Restriction map for pF1KSDA1663
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BanII       BbrPI       BcgI        BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmAI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        Eam1105I    Eco47III    EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MroI        MseI        MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TaqI        TfiI        Tru9I       Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        131  GGTACC               G^GTACC
AcsI          553  AAATTT               [GA]^AATT[CT]
AlwI          283  GATCC                GGATC(4/5)
AocI          259  CCTGAGG              CC^T[ACGT]AGG
ApoI          553  AAATTT               [GA]^AATT[CT]
Asp718I       131  GGTACC               G^GTACC
AvaII         192  GGACC                G^G[AT]CC
BbeI           65  GGCGCC               GGCGC^C
BbsI          945  GAAGAC               GAAGAC(2/6)
BbvCI         966  CCTCAGC              CCTCAGC(-5/-2)
BciVI         993  GTATCC               GTATCC(6/5)
BglI          733  GCCAACCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          511  GCTCAGC              GC^T[ACGT]AGC
BpmI          940  CTGGAG               CTGGAG(16/14)
Bpu1102I      511  GCTCAGC              GC^T[ACGT]AGC
BpuAI         945  GAAGAC               GAAGAC(2/6)
BsiEI         821  CGGCCG               CG[GA][CT]^CG
BsmFI         750  GTCCC                GGGAC(10/14)
BsmI          711  GCATTC               GAATGC(1/-1)
BspMI         877  ACCTGC               ACCTGC(4/8)
BstXI         906  CCAGGTATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        259  CCTGAGG              CC^T[ACGT]AGG
CelII         511  GCTCAGC              GC^T[ACGT]AGC
CfoI           66  GCGC                 GCG^C
EagI          821  CGGCCG               C^GGCCG
EclXI         821  CGGCCG               C^GGCCG
Eco52I        821  CGGCCG               C^GGCCG
Eco81I        259  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       770  CCAAGG               C^C[AT][AT]GG
EcoT22I       709  ATGCAT               ATGCA^T
HaeII          65  GGCGCC               [GA]GCGC^[CT]
HgaI          406  GACGC                GACGC(5/10)
HhaI           66  GCGC                 GCG^C
HinP1I         66  GCGC                 G^CGC
KasI           65  GGCGCC               G^GCGCC
KpnI          131  GGTACC               GGTAC^C
MaeIII        480  GTCAC                ^GT[ACGT]AC
MslI          564  CACTAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI           65  GGCGCC               GG^CGCC
NsiI          709  ATGCAT               ATGCA^T
SfaNI         817  GATGC                GCATC(5/9)
SfiI          732  GGCCAACCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           65  GGCGCC               GGC^GCC
StuI          963  AGGCCT               AGG^CCT
StyI          770  CCAAGG               C^C[AT][AT]GG
Tsp45I        480  GTCAC                ^GT[GC]AC
Tsp509I       554  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI           65  GGCGCC               G[GA]^CG[CT]C
              406  GACGCC              
BfaI            7  CTAG                 C^TAG
               94  CTAG                
BmyI          362  GTGCCC               G[AGT]GC[ACT]^C
              421  GTGCCC              
BsaHI          65  GGCGCC               G[GA]^CG[CT]C
              406  GACGCC              
BseRI          88  GAGGAG               GAGGAG(10/8)
              262  GAGGAG              
Bsp1286I      362  GTGCCC               G[AGT]GC[ACT]^C
              421  GTGCCC              
Bsp1407I       47  TGTACA               T^GTACA
              782  TGTACA              
BsrGI          47  TGTACA               T^GTACA
              782  TGTACA              
BsrI          128  ACTGG                ACTGG(1/-1)
              477  CCAGT               
BstF5I        467  CATCC                GGATG(2/0)
              342  GGATG               
DsaI          197  CCGTGG               C^C[GA][CT]GG
             1016  CCGTGG              
EarI          808  CTCTTC               CTCTTC(1/4)
              934  CTCTTC              
FokI          467  CATCC                GGATG(9/13)
              342  GGATG               
Hin1I          65  GGCGCC               G[GA]^CG[CT]C
              406  GACGCC              
Ksp632I       808  CTCTTC               CTCTTC(1/4)
              934  CTCTTC              
MaeI            7  CTAG                 C^TAG
               94  CTAG                
MspA1I        165  CCGCTG               C[AC]G^C[GT]G
              837  CAGCGG              
PflMI         466  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1011  CCAGCCCGTGG         
SspBI          47  TGTACA               T^GTACA
              782  TGTACA              
Van91I        466  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1011  CCAGCCCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BanI        
BbvI        BcnI        BsaJI       BsiYI       BslI        
BsrBI       BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HpaII       HphI        
ItaI        MboI        MboII       MluNI       MnlI        
MscI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com