Restriction map for pF1KSDA1587
 (Linear) (Six-base) MAPSORT of: KIAA1587.seq  Check: 9323  from: 1  to: 2871



KIAA1587.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AatII G_ACGT'C

Cuts at:      0     134    2871 
   Size:        134    2737
 
AccI GT'mk_AC

Cuts at:      0    2480    2871 
   Size:       2480     391
 
Acc65I G'GTAC_C

Cuts at:      0     474    2160    2871 
   Size:        474    1686     711

  Fragments arranged by size: 

                1686     711     474
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1474    2026    2850    2871 
   Size:       1474     552     824      21

  Fragments arranged by size: 

                1474     824     552      21
 
AfeI AGC'GCT

Cuts at:      0    2644    2871 
   Size:       2644     227
 
AleI CACnn'nnGTG

Cuts at:      0     235     307     379     451     523     559     595     631 
   Size:        235      72      72      72      72      36      36      36

Cuts at:    631     667     703     739     775     811     883     919     955 
   Size:         36      36      36      36      36      72      36      36

Cuts at:    955     991    1027    1099    1135    1171    2871 
   Size:         36      36      72      36      36    1700

  Fragments arranged by size: 

                1700     235      72      72      72      72      72      72
                  36      36      36      36      36      36      36      36
                  36      36      36      36      36      36
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     253     325     469     505     541     613     649     685 
   Size:        253      72     144      36      36      72      36      36

Cuts at:    685     721     757     793     829     901     937     947     973 
   Size:         36      36      36      36      72      36      10      26

Cuts at:    973    1009    1045    1117    1189    2871 
   Size:         36      36      72      72    1682

  Fragments arranged by size: 

                1682     253     144      72      72      72      72      72
                  36      36      36      36      36      36      36      36
                  36      36      36      26      10
 
AlwNI CAG_nnn'CTG

Cuts at:      0      71    1339    1503    1930    2871 
   Size:         71    1268     164     427     941

  Fragments arranged by size: 

                1268     941     427     164      71
 
ApaI G_GGCC'C

Cuts at:      0     190     226    1234    1969    2871 
   Size:        190      36    1008     735     902

  Fragments arranged by size: 

                1008     902     735     190      36
 
ApaLI G'TGCA_C

Cuts at:      0     372    2871 
   Size:        372    2499
 
ApoI r'AATT_y

Cuts at:      0      18    1372    2000    2309    2871 
   Size:         18    1354     628     309     562

  Fragments arranged by size: 

                1354     628     562     309      18
 
AvaI C'yCGr_G

Cuts at:      0    1233    1436    2871 
   Size:       1233     203    1435

  Fragments arranged by size: 

                1435    1233     203
 
AvrII C'CTAG_G

Cuts at:      0    1306    2871 
   Size:       1306    1565
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2622    2655    2871 
   Size:       2622      33     216

  Fragments arranged by size: 

                2622     216      33
 
BanI G'GyrC_C

Cuts at:      0     301     474     564     625     852    2160    2871 
   Size:        301     173      90      61     227    1308     711

  Fragments arranged by size: 

                1308     711     301     227     173      90      61
 
BanII G_rGCy'C

Cuts at:      0     190     226    1234    1402    1726    1969    2715    2871 
   Size:        190      36    1008     168     324     243     746     156

  Fragments arranged by size: 

                1008     746     324     243     190     168     156      36
            
 
BbvCI CC'TCA_GC

Cuts at:      0     218    2099    2871 
   Size:        218    1881     772

  Fragments arranged by size: 

                1881     772     218
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1798    2871 
   Size:       1798    1073
 
BlpI GC'TnA_GC

Cuts at:      0    1487    2871 
   Size:       1487    1384
 
Bme1580I G_kGCm'C

Cuts at:      0     190     226     376     569     857     890    1234    1703 
   Size:        190      36     150     193     288      33     344     469

Cuts at:   1703    1969    2843    2871 
   Size:        266     874      28

  Fragments arranged by size: 

                 874     469     344     288     266     193     190     150
                  36      33      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     317    1699    2871 
   Size:        317    1382    1172

  Fragments arranged by size: 

                1382    1172     317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     399    1639    1842    2871 
   Size:        148     251    1240     203    1029

  Fragments arranged by size: 

                1240    1029     251     203     148
 
Bpu10I CC'TnA_GC

Cuts at:      0     218     334     658     730     910    2099    2524    2589 
   Size:        218     116     324      72     180    1189     425      65

Cuts at:   2589    2871 
   Size:        282

  Fragments arranged by size: 

                1189     425     324     282     218     180     116      72
                  65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246    2655    2871 
   Size:        246    2409     216

  Fragments arranged by size: 

                2409     246     216
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109    2871 
   Size:        109    2762
 
BsaHI Gr'CG_yC

Cuts at:      0     131    2871 
   Size:        131    2740
 
BsaWI w'CCGG_w

Cuts at:      0    1268    2871 
   Size:       1268    1603
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1939    1969    2871 
   Size:       1939      30     902

