Restriction map for pF1KSDA1704
 (Linear) (Six-base) MAPSORT of: KIAA1704.seq  Check: 5873  from: 1  to: 1020



KIAA1704.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     887    1020 
   Size:        212     675     133

  Fragments arranged by size: 

                 675     212     133
 
ApaI G_GGCC'C

Cuts at:      0     504    1020 
   Size:        504     516
 
AvaI C'yCGr_G

Cuts at:      0      78    1020 
   Size:         78     942
 
BanII G_rGCy'C

Cuts at:      0      85     504    1020 
   Size:         85     419     516

  Fragments arranged by size: 

                 516     419      85
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     158     875    1020 
   Size:        158     717     145

  Fragments arranged by size: 

                 717     158     145
 
BglII A'GATC_T

Cuts at:      0     915    1020 
   Size:        915     105
 
BlpI GC'TnA_GC

Cuts at:      0     782    1020 
   Size:        782     238
 
Bme1580I G_kGCm'C

Cuts at:      0     504    1020 
   Size:        504     516
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     662     671    1020 
   Size:        572      90       9     349

  Fragments arranged by size: 

                 572     349      90       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     701    1020 
   Size:        701     319
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1020 
   Size:        904     116
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       2    1020 
   Size:          2    1018
 
BsaBI GATnn'nnATC

Cuts at:      0     819     914    1020 
   Size:        819      95     106

  Fragments arranged by size: 

                 819     106      95
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     660     690    1020 
   Size:        660      30     330

  Fragments arranged by size: 

                 660     330      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     305    1020 
   Size:        305     715
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     504    1020 
   Size:         85     419     516

  Fragments arranged by size: 

                 516     419      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     777    1020 
   Size:        777     243
 
BsrBI CCG'CTC

Cuts at:      0      72    1020 
   Size:         72     948
 
BsrDI GCAATG_nn'

Cuts at:      0     340    1020 
   Size:        340     680
 
BsrFI r'CCGG_y

Cuts at:      0      21    1020 
   Size:         21     999
 
BstYI r'GATC_y

Cuts at:      0     915    1020 
   Size:        915     105
 
BtgI C'CryG_G

Cuts at:      0      48    1020 
   Size:         48     972
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     161    1020 
   Size:        161     859
 
BtsI GCAGTG_nn'

Cuts at:      0     426    1020 
   Size:        426     594
 
ClaI AT'CG_AT

Cuts at:      0     657    1020 
   Size:        657     363
 
DraI TTT'AAA

Cuts at:      0     297    1020 
   Size:        297     723
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     627     759    1020 
   Size:         62     565     132     261

  Fragments arranged by size: 

                 565     261     132      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     572     662     671     887    1020 
   Size:        212     360      90       9     216     133

  Fragments arranged by size: 

                 360     216     212     133      90       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      89     455    1020 
   Size:         89     366     565

  Fragments arranged by size: 

                 565     366      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     334     500    1020 
   Size:         75     259     166     520

  Fragments arranged by size: 

                 520     259     166      75
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31     219     251     529     561     631     660     663 
   Size:         31     188      32     278      32      70      29       3

Cuts at:    663     692     775     807    1020 
   Size:         29      83      32     213

  Fragments arranged by size: 

                 278     213     188      83      70      32      32      32
                  31      29      29       3
 
HincII GTy'rAC

Cuts at:      0     468    1020 
   Size:        468     552
 
HindIII A'AGCT_T

Cuts at:      0     880    1020 
   Size:        880     140
 
HpaI GTT'AAC

Cuts at:      0     468    1020 
   Size:        468     552
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     235    1020 
   Size:        235     785
 
MspA1I CmG'CkG

Cuts at:      0      50      58     682    1020 
   Size:         50       8     624     338

  Fragments arranged by size: 

                 624     338      50       8
 
PfoI T'CCnGG_A

Cuts at:      0     288     396    1020 
   Size:        288     108     624

  Fragments arranged by size: 

                 624     288     108
 
PpuMI rG'GwC_Cy

Cuts at:      0      75     334    1020 
   Size:         75     259     686

  Fragments arranged by size: 

                 686     259      75
 
PshAI GACnn'nnGTC

Cuts at:      0     206     605    1020 
   Size:        206     399     415

  Fragments arranged by size: 

                 415     399     206
 
PsiI TTA'TAA

Cuts at:      0     116    1020 
   Size:        116     904
 
PspOMI G'GGCC_C

Cuts at:      0     500    1020 
   Size:        500     520
 
PvuII CAG'CTG

Cuts at:      0     682    1020 
   Size:        682     338
 
RsrII CG'GwC_CG

Cuts at:      0      19    1020 
   Size:         19    1001
 
SacII CC_GC'GG

Cuts at:      0      51    1020 
   Size:         51     969
 
SanDI GG'GwC_CC

Cuts at:      0      75    1020 
   Size:         75     945
 
SapI GCTCTTCn'nnn_

Cuts at:      0      62     627    1020 
   Size:         62     565     393

  Fragments arranged by size: 

                 565     393      62
 
SexAI A'CCwGG_T

Cuts at:      0     348    1020 
   Size:        348     672
 
SmlI C'TyrA_G

Cuts at:      0     919    1020 
   Size:        919     101
 
StyI C'CwwG_G

Cuts at:      0     319     616    1020 
   Size:        319     297     404

  Fragments arranged by size: 

                 404     319     297
 
XbaI T'CTAG_A

Cuts at:      0     970    1020 
   Size:        970      50
 

 Enzymes that do cut:

     AcuI     ApaI     AvaI    BanII     BbsI    BglII     BlpI Bme1580I
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BstYI     BtgI    BtgZI     BtsI
     ClaI     DraI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII
  HindIII     HpaI     MmeI   MspA1I     PfoI    PpuMI    PshAI     PsiI
   PspOMI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SmlI
     StyI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmrI
     BmtI     BplI    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PspXI     PsrI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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