Size:        879     538    1658

  Fragments arranged by size: 

                1658     879     538
 
PciI A'CATG_T

Cuts at:      0     125    3075 
   Size:        125    2950
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     741    1852    2673    3075 
   Size:        741    1111     821     402

  Fragments arranged by size: 

                1111     821     741     402
 
PfoI T'CCnGG_A

Cuts at:      0    1394    3075 
   Size:       1394    1681
 
PpuMI rG'GwC_Cy

Cuts at:      0    1806    1986    2067    2565    3075 
   Size:       1806     180      81     498     510

  Fragments arranged by size: 

                1806     510     498     180      81
 
PsiI TTA'TAA

Cuts at:      0     176    3075 
   Size:        176    2899
 
PspXI vC'TCGA_Gb

Cuts at:      0     854    3075 
   Size:        854    2221
 
PstI C_TGCA'G

Cuts at:      0     230     371     876    1122    1937    1946    2618    3075 
   Size:        230     141     505     246     815       9     672     457

  Fragments arranged by size: 

                 815     672     505     457     246     230     141       9
            
 
PvuII CAG'CTG

Cuts at:      0     225     850    1938    2559    2637    3075 
   Size:        225     625    1088     621      78     438

  Fragments arranged by size: 

                1088     625     621     438     225      78
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1189    2600    2860    3075 
   Size:       1189    1411     260     215

  Fragments arranged by size: 

                1411    1189     260     215
 
ScaI AGT'ACT

Cuts at:      0     834    3075 
   Size:        834    2241
 
SfcI C'TryA_G

Cuts at:      0     226     367     410     872    1118    1933    1942    2614 
   Size:        226     141      43     462     246     815       9     672

Cuts at:   2614    3075 
   Size:        461

  Fragments arranged by size: 

                 815     672     462     461     246     226     141      43
                   9
 
SmlI C'TyrA_G

Cuts at:      0     469     854    1273    1784    1990    2158    3075 
   Size:        469     385     419     511     206     168     917

  Fragments arranged by size: 

                 917     511     469     419     385     206     168
 
SpeI A'CTAG_T

Cuts at:      0    1149    3075 
   Size:       1149    1926
 
StuI AGG'CCT

Cuts at:      0    1561    2103    3075 
   Size:       1561     542     972

  Fragments arranged by size: 

                1561     972     542
 
StyI C'CwwG_G

Cuts at:      0     644    1187    1816    3075 
   Size:        644     543     629    1259

  Fragments arranged by size: 

                1259     644     629     543
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     733     748    1711    1910    2116    3075 
   Size:        733      15     963     199     206     959

  Fragments arranged by size: 

                 963     959     733     206     199      15
 
TatI w'GTAC_w

Cuts at:      0     479     832    1520    1949    3075 
   Size:        479     353     688     429    1126

  Fragments arranged by size: 

                1126     688     479     429     353
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147    2435    2467    3075 
   Size:        115      32    2288      32     608

  Fragments arranged by size: 

                2288     608     115      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1824    3075 
   Size:       1824    1251
 
XbaI T'CTAG_A

Cuts at:      0     771    3075 
   Size:        771    2304
 
XhoI C'TCGA_G

Cuts at:      0     854    1784    3075 
   Size:        854     930    1291

  Fragments arranged by size: 

                1291     930     854
 
XmnI GAAnn'nnTTC

Cuts at:      0    2308    3075 
   Size:       2308     767
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI    AlwNI     ApoI     AvaI
    BanII     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstYI   Bsu36I     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NsiI     NspI     PasI     PciI
    PflMI     PfoI    PpuMI     PsiI    PspXI     PstI    PvuII     SapI
     ScaI     SfcI     SmlI     SpeI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BciVI    BmgBI     BmrI     BmtI    BsaHI
     BsgI    BsiEI    BsiWI    BsmBI    BspHI   BssHII    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PmeI     PmlI     PpiI    PshAI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     SwaI     XcmI     XmaI     ZraI
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