Restriction map for pF1KSDA1706
 (Linear) (Six-base) MAPSORT of: KIAA1706.seq  Check: 674  from: 1  to: 1707



KIAA1706.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72    1707 
   Size:         72    1635
 
AflIII A'CryG_T

Cuts at:      0     169     405    1707 
   Size:        169     236    1302

  Fragments arranged by size: 

                1302     236     169
 
AgeI A'CCGG_T

Cuts at:      0    1271    1707 
   Size:       1271     436
 
AleI CACnn'nnGTG

Cuts at:      0     388    1707 
   Size:        388    1319
 
ApaI G_GGCC'C

Cuts at:      0     509     713     986    1150    1707 
   Size:        509     204     273     164     557

  Fragments arranged by size: 

                 557     509     273     204     164
 
AvaI C'yCGr_G

Cuts at:      0     654     835     986    1162    1707 
   Size:        654     181     151     176     545

  Fragments arranged by size: 

                 654     545     181     176     151
 
BamHI G'GATC_C

Cuts at:      0     569    1707 
   Size:        569    1138
 
BanI G'GyrC_C

Cuts at:      0     259     575    1707 
   Size:        259     316    1132

  Fragments arranged by size: 

                1132     316     259
 
BanII G_rGCy'C

Cuts at:      0     509     661     713     986    1117    1150    1707 
   Size:        509     152      52     273     131      33     557

  Fragments arranged by size: 

                 557     509     273     152     131      52      33
 
BbeI G_GCGC'C

Cuts at:      0     263    1707 
   Size:        263    1444
 
BbvCI CC'TCA_GC

Cuts at:      0     475     781    1707 
   Size:        475     306     926

  Fragments arranged by size: 

                 926     475     306
 
BglI GCCn_nnn'nGGC

Cuts at:      0     430     708    1707 
   Size:        430     278     999

  Fragments arranged by size: 

                 999     430     278
 
BglII A'GATC_T

Cuts at:      0     290    1707 
   Size:        290    1417
 
BlpI GC'TnA_GC

Cuts at:      0     315    1707 
   Size:        315    1392
 
Bme1580I G_kGCm'C

Cuts at:      0     395     509     713     986    1133    1150    1707 
   Size:        395     114     204     273     147      17     557

  Fragments arranged by size: 

                 557     395     273     204     147     114      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     326    1524    1591    1707 
   Size:        326    1198      67     116

  Fragments arranged by size: 

                1198     326     116      67
 
BmtI G_CTAG'C

Cuts at:      0     358    1606    1707 
   Size:        358    1248     101

  Fragments arranged by size: 

                1248     358     101
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1038    1552    1707 
   Size:       1038     514     155

  Fragments arranged by size: 

                1038     514     155
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    1018    1650    1707 
   Size:        292     726     632      57

  Fragments arranged by size: 

                 726     632     292      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     475     670     781    1707 
   Size:        475     195     111     926

  Fragments arranged by size: 

                 926     475     195     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1433    1707 
   Size:       1433     274
 
BsaAI yAC'GTr

Cuts at:      0     406    1707 
   Size:        406    1301
 
BsaHI Gr'CG_yC

Cuts at:      0     260    1643    1707 
   Size:        260    1383      64

  Fragments arranged by size: 

                1383     260      64
 
BsaWI w'CCGG_w

Cuts at:      0    1271    1554    1707 
   Size:       1271     283     153

  Fragments arranged by size: 

                1271     283     153
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     154     157    1707 
   Size:        154       3    1550

  Fragments arranged by size: 

                1550     154       3
 
BseYI C'CCAG_C

Cuts at:      0     434    1045    1150    1246    1707 
   Size:        434     611     105      96     461

  Fragments arranged by size: 

                 611     461     434     105      96
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     918    1707 
   Size:        918     789
 
BsiEI CG_ry'CG

Cuts at:      0     736    1707 
   Size:        736     971
 
BsiHKAI G_wGCw'C

Cuts at:      0      10    1117    1707 
   Size:         10    1107     590

  Fragments arranged by size: 

