Restriction map for pF1KSDA1674
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmFI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        MamI        MroI        NarI        
NciI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1478  GTCGAC               GT^[AC][GT]AC
AgeI          561  ACCGGT               A^CCGGT
AlwNI        1048  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1499  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          591  CCCGAG               C^[CT]CG[GA]G
BalI         1365  TGGCCA               TGG^CCA
BbrPI         814  CACGTG               CAC^GTG
BbsI          930  GAAGAC               GAAGAC(2/6)
BfaI           71  CTAG                 C^TAG
BglII        1197  AGATCT               A^GATCT
BlpI         1137  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1137  GCTCAGC              GC^T[ACGT]AGC
BpuAI         930  GAAGAC               GAAGAC(2/6)
BsaAI         814  CACGTG               [CT]AC^GT[GA]
BsaI          617  GAGACC               GGTCTC(1/5)
BsgI          469  GTGCAG               GTGCAG(16/14)
BsmBI        1068  GAGACG               CGTCTC(1/5)
BsmI          228  GAATGC               GAATGC(1/-1)
BsoBI         591  CCCGAG               C^[CT]CG[GA]G
BsrBI        1016  GAGCGG               CCGCTC(-3/-3)
CelII        1137  GCTCAGC              GC^T[ACGT]AGC
EaeI         1365  TGGCCA               [CT]^GGCC[GA]
EcoNI         938  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         448  GAATTC               G^AATTC
HgaI          625  GCGTC                GACGC(5/10)
HincII       1478  GTCGAC               GT[CT]^[GA]AC
HindII       1478  GTCGAC               GT[CT]^[GA]AC
KspI         1156  CCGCGG               CCGC^GG
MaeI           71  CTAG                 C^TAG
MfeI          211  CAATTG               C^AATTG
MluI          623  ACGCGT               A^CGCGT
MluNI        1365  TGGCCA               TGG^CCA
MscI         1365  TGGCCA               TGG^CCA
MslI          106  CACAAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1156  CCGCGG               C[AC]G^C[GT]G
MunI          211  CAATTG               C^AATTG
NaeI         1518  GCCGGC               GCC^GGC
NcoI         1362  CCATGG               C^CATGG
NgoMIV       1518  GCCGGC               G^CCGGC
PinAI         561  ACCGGT               A^CCGGT
PleI          742  GACTC                GAGTC(4/5)
PmaCI         814  CACGTG               CAC^GTG
PmlI          814  CACGTG               CAC^GTG
PpuMI         653  AGGACCC              [GA]G^G[AT]CC[CT]
SacII        1156  CCGCGG               CCGC^GG
SalI         1478  GTCGAC               G^TCGAC
SapI         1132  GAAGAGC              GCTCTTC(1/4)
SbfI         1293  CCTGCAGG             CCTGCA^GG
Sse8387I     1293  CCTGCAGG             CCTGCA^GG
TaqI         1479  TCGA                 T^CGA
TthHB8I      1479  TCGA                 T^CGA
XmnI         1499  GAAACCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         624  CGCG                 CG^CG
             1157  CGCG                
AcsI          448  GAATTC               [GA]^AATT[CT]
             1305  AAATTT              
AflIII        623  ACGCGT               A^C[GA][CT]GT
              915  ACACGT              
AhdI          619  GACCACGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              961  GACCTCTTGTC         
AlwI          987  GATCC                GGATC(4/5)
             1404  GGATC               
Aor51HI       312  AGCGCT               AGC^GCT
              494  AGCGCT              
ApoI          448  GAATTC               [GA]^AATT[CT]
             1305  AAATTT              
AspEI         619  GACCACGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              961  GACCTCTTGTC         
AvaII         288  GGACC                G^G[AT]CC
              654  GGACC               
BbvCI         457  CCTCAGC              CCTCAGC(-5/-2)
              792  GCTGAGG             
BsaWI         293  TCCGGT               [AT]^CCGG[AT]
              561  ACCGGT              
BsmAI         617  GAGAC                GTCTC(1/5)
             1068  GAGAC               
BsrFI         561  ACCGGT               [GA]^CCGG[CT]
             1518  GCCGGC              
BstUI         624  CGCG                 CG^CG
             1157  CGCG                
BstYI        1197  AGATCT               [GA]^GATC[CT]
             1404  GGATCT              
Cfr10I        561  ACCGGT               [GA]^CCGG[CT]
             1518  GCCGGC              
DraII         653  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1370  AGGCCCC             
DsaI         1156  CCGCGG               C^C[GA][CT]GG
             1362  CCATGG              
Eam1105I      619  GACCACGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              961  GACCTCTTGTC         
Eco47III      312  AGCGCT               AGC^GCT
              494  AGCGCT              
EcoO109I      653  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1370  AGGCCCC             
EcoT14I       633  CCAAGG               C^C[AT][AT]GG
             1362  CCATGG              
MflI         1197  AGATCT               [GA]^GATC[CT]
             1404  GGATCT              
MvnI          624  CGCG                 CG^CG
             1157  CGCG                
NlaIII       1167  CATG                 CATG^
             1363  CATG                
PstI         1087  CTGCAG               CTGCA^G
             1294  CTGCAG              
StyI          633  CCAAGG               C^C[AT][AT]GG
             1362  CCATGG              
Tsp45I        584  GTGAC                ^GT[GC]AC
              969  GTCAC               
XhoII        1197  AGATCT               [GA]^GATC[CT]
             1404  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       BanII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIV       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       
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