Restriction map for pF1KSDA1499
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AccII       AclI        AcsI        AflII       AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BbeI        BbsI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         270  GACGTC               GACGT^C
AccI         1285  GTAGAC               GT^[AC][GT]AC
AccIII        503  TCCGGA               T^CCGGA
Alw44I        415  GTGCAC               G^TGCAC
ApaI          250  GGGCCC               GGGCC^C
ApaLI         415  GTGCAC               G^TGCAC
AspI          940  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1463  TGGCCA               TGG^CCA
BbrPI        1835  CACGTG               CAC^GTG
BbvCI         339  CCTCAGC              CCTCAGC(-5/-2)
BcgI          888  CGATGAAATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1503  GGATAC               GTATCC(6/5)
BclI          330  TGATCA               T^GATCA
BglI          389  GCCGCCACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          374  GAGACC               GGTCTC(1/5)
BseAI         503  TCCGGA               T^CCGGA
BspEI         503  TCCGGA               T^CCGGA
BspMI        1785  GCAGGT               ACCTGC(4/8)
BsrDI        1798  GCAATG               GCAATG(2/0)
BstAPI       1254  GCAGTACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1539  GGTCACC              G^GT[ACGT]ACC
BstPI        1539  GGTCACC              G^GT[ACGT]ACC
DraII         250  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1696  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1129  GACAACCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         912  CCTGCGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      250  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1539  GGTCACC              G^GT[ACGT]ACC
FbaI          330  TGATCA               T^GATCA
MluNI        1463  TGGCCA               TGG^CCA
MroI          503  TCCGGA               T^CCGGA
MscI         1463  TGGCCA               TGG^CCA
MseI          569  TTAA                 T^TAA
MslI          380  CACAGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1064  GCCGGC               GCC^GGC
NgoMIV       1064  GCCGGC               G^CCGGC
PflFI         940  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1455  CCATAGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1835  CACGTG               CAC^GTG
PmlI         1835  CACGTG               CAC^GTG
SacI         1612  GAGCTC               GAGCT^C
SexAI        1154  ACCAGGT              A^CC[AT]GGT
Tru9I         569  TTAA                 T^TAA
Tsp45I       1540  GTCAC                ^GT[GC]AC
Tth111I       940  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1455  CCATAGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          429  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        967  GGTACC               G^GTACC
             1151  GGTACC              
AcyI          270  GACGTC               G[GA]^CG[CT]C
             1213  GACGCC              
AflIII        482  ACATGT               A^C[GA][CT]GT
             1058  ACATGT              
Asp718I       967  GGTACC               G^GTACC
             1151  GGTACC              
AvaI          237  CTCGAG               C^[CT]CG[GA]G
              463  CTCGAG              
BanII         250  GGGCCC               G[GA]GC[CT]^C
             1612  GAGCTC              
BfaI          430  CTAG                 C^TAG
             1244  CTAG                
BsaAI        1258  TACGTG               [CT]AC^GT[GA]
             1835  CACGTG              
BsaHI         270  GACGTC               G[GA]^CG[CT]C
             1213  GACGCC              
BsaWI         157  ACCGGA               [AT]^CCGG[AT]
              503  TCCGGA              
BseRI         174  CTCCTC               GAGGAG(10/8)
             1609  GAGGAG              
BsgI         1729  CTGCAC               GTGCAG(16/14)
             1665  GTGCAG              
BsmBI         945  CGTCTC               CGTCTC(1/5)
              268  GAGACG              
BsmFI          30  GTCCC                GGGAC(10/14)
              424  GTCCC               
BsoBI         237  CTCGAG               C^[CT]CG[GA]G
              463  CTCGAG              
BspLU11I      482  ACATGT               A^CATGT
             1058  ACATGT              
BsrFI        1064  GCCGGC               [GA]^CCGG[CT]
             1746  GCCGGT              
CfoI          302  GCGC                 GCG^C
              632  GCGC                
Cfr10I       1064  GCCGGC               [GA]^CCGG[CT]
             1746  GCCGGT              
EarI         1015  GAAGAG               CTCTTC(1/4)
             1629  GAAGAG              
HaeII         301  GGCGCT               [GA]GCGC^[CT]
              631  AGCGCC              
HgaI         1213  GACGC                GACGC(5/10)
              422  GCGTC               
HhaI          302  GCGC                 GCG^C
              632  GCGC                
Hin1I         270  GACGTC               G[GA]^CG[CT]C
             1213  GACGCC              
HinP1I        302  GCGC                 G^CGC
              632  GCGC                
KpnI          967  GGTACC               GGTAC^C
             1151  GGTACC              
Ksp632I      1015  GAAGAG               CTCTTC(1/4)
             1629  GAAGAG              
MaeI          430  CTAG                 C^TAG
             1244  CTAG                
MaeIII       1102  GTTAC                ^GT[ACGT]AC
             1540  GTCAC               
PaeR7I        237  CTCGAG               C^TCGAG
              463  CTCGAG              
PleI         1815  GACTC                GAGTC(4/5)
             1240  GAGTC               
TfiI          687  GAATC                G^A[AT]TC
             1761  GATTC               
Tsp509I        18  AATT                 ^AATT
              894  AATT                
XhoI          237  CTCGAG               C^TCGAG
              463  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BamHI       BanI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeII       MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TseI        TspRI       TthHB8I     XcmI        XhoII       
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