                1107     590      10
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1088    1707 
   Size:       1088     619
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     395     509     661     713     986    1117    1133 
   Size:         10     385     114     152      52     273     131      16

Cuts at:   1133    1150    1707 
   Size:         17     557

  Fragments arranged by size: 

                 557     385     273     152     131     114      52      17
                  16      10
 
BspEI T'CCGG_A

Cuts at:      0    1554    1707 
   Size:       1554     153
 
BspHI T'CATG_A

Cuts at:      0     440    1707 
   Size:        440    1267
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     267     359     704    1707 
   Size:        267      92     345    1003

  Fragments arranged by size: 

                1003     345     267      92
 
BsrBI CCG'CTC

Cuts at:      0      26     114    1707 
   Size:         26      88    1593

  Fragments arranged by size: 

                1593      88      26
 
BsrDI GCAATG_nn'

Cuts at:      0    1371    1707 
   Size:       1371     336
 
BsrFI r'CCGG_y

Cuts at:      0    1078    1271    1707 
   Size:       1078     193     436

  Fragments arranged by size: 

                1078     436     193
 
BssSI C'ACGA_G

Cuts at:      0    1699    1707 
   Size:       1699       8
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     903    1707 
   Size:        903     804
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270    1707 
   Size:        270    1437
 
BstYI r'GATC_y

Cuts at:      0     290     569    1707 
   Size:        290     279    1138

  Fragments arranged by size: 

                1138     290     279
 
Bsu36I CC'TnA_GG

Cuts at:      0    1381    1707 
   Size:       1381     326
 
BtgI C'CryG_G

Cuts at:      0     134     176     740     815    1707 
   Size:        134      42     564      75     892

  Fragments arranged by size: 

                 892     564     134      75      42
 
BtsI GCAGTG_nn'

Cuts at:      0     128    1590    1707 
   Size:        128    1462     117

  Fragments arranged by size: 

                1462     128     117
 
DraIII CAC_nnn'GTG

Cuts at:      0     582    1707 
   Size:        582    1125
 
EaeI y'GGCC_r

Cuts at:      0     380     733     788    1266    1347    1707 
   Size:        380     353      55     478      81     360

  Fragments arranged by size: 

                 478     380     360     353      81      55
 
EagI C'GGCC_G

Cuts at:      0     733    1707 
   Size:        733     974
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     481    1707 
   Size:        481    1226
 
EcoICRI GAG'CTC

Cuts at:      0    1115    1707 
   Size:       1115     592
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     292    1018    1650    1707 
   Size:         72     220     726     632      57

  Fragments arranged by size: 

                 726     632     220      72      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     234     348     506     525     602     687     709 
   Size:         39     195     114     158      19      77      85      22

Cuts at:    709     982     983    1147    1707 
   Size:        273       1     164     560

  Fragments arranged by size: 

                 560     273     195     164     158     114      85      77
                  39      22      19       1
 
EcoRV GAT'ATC

Cuts at:      0    1377    1707 
   Size:       1377     330
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     922    1707 
   Size:        922     785
 
HaeII r_GCGC'y

Cuts at:      0     263    1707 
   Size:        263    1444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     194     226    1227    1259    1549    1581    1707 
   Size:        194      32    1001      32     290      32     126

  Fragments arranged by size: 

                1001     290     194     126      32      32      32
 
HincII GTy'rAC

Cuts at:      0     411    1203    1707 
   Size:        411     792     504

  Fragments arranged by size: 

                 792     504     411
 
HindIII A'AGCT_T

Cuts at:      0     883    1488    1707 
   Size:        883     605     219

  Fragments arranged by size: 

                 883     605     219
 
HpaI GTT'AAC

Cuts at:      0     411    1203    1707 
   Size:        411     792     504

  Fragments arranged by size: 

                 792     504     411
 
KasI G'GCGC_C

Cuts at:      0     259    1707 
   Size:        259    1448
 
MluI A'CGCG_T

Cuts at:      0     169    1707 
   Size:        169    1538
 
MscI TGG'CCA

Cuts at:      0     382     790    1268    1349    1707 
   Size:        382     408     478      81     358

  Fragments arranged by size: 

