Restriction map for pF1KSDA1706
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbsI        BcgI        BciVI       BclI        BfrI        
BlnI        BpuAI       BsaBI       BsaI        BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    EarI        Eco47III    EcoNI       EcoO65I     
EcoRI       EcoT22I     FbaI        FseI        FspI        
KpnI        Ksp632I     KspI        MamI        MboII       
MfeI        MunI        NcoI        NdeI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tsp509I     
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII       1554  TCCGGA               T^CCGGA
AfaI          198  GTAC                 GT^AC
AgeI         1271  ACCGGT               A^CCGGT
AocI         1380  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1497  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         569  GGATCC               G^GATCC
BbeI          259  GGCGCC               GGCGC^C
BbrPI         404  CACGTG               CAC^GTG
BglII         290  AGATCT               A^GATCT
BlpI          314  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      314  GCTCAGC              GC^T[ACGT]AGC
BsaAI         404  CACGTG               [CT]AC^GT[GA]
BseAI        1554  TCCGGA               T^CCGGA
BsgI          933  CTGCAC               GTGCAG(16/14)
BsiEI         733  CGGCCG               CG[GA][CT]^CG
BsmBI        1094  GAGACG               CGTCTC(1/5)
BspEI        1554  TCCGGA               T^CCGGA
BspHI         440  TCATGA               T^CATGA
BsrDI        1364  GCAATG               GCAATG(2/0)
BssSI        1699  CACGAG               CACGAG(-5/-1)
BstAPI        897  GCAAGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         263  CCACCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1380  CCTGAGG              CC^T[ACGT]AGG
CelII         314  GCTCAGC              GC^T[ACGT]AGC
DraIII        577  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          733  CGGCCG               C^GGCCG
EclXI         733  CGGCCG               C^GGCCG
Eco52I        733  CGGCCG               C^GGCCG
Eco81I       1380  CCTGAGG              CC^T[ACGT]AGG
EcoRV        1375  GATATC               GAT^ATC
HaeII         259  GGCGCC               [GA]GCGC^[CT]
KasI          259  GGCGCC               G^GCGCC
MaeII         405  ACGT                 A^CGT
MluI          169  ACGCGT               A^CGCGT
MroI         1554  TCCGGA               T^CCGGA
NaeI         1078  GCCGGC               GCC^GGC
NarI          259  GGCGCC               GG^CGCC
NgoMIV       1078  GCCGGC               G^CCGGC
PflMI        1269  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1271  ACCGGT               A^CCGGT
PmaCI         404  CACGTG               CAC^GTG
PmlI          404  CACGTG               CAC^GTG
PstI          676  CTGCAG               CTGCA^G
PvuII         386  CAGCTG               CAG^CTG
RcaI          440  TCATGA               T^CATGA
RsaI          198  GTAC                 GT^AC
SacI         1113  GAGCTC               GAGCT^C
SexAI         578  ACCAGGT              A^CC[AT]GGT
SfoI          259  GGCGCC               GGC^GCC
SmlI         1448  CTCAAG               C^T[CT][GA]AG
Van91I       1269  CCACCGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1035  CCAGAAGGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1497  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcyI          259  GGCGCC               G[GA]^CG[CT]C
             1642  GACGCC              
AflIII        169  ACGCGT               A^C[GA][CT]GT
              405  ACGTGT              
AspHI           6  GAGCAC               G[AT]GC[AT]^C
             1113  GAGCTC              
BanI          259  GGCGCC               G^G[CT][GA]CC
              575  GGCACC              
BbvCI         474  CCTCAGC              CCTCAGC(-5/-2)
              780  GCTGAGG             
BglI          424  GCCACCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              702  GCCAGGGGGGC         
BsaHI         259  GGCGCC               G[GA]^CG[CT]C
             1642  GACGCC              
BsaWI        1271  ACCGGT               [AT]^CCGG[AT]
             1554  TCCGGA              
BseRI         139  GAGGAG               GAGGAG(10/8)
              142  GAGGAG              
BsiHKAI         6  GAGCAC               G[AT]GC[AT]^C
             1113  GAGCTC              
BsmAI        1094  GAGAC                GTCTC(1/5)
             1461  GTCTC               
BsrBI          24  CCGCTC               CCGCTC(-3/-3)
              112  GAGCGG              
BsrFI        1078  GCCGGC               [GA]^CCGG[CT]
             1271  ACCGGT              
BstYI         290  AGATCT               [GA]^GATC[CT]
              569  GGATCC              
Cfr10I       1078  GCCGGC               [GA]^CCGG[CT]
             1271  ACCGGT              
Hin1I         259  GGCGCC               G[GA]^CG[CT]C
             1642  GACGCC              
HincII        409  GTTAAC               GT[CT]^[GA]AC
             1201  GTTAAC              
HindII        409  GTTAAC               GT[CT]^[GA]AC
             1201  GTTAAC              
HindIII       883  AAGCTT               A^AGCTT
             1488  AAGCTT              
HpaI          409  GTTAAC               GTT^AAC
             1201  GTTAAC              
MflI          290  AGATCT               [GA]^GATC[CT]
              569  GGATCC              
MspA1I        386  CAGCTG               C[AC]G^C[GT]G
             1659  CAGCGG              
NheI          354  GCTAGC               G^CTAGC
             1602  GCTAGC              
SfcI          676  CTGCAG               C^T[GA][CT]AG
             1297  CTACAG              
SmaI          835  CCCGGG               CCC^GGG
              986  CCCGGG              
TaqI         1024  TCGA                 T^CGA
             1456  TCGA                
TthHB8I      1024  TCGA                 T^CGA
             1456  TCGA                
XhoII         290  AGATCT               [GA]^GATC[CT]
              569  GGATCC              
XmaCI         835  CCCGGG               C^CCGGG
              986  CCCGGG              
XmaI          835  CCCGGG               C^CCGGG
              986  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        ApaI        
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       Sau3AI      Sau96I      ScrFI       SfaNI       
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      TspRI       
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