Restriction map for pF1KSDA1663
 (Linear) (Six-base) MAPSORT of: KIAA1663.seq  Check: 7882  from: 1  to: 1032



KIAA1663.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     886    1032 
   Size:        886     146
 
Acc65I G'GTAC_C

Cuts at:      0     131    1032 
   Size:        131     901
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1032 
   Size:        703     329
 
ApoI r'AATT_y

Cuts at:      0     553    1032 
   Size:        553     479
 
BanI G'GyrC_C

Cuts at:      0      65     131     361     841     874     922    1032 
   Size:         65      66     230     480      33      48     110

  Fragments arranged by size: 

                 480     230     110      66      65      48      33
 
BbeI G_GCGC'C

Cuts at:      0      69    1032 
   Size:         69     963
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     952    1032 
   Size:        952      80
 
BbvCI CC'TCA_GC

Cuts at:      0     967    1032 
   Size:        967      65
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1004    1032 
   Size:       1004      28
 
BfrBI ATG'CAT

Cuts at:      0     711    1032 
   Size:        711     321
 
BglI GCCn_nnn'nGGC

Cuts at:      0     739    1032 
   Size:        739     293
 
BlpI GC'TnA_GC

Cuts at:      0     512    1032 
   Size:        512     520
 
Bme1580I G_kGCm'C

Cuts at:      0     366     425    1032 
   Size:        366      59     607

  Fragments arranged by size: 

                 607     366      59
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     716    1032 
   Size:        716     316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1032 
   Size:        961      71
 
Bpu10I CC'TnA_GC

Cuts at:      0     967    1032 
   Size:        967      65
 
BsaHI Gr'CG_yC

Cuts at:      0      66     407    1032 
   Size:         66     341     625

  Fragments arranged by size: 

                 625     341      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796    1032 
   Size:        766      30     236

  Fragments arranged by size: 

                 766     236      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     103     277    1032 
   Size:        103     174     755

  Fragments arranged by size: 

                 755     174     103
 
BseYI C'CCAG_C

Cuts at:      0     484     752     802     997    1032 
   Size:        484     268      50     195      35

  Fragments arranged by size: 

                 484     268     195      50      35
 
BsiEI CG_ry'CG

Cuts at:      0     824    1032 
   Size:        824     208
 
BsmI GAATG_Cn'

Cuts at:      0     711    1032 
   Size:        711     321
 
Bsp1286I G_dGCh'C

Cuts at:      0     366     425    1032 
   Size:        366      59     607

  Fragments arranged by size: 

                 607     366      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     886    1032 
   Size:        886     146
 
BsrBI CCG'CTC

Cuts at:      0      99     506     511     662    1032 
   Size:         99     407       5     151     370

  Fragments arranged by size: 

                 407     370     151      99       5
 
BsrGI T'GTAC_A

Cuts at:      0      47     782    1032 
   Size:         47     735     250

  Fragments arranged by size: 

                 735     250      47
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     913    1032 
   Size:        913     119
 
Bsu36I CC'TnA_GG

Cuts at:      0     260    1032 
   Size:        260     772
 
BtgI C'CryG_G

Cuts at:      0     197    1016    1032 
   Size:        197     819      16

  Fragments arranged by size: 

                 819     197      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     446    1032 
   Size:        446     586
 
BtsI GCAGTG_nn'

Cuts at:      0     621     870    1032 
   Size:        621     249     162

  Fragments arranged by size: 

                 621     249     162
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     204     239     537     572     774     809    1032 
   Size:        204      35     298      35     202      35     223

  Fragments arranged by size: 

                 298     223     204     202      35      35      35
 
EaeI y'GGCC_r

Cuts at:      0     209     474     627     731     821     828    1025    1032 
   Size:        209     265     153     104      90       7     197       7

  Fragments arranged by size: 

                 265     209     197     153     104      90       7       7
            
 
EagI C'GGCC_G

Cuts at:      0     821    1032 
   Size:        821     211
 
EarI CTCTTCn'nnn_

Cuts at:      0     814     940    1032 
   Size:        814     126      92

  Fragments arranged by size: 

                 814     126      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703     961    1032 
   Size:        703     258      71

  Fragments arranged by size: 

                 703     258      71
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     411     969    1032 
   Size:        411     558      63

  Fragments arranged by size: 

                 558     411      63
 
HaeII r_GCGC'y

Cuts at:      0      69    1032 
   Size:         69     963
 
KasI G'GCGC_C

Cuts at:      0      65    1032 
   Size:         65     967
 
KpnI G_GTAC'C

Cuts at:      0     135    1032 
   Size:        135     897
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     476    1032 
   Size:        476     556
 
MscI TGG'CCA

Cuts at:      0     476     629     830    1027    1032 
   Size:        476     153     201     197       5

  Fragments arranged by size: 

                 476     201     197     153       5
 
MslI CAynn'nnrTG

Cuts at:      0     568    1032 
   Size:        568     464
 
MspA1I CmG'CkG

Cuts at:      0     167     839    1032 
   Size:        167     672     193

  Fragments arranged by size: 

                 672     193     167
 
NarI GG'CG_CC

Cuts at:      0      66    1032 
   Size:         66     966
 
NsiI A_TGCA'T

Cuts at:      0     713    1032 
   Size:        713     319
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     472    1017    1032 
   Size:        472     545      15

  Fragments arranged by size: 

                 545     472      15
 
PfoI T'CCnGG_A

Cuts at:      0     938    1032 
   Size:        938      94
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     739    1032 
   Size:        739     293
 
SfoI GGC'GCC

Cuts at:      0      67    1032 
   Size:         67     965
 
StuI AGG'CCT

Cuts at:      0     965    1032 
   Size:        965      67
 
StyI C'CwwG_G

Cuts at:      0     770    1032 
   Size:        770     262
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     498     681     777     816    1032 
   Size:        498     183      96      39     216

  Fragments arranged by size: 

                 498     216     183      96      39
 
TatI w'GTAC_w

Cuts at:      0      47     782    1032 
   Size:         47     735     250

  Fragments arranged by size: 

                 735     250      47
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     ApoI     BanI     BbeI     BbsI    BbvCI
    BciVI    BfrBI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BsaHI    BsaXI    BseRI    BseYI    BsiEI     BsmI Bsp1286I    BspMI
    BsrBI    BsrGI    BstXI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     EaeI     EagI     EarI  Eco57MI     FalI    HaeII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NsiI    PflMI     PfoI
     SfiI     SfoI     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BcgI     BclI
    BglII    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
     ClaI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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