Restriction map for pF1KSDA1674
 (Linear) (Six-base) MAPSORT of: KIAA1674.seq  Check: 7204  from: 1  to: 1590



KIAA1674.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AccI GT'mk_AC

Cuts at:      0    1479    1590 
   Size:       1479     111
 
AfeI AGC'GCT

Cuts at:      0     314     496    1590 
   Size:        314     182    1094

  Fragments arranged by size: 

                1094     314     182
 
AflIII A'CryG_T

Cuts at:      0     623     915    1590 
   Size:        623     292     675

  Fragments arranged by size: 

                 675     623     292
 
AgeI A'CCGG_T

Cuts at:      0     561    1590 
   Size:        561    1029
 
AhdI GACnn_n'nnGTC

Cuts at:      0     624     966    1590 
   Size:        624     342     624

  Fragments arranged by size: 

                 624     624     342
 
AleI CACnn'nnGTG

Cuts at:      0     110    1590 
   Size:        110    1480
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     245    1165    1590 
   Size:        245     920     425

  Fragments arranged by size: 

                 920     425     245
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     512     544    1590 
   Size:        512      32    1046

  Fragments arranged by size: 

                1046     512      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1053    1590 
   Size:       1053     537
 
ApoI r'AATT_y

Cuts at:      0     448    1305    1590 
   Size:        448     857     285

  Fragments arranged by size: 

                 857     448     285
 
AvaI C'yCGr_G

Cuts at:      0     591    1590 
   Size:        591     999
 
BanII G_rGCy'C

Cuts at:      0     336     598    1037    1110    1139    1590 
   Size:        336     262     439      73      29     451

  Fragments arranged by size: 

                 451     439     336     262      73      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     937    1590 
   Size:        937     653
 
BbvCI CC'TCA_GC

Cuts at:      0     458     793    1590 
   Size:        458     335     797

  Fragments arranged by size: 

                 797     458     335
 
BglII A'GATC_T

Cuts at:      0    1197    1590 
   Size:       1197     393
 
BlpI GC'TnA_GC

Cuts at:      0    1138    1590 
   Size:       1138     452
 
Bme1580I G_kGCm'C

Cuts at:      0    1086    1293    1590 
   Size:       1086     207     297

  Fragments arranged by size: 

                1086     297     207
 
BmgBI CAC'GTC

Cuts at:      0    1332    1590 
   Size:       1332     258
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     859    1590 
   Size:        859     731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     625     635     927    1021    1399    1590 
   Size:         70     555      10     292      94     378     191

  Fragments arranged by size: 

                 555     378     292     191      94      70      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     458     650     793    1590 
   Size:        458     192     143     797

  Fragments arranged by size: 

                 797     458     192     143
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     233     533     856    1590 
   Size:        174      59     300     323     734

  Fragments arranged by size: 

                 734     323     300     174      59
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     611    1590 
   Size:        611     979
 
BsaAI yAC'GTr

Cuts at:      0     816    1590 
   Size:        816     774
 
BsaWI w'CCGG_w

Cuts at:      0     293     561    1590 
   Size:        293     268    1029

  Fragments arranged by size: 

                1029     293     268
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     512     542    1590 
   Size:        512      30    1048

  Fragments arranged by size: 

                1048     512      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     126     196     446     735     781     784     930 
   Size:         67      59      70     250     289      46       3     146

Cuts at:    930    1176    1396    1590 
   Size:        246     220     194

  Fragments arranged by size: 

                 289     250     246     220     194     146      70      67
                  59      46       3
 
BseYI C'CCAG_C

Cuts at:      0     199     396     760    1590 
   Size:        199     197     364     830

  Fragments arranged by size: 

                 830     364     199     197
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490    1590 
   Size:        490    1100
 
BsiHKAI G_wGCw'C

Cuts at:      0     336     815    1037    1139    1590 
   Size:        336     479     222     102     451

  Fragments arranged by size: 

                 479     451     336     222     102
 
BsmI GAATG_Cn'

Cuts at:      0     234    1590 
   Size:        234    1356
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1062    1590 
   Size:       1062     528
 
Bsp1286I G_dGCh'C

Cuts at:      0     336     598     815    1037    1086    1110    1139    1293 
   Size:        336     262     217     222      49      24      29     154

Cuts at:   1293    1590 
   Size:        297

  Fragments arranged by size: 

                 336     297     262     222     217     154      49      29
                  24
 
BsrBI CCG'CTC

Cuts at:      0    1018    1590 
   Size:       1018     572
 
BsrFI r'CCGG_y

Cuts at:      0     561    1518    1590 
   Size:        561     957      72

  Fragments arranged by size: 

                 957     561      72
 
BstYI r'GATC_y

Cuts at:      0    1197    1404    1590 
   Size:       1197     207     186

  Fragments arranged by size: 

                1197     207     186
 
BtgI C'CryG_G

Cuts at:      0    1156    1362    1590 
   Size:       1156     206     228

  Fragments arranged by size: 

                1156     228     206
 
BtsI GCAGTG_nn'

Cuts at:      0     435    1084    1590 
   Size:        435     649     506

  Fragments arranged by size: 

                 649     506     435
 
EaeI y'GGCC_r

Cuts at:      0    1365    1590 
   Size:       1365     225
 
EarI CTCTTCn'nnn_

Cuts at:      0    1001    1117    1127    1307    1590 
   Size:       1001     116      10     180     283

  Fragments arranged by size: 

                1001     283     180     116      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1220    1590 
   Size:       1220     370
 
EcoICRI GAG'CTC

Cuts at:      0     334    1035    1137    1590 
   Size:        334     701     102     453

  Fragments arranged by size: 

                 701     453     334     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     625     635     927    1021    1399    1590 
   Size:         70     555      10     292      94     378     191

  Fragments arranged by size: 

                 555     378     292     191      94      70      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     942    1590 
   Size:        942     648
 
EcoO109I rG'GnC_Cy

Cuts at:      0     654    1371    1590 
   Size:        654     717     219

  Fragments arranged by size: 

                 717     654     219
 
EcoRI G'AATT_C

Cuts at:      0     448    1590 
   Size:        448    1142
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     705     897    1590 
   Size:        705     192     693

  Fragments arranged by size: 

                 705     693     192
 
HaeII r_GCGC'y

Cuts at:      0     316     498     783    1027    1590 
   Size:        316     182     285     244     563

  Fragments arranged by size: 

                 563     316     285     244     182
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     318     350     610     642     952     984 
   Size:        110      32     176      32     260      32     310      32

Cuts at:    984    1590 
   Size:        606

  Fragments arranged by size: 

                 606     310     260     176     110      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1480    1590 
   Size:       1480     110
 
MfeI C'AATT_G

Cuts at:      0     211    1590 
   Size:        211    1379
 
MluI A'CGCG_T

Cuts at:      0     623    1590 
   Size:        623     967
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     565    1590 
   Size:         96     469    1025

  Fragments arranged by size: 

                1025     469      96
 
MscI TGG'CCA

Cuts at:      0    1367    1590 
   Size:       1367     223
 
MslI CAynn'nnrTG

Cuts at:      0     110    1590 
   Size:        110    1480
 
MspA1I CmG'CkG

Cuts at:      0    1158    1590 
   Size:       1158     432
 
NaeI GCC'GGC

Cuts at:      0    1520    1590 
   Size:       1520      70
 
NcoI C'CATG_G

Cuts at:      0    1362    1590 
   Size:       1362     228
 
NgoMIV G'CCGG_C

Cuts at:      0    1518    1590 
   Size:       1518      72
 
PfoI T'CCnGG_A

Cuts at:      0     943    1037    1590 
   Size:        943      94     553

  Fragments arranged by size: 

                 943     553      94
 
PmlI CAC'GTG

Cuts at:      0     816    1590 
   Size:        816     774
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     512     544    1590 
   Size:        512      32    1046

  Fragments arranged by size: 

                1046     512      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     654    1590 
   Size:        654     936
 
PstI C_TGCA'G

Cuts at:      0    1091    1298    1590 
   Size:       1091     207     292

  Fragments arranged by size: 

                1091     292     207
 
SacI G_AGCT'C

Cuts at:      0     336    1037    1139    1590 
   Size:        336     701     102     451

  Fragments arranged by size: 

                 701     451     336     102
 
SacII CC_GC'GG

Cuts at:      0    1159    1590 
   Size:       1159     431
 
SalI G'TCGA_C

Cuts at:      0    1478    1590 
   Size:       1478     112
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1127    1590 
   Size:       1127     463
 
SbfI CC_TGCA'GG

Cuts at:      0    1298    1590 
   Size:       1298     292
 
SfcI C'TryA_G

Cuts at:      0    1087    1294    1426    1590 
   Size:       1087     207     132     164

  Fragments arranged by size: 

                1087     207     164     132
 
SmlI C'TyrA_G

Cuts at:      0     153     248     548     871    1590 
   Size:        153      95     300     323     719

  Fragments arranged by size: 

                 719     323     300     153      95
 
StyI C'CwwG_G

Cuts at:      0     633    1362    1590 
   Size:        633     729     228

  Fragments arranged by size: 

                 729     633     228
 
XmnI GAAnn'nnTTC

Cuts at:      0    1503    1590 
   Size:       1503      87
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AgeI     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI    BanII     BbsI    BbvCI    BglII     BlpI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BsrBI    BsrFI    BstYI     BtgI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     PfoI     PmlI     PpiI    PpuMI     PstI
     SacI    SacII     SalI     SapI     SbfI     SfcI     SmlI     StyI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI     BmrI     BmtI    BsaBI
    BsaHI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     NarI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SanDI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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