  Fragments arranged by size: 

                1939     902      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1027    2485    2871 
   Size:       1027    1458     386

  Fragments arranged by size: 

                1458    1027     386
 
BseYI C'CCAG_C

Cuts at:      0      71     145    2602    2871 
   Size:         71      74    2457     269

  Fragments arranged by size: 

                2457     269      74      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     943    1195    1376    1855    2278    2355    2871 
   Size:        691     252     252     181     479     423      77     516

  Fragments arranged by size: 

                 691     516     479     423     252     252     181      77
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     376     484     520     664     700     772     808     916 
   Size:        376     108      36     144      36      72      36     108

Cuts at:    916     952     988    1024    1096    1240    2871 
   Size:         36      36      36      72     144    1631

  Fragments arranged by size: 

                1631     376     144     144     108     108      72      72
                  36      36      36      36      36      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     190     226     376     484     520     569     664     700 
   Size:        190      36     150     108      36      49      95      36

Cuts at:    700     772     808     857     890     916     952     988    1024 
   Size:         72      36      49      33      26      36      36      36

Cuts at:   1024    1096    1234    1240    1402    1703    1726    1969    2715 
   Size:         72     138       6     162     301      23     243     746

Cuts at:   2715    2843    2871 
   Size:        128      28

  Fragments arranged by size: 

                 746     301     243     190     162     150     138     128
                 108      95      72      72      49      49      36      36
                  36      36      36      36      36      33      28      26
                  23       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2508    2632    2871 
   Size:       2508     124     239

  Fragments arranged by size: 

                2508     239     124
 
BsrBI CCG'CTC

Cuts at:      0     639    2871 
   Size:        639    2232
 
BsrDI GCAATG_nn'

Cuts at:      0    2813    2871 
   Size:       2813      58
 
BsrFI r'CCGG_y

Cuts at:      0     780    2871 
   Size:        780    2091
 
BsrGI T'GTAC_A

Cuts at:      0    2441    2871 
   Size:       2441     430
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1503    2871 
   Size:       1503    1368
 
BstEII G'GTnAC_C

Cuts at:      0    1311    2871 
   Size:       1311    1560
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2216    2392    2783    2871 
   Size:       2216     176     391      88

  Fragments arranged by size: 

                2216     391     176      88
 
BstYI r'GATC_y

Cuts at:      0     158    1972    2034    2871 
   Size:        158    1814      62     837

  Fragments arranged by size: 

                1814     837     158      62
 
Bsu36I CC'TnA_GG

Cuts at:      0    1349    1658    2871 
   Size:       1349     309    1213

  Fragments arranged by size: 

                1349    1213     309
 
BtgI C'CryG_G

Cuts at:      0     560     861    1325    2871 
   Size:        560     301     464    1546

  Fragments arranged by size: 

                1546     560     464     301
 
BtsI GCAGTG_nn'

Cuts at:      0     929    2871 
   Size:        929    1942
 
DraIII CAC_nnn'GTG

Cuts at:      0     613    2871 
   Size:        613    2258
 
EaeI y'GGCC_r

Cuts at:      0     782    2047    2325    2753    2785    2871 
   Size:        782    1265     278     428      32      86

  Fragments arranged by size: 

                1265     782     428     278      86      32
 
EarI CTCTTCn'nnn_

Cuts at:      0    1712    2871 
   Size:       1712    1159
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     399    1474    1639    1842    2026    2850    2871 
   Size:        148     251    1075     165     203     184     824      21

  Fragments arranged by size: 

                1075     824     251     203     184     165     148      21
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     142     168     186     222     295     330     654 
   Size:         87      55      26      18      36      73      35     324

Cuts at:    654     726     906    1230    1345    1363    1380    1693    1965 
   Size:         72     180     324     115      18      17     313     272

Cuts at:   1965    2132    2520    2704    2871 
   Size:        167     388     184     167

  Fragments arranged by size: 

                 388     324     324     313     272     184     180     167
                 167     115      87      73      72      55      36      35
                  26      18      18      17
 
EcoRI G'AATT_C

Cuts at:      0      18    1372    2309    2871 
   Size:         18    1354     937     562

  Fragments arranged by size: 

                1354     937     562      18
 
FspI TGC'GCA

Cuts at:      0      59    2871 
   Size:         59    2812
 
HaeII r_GCGC'y

Cuts at:      0     269    2646    2871 
   Size:        269    2377     225

  Fragments arranged by size: 

                2377     269     225
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     827     859    2149    2181    2871 
   Size:        827      32    1290      32     690

  Fragments arranged by size: 

                1290     827     690      32      32
 
HindIII A'AGCT_T

Cuts at:      0    2526    2871 
   Size:       2526     345
 
KpnI G_GTAC'C

Cuts at:      0     478    2164    2871 
   Size:        478    1686     707

  Fragments arranged by size: 

                1686     707     478
 
MscI TGG'CCA

Cuts at:      0    2049    2755    2787    2871 
   Size:       2049     706      32      84

  Fragments arranged by size: 

                2049     706      84      32
 
MslI CAynn'nnrTG

Cuts at:      0     235     307     379     451     523     559     595     631 
   Size:        235      72      72      72      72      36      36      36

Cuts at:    631     667     703     739     775     811     883     919     955 
   Size:         36      36      36      36      36      72      36      36

Cuts at:    955     991    1027    1099    1135    1171    1993    2214    2390 
   Size:         36      36      72      36      36     822     221     176

Cuts at:   2390    2871 
   Size:        481

  Fragments arranged by size: 

                 822     481     235     221     176      72      72      72
                  72      72      72      36      36      36      36      36
                  36      36      36      36      36      36      36      36
                  36
 
MspA1I CmG'CkG

Cuts at:      0      71     112     217     863    1839    2871 
   Size:         71      41     105     646     976    1032

  Fragments arranged by size: 

                1032     976     646     105      71      41
 
NaeI GCC'GGC

Cuts at:      0     782    2871 
   Size:        782    2089
 
NgoMIV G'CCGG_C

Cuts at:      0     780    2871 
   Size:        780    2091
 
NspI r_CATG'y

Cuts at:      0    2617    2871 
   Size:       2617     254
 
PasI CC'CwG_GG

Cuts at:      0     138    2871 
   Size:        138    2733
 
PfoI T'CCnGG_A

Cuts at:      0    1535    2871 
   Size:       1535    1336
 
PpuMI rG'GwC_Cy

Cuts at:      0     142     295    1345    1380    1693    2520    2704    2871 
   Size:        142     153    1050      35     313     827     184     167

  Fragments arranged by size: 

                1050     827     313     184     167     153     142      35
            
 
PshAI GACnn'nnGTC

Cuts at:      0    1881    2871 
   Size:       1881     990
 
PspOMI G'GGCC_C

Cuts at:      0     186     222    1230    1965    2871 
   Size:        186      36    1008     735     906

  Fragments arranged by size: 

                1008     906     735     186      36
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2393    2425    2871 
   Size:       2393      32     446

  Fragments arranged by size: 

                2393     446      32
 
PstI C_TGCA'G

Cuts at:      0    2157    2568    2629    2871 
   Size:       2157     411      61     242

  Fragments arranged by size: 

                2157     411     242      61
 
PvuII CAG'CTG

Cuts at:      0      71    2871 
   Size:         71    2800
 
RsrII CG'GwC_CG

Cuts at:      0    1195    2871 
   Size:       1195    1676
 
SacII CC_GC'GG

Cuts at:      0     864    2871 
   Size:        864    2007
 
SanDI GG'GwC_CC

Cuts at:      0     142    2871 
   Size:        142    2729
 
SexAI A'CCwGG_T

Cuts at:      0     609    2871 
   Size:        609    2262
 
SfcI C'TryA_G

Cuts at:      0    1367    1917    2153    2564    2625    2871 
   Size:       1367     550     236     411      61     246

  Fragments arranged by size: 

                1367     550     411     246     236      61
 
SmaI CCC'GGG

Cuts at:      0    1438    2871 
   Size:       1438    1433
 
SmlI C'TyrA_G

Cuts at:      0     261    2634    2871 
   Size:        261    2373     237

  Fragments arranged by size: 

                2373     261     237
 
SpeI A'CTAG_T

Cuts at:      0    2403    2871 
   Size:       2403     468
 
SspI AAT'ATT

Cuts at:      0    1772    2871 
   Size:       1772    1099
 
StuI AGG'CCT

Cuts at:      0     259    1321    2496    2871 
   Size:        259    1062    1175     375

  Fragments arranged by size: 

                1175    1062     375     259
 
StyI C'CwwG_G

Cuts at:      0     848    1306    1316    1793    2871 
   Size:        848     458      10     477    1078

  Fragments arranged by size: 

                1078     848     477     458      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     708     762     780     816    2871 
   Size:        708      54      18      36    2055

  Fragments arranged by size: 

                2055     708      54      36      18
 
TatI w'GTAC_w

Cuts at:      0    2315    2441    2795    2871 
   Size:       2315     126     354      76

  Fragments arranged by size: 

                2315     354     126      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1193    1225    2871 
   Size:       1193      32    1646

  Fragments arranged by size: 

                1646    1193      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1323    1416    2871 
   Size:       1323      93    1455

  Fragments arranged by size: 

                1455    1323      93
 
XmaI C'CCGG_G

Cuts at:      0    1436    2871 
   Size:       1436    1435
 
ZraI GAC'GTC

Cuts at:      0     132    2871 
   Size:        132    2739
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII     BaeI     BanI    BanII
    BbvCI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I  HindIII     KpnI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI
    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII    RsrII    SacII
    SanDI    SexAI     SfcI     SmaI     SmlI     SpeI     SspI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII   AflIII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI    BamHI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KasI     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI     PsiI    PspXI     PvuI     SacI     SalI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SphI     SrfI
     SwaI  Tth111I     XbaI     XhoI     XmnI
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