Restriction map for pF1KSDA1458
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BbrPI       
BcgI        BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      EcoO109I    EcoO65I     EcoRI       EcoRV       
FseI        FspI        HindIII     KpnI        KspI        
MamI        MluI        MluNI       MroI        MscI        
NdeI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspI        StuI        SwaI        Van91I      XbaI        
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1003  GTATAC               GT^[AC][GT]AC
AlwNI          52  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1345  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1558  AGCGCT               AGC^GCT
AspHI        1521  GAGCAC               G[AT]GC[AT]^C
AspI         1232  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          30  GGACC                G^G[AT]CC
BamHI         799  GGATCC               G^GATCC
BanI          118  GGCGCC               G^G[CT][GA]CC
BbeI          118  GGCGCC               GGCGC^C
BbsI         1238  GTCTTC               GAAGAC(2/6)
BpuAI        1238  GTCTTC               GAAGAC(2/6)
BsiEI        1051  CGACCG               CG[GA][CT]^CG
BsiHKAI      1521  GAGCAC               G[AT]GC[AT]^C
Bsp1407I      831  TGTACA               T^GTACA
BspMI        1575  ACCTGC               ACCTGC(4/8)
BsrBI          99  CCGCTC               CCGCTC(-3/-3)
BsrGI         831  TGTACA               T^GTACA
Bst1107I     1003  GTATAC               GTA^TAC
BstZ17I      1003  GTATAC               GTA^TAC
Bsu36I       1345  CCTCAGG              CC^T[ACGT]AGG
DsaI         1440  CCATGG               C^C[GA][CT]GG
Eco47III     1558  AGCGCT               AGC^GCT
Eco81I       1345  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1363  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       991  ATGCAT               ATGCA^T
KasI          118  GGCGCC               G^GCGCC
MfeI          794  CAATTG               C^AATTG
MslI         1638  CATACCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          794  CAATTG               C^AATTG
NarI          118  GGCGCC               GG^CGCC
NcoI         1440  CCATGG               C^CATGG
NheI          291  GCTAGC               G^CTAGC
NsiI          991  ATGCAT               ATGCA^T
PflFI        1232  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII          88  CAGCTG               CAG^CTG
SbfI         1576  CCTGCAGG             CCTGCA^GG
SfoI          118  GGCGCC               GGC^GCC
SmaI          235  CCCGGG               CCC^GGG
SmlI          541  CTCAAG               C^T[CT][GA]AG
Sse8387I     1576  CCTGCAGG             CCTGCA^GG
SspBI         831  TGTACA               T^GTACA
TaqI         1050  TCGA                 T^CGA
Tth111I      1232  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1050  TCGA                 T^CGA
XmaCI         235  CCCGGG               C^CCGGG
XmaI          235  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          832  GTAC                 GT^AC
              935  GTAC                
BbvCI         210  CCTCAGC              CCTCAGC(-5/-2)
               90  GCTGAGG             
BclI          522  TGATCA               T^GATCA
              945  TGATCA              
BfaI          292  CTAG                 C^TAG
              841  CTAG                
BglI          330  GCCGCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              356  GCCTGTCAGGC         
BsgI          112  GTGCAG               GTGCAG(16/14)
              453  GTGCAG              
BsmBI         185  CGTCTC               CGTCTC(1/5)
              890  CGTCTC              
BsrDI        1205  GCAATG               GCAATG(2/0)
             1591  GCAATG              
BsrFI         121  GCCGGC               [GA]^CCGG[CT]
              154  GCCGGC              
BstXI         442  