                 478     408     382     358      81
 
MslI CAynn'nnrTG

Cuts at:      0     100     388     445    1262    1707 
   Size:        100     288      57     817     445

  Fragments arranged by size: 

                 817     445     288     100      57
 
MspA1I CmG'CkG

Cuts at:      0     388    1661    1707 
   Size:        388    1273      46

  Fragments arranged by size: 

                1273     388      46
 
NaeI GCC'GGC

Cuts at:      0    1080    1707 
   Size:       1080     627
 
NarI GG'CG_CC

Cuts at:      0     260    1707 
   Size:        260    1447
 
NgoMIV G'CCGG_C

Cuts at:      0    1078    1707 
   Size:       1078     629
 
NheI G'CTAG_C

Cuts at:      0     354    1602    1707 
   Size:        354    1248     105

  Fragments arranged by size: 

                1248     354     105
 
PasI CC'CwG_GG

Cuts at:      0      12      35    1707 
   Size:         12      23    1672

  Fragments arranged by size: 

                1672      23      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1275    1707 
   Size:       1275     432
 
PfoI T'CCnGG_A

Cuts at:      0     560     866    1707 
   Size:        560     306     841

  Fragments arranged by size: 

                 841     560     306
 
PmlI CAC'GTG

Cuts at:      0     406    1707 
   Size:        406    1301
 
PpuMI rG'GwC_Cy

Cuts at:      0      39     234     348     525     687    1707 
   Size:         39     195     114     177     162    1020

  Fragments arranged by size: 

                1020     195     177     162     114      39
 
PspOMI G'GGCC_C

Cuts at:      0     505     709     982    1146    1707 
   Size:        505     204     273     164     561

  Fragments arranged by size: 

                 561     505     273     204     164
 
PstI C_TGCA'G

Cuts at:      0     680    1707 
   Size:        680    1027
 
PvuII CAG'CTG

Cuts at:      0     388    1707 
   Size:        388    1319
 
SacI G_AGCT'C

Cuts at:      0    1117    1707 
   Size:       1117     590
 
SanDI GG'GwC_CC

Cuts at:      0      39    1707 
   Size:         39    1668
 
SexAI A'CCwGG_T

Cuts at:      0     578    1707 
   Size:        578    1129
 
SfcI C'TryA_G

Cuts at:      0     676    1297    1707 
   Size:        676     621     410

  Fragments arranged by size: 

                 676     621     410
 
SfoI GGC'GCC

Cuts at:      0     261    1707 
   Size:        261    1446
 
SmaI CCC'GGG

Cuts at:      0     837     988    1707 
   Size:        837     151     719

  Fragments arranged by size: 

                 837     719     151
 
SmlI C'TyrA_G

Cuts at:      0    1448    1707 
   Size:       1448     259
 
StuI AGG'CCT

Cuts at:      0     455     748     774     919    1535    1707 
   Size:        455     293      26     145     616     172

  Fragments arranged by size: 

                 616     455     293     172     145      26
 
StyI C'CwwG_G

Cuts at:      0     920    1350    1546    1670    1707 
   Size:        920     430     196     124      37

  Fragments arranged by size: 

                 920     430     196     124      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     628     730    1707 
   Size:        628     102     977

  Fragments arranged by size: 

                 977     628     102
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1042    1707 
   Size:       1042     665
 
XmaI C'CCGG_G

Cuts at:      0     835     986    1707 
   Size:        835     151     721

  Fragments arranged by size: 

                 835     721     151
 
XmnI GAAnn'nnTTC

Cuts at:      0    1501    1707 
   Size:       1501     206
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AleI     ApaI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MluI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NheI     PasI    PflMI     PfoI     PmlI    PpuMI
   PspOMI     PstI    PvuII     SacI    SanDI    SexAI     SfcI     SfoI
     SmaI     SmlI     StuI     StyI    TaqII     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI    BmgBI
     BsaI    BsaBI    BsaXI    BsiWI     BsmI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EarI
    EcoNI    EcoRI     FseI     FspI    FspAI     KpnI     MfeI     MmeI
     NcoI     NdeI     NotI     NruI     NsiI     NspI     PacI     PciI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com