Restriction map for pF1KSDA1585
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BanI        BanII       BbeI        BbrPI       BbsI        
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          36  CGCG                 CG^CG
AcsI         1136  AAATTT               [GA]^AATT[CT]
ApoI         1136  AAATTT               [GA]^AATT[CT]
Asp700I      1306  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          49  GTGCTC               G[AT]GC[AT]^C
BalI         1508  TGGCCA               TGG^CCA
BamHI        1243  GGATCC               G^GATCC
BbvCI         543  CCTCAGC              CCTCAGC(-5/-2)
BciVI         892  GTATCC               GTATCC(6/5)
BcnI           64  CCCGG                CC^[GC]GG
BmyI           49  GTGCTC               G[AGT]GC[ACT]^C
BsaBI        1025  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           68  GAGACC               GGTCTC(1/5)
BseRI         421  GAGGAG               GAGGAG(10/8)
BsgI          962  CTGCAC               GTGCAG(16/14)
BsiHKAI        49  GTGCTC               G[AT]GC[AT]^C
BsmI           12  GAATGC               GAATGC(1/-1)
Bsp1286I       49  GTGCTC               G[AGT]GC[ACT]^C
BspHI        1233  TCATGA               T^CATGA
BspMI         181  ACCTGC               ACCTGC(4/8)
BstAPI       1460  GCAAAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          36  CGCG                 CG^CG
DraII        1648  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI         1508  TGGCCA               [CT]^GGCC[GA]
EarI         1142  CTCTTC               CTCTTC(1/4)
EcoO109I     1648  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV         754  GATATC               GAT^ATC
HapII          65  CCGG                 C^CGG
HpaII          65  CCGG                 C^CGG
HphI          631  TCACC                GGTGA(8/7)
Ksp632I      1142  CTCTTC               CTCTTC(1/4)
MamI         1025  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1508  TGGCCA               TGG^CCA
MscI         1508  TGGCCA               TGG^CCA
MspI           65  CCGG                 C^CGG
MvnI           36  CGCG                 CG^CG
NciI           64  CCCGG                CC^[GC]GG
NspI         1423  ACATGC               [GA]CATG^[CT]
PpuMI        1648  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         1233  TCATGA               T^CATGA
ScaI          816  AGTACT               AGT^ACT
SmlI          509  CTCAAG               C^T[CT][GA]AG
SspI          622  AATATT               AAT^ATT
StuI         1553  AGGCCT               AGG^CCT
TaqI         1724  TCGA                 T^CGA
TthHB8I      1724  TCGA                 T^CGA
XcmI         1568  CCAGGCTCACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1306  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaII         901  GGACC                G^G[AT]CC
             1649  GGTCC               
BpmI          785  CTGGAG               CTGGAG(16/14)
             1528  CTGGAG              
BsmFI         120  GTCCC                GGGAC(10/14)
             1650  GTCCC               
Bsp1407I      982  TGTACA               T^GTACA
             1355  TGTACA              
BsrDI         549  CATTGC               GCAATG(2/0)
             1599  CATTGC              
BsrGI         982  TGTACA               T^GTACA
             1355  TGTACA              
EcoT14I        74  CCAAGG               C^C[AT][AT]GG
             1556  CCTTGG              
HincII        367  GTTGAC               GT[CT]^[GA]AC
              670  GTTGAC              
HindII        367  GTTGAC               GT[CT]^[GA]AC
              670  GTTGAC              
PleI          790  GACTC                GAGTC(4/5)
             1230  GAGTC               
PstI          759  CTGCAG               CTGCA^G
              910  CTGCAG              
SfaNI         290  GCATC                GCATC(5/9)
             1102  GCATC               
SspBI         982  TGTACA               T^GTACA
             1355  TGTACA              
StyI           74  CCAAGG               C^C[AT][AT]GG
             1556  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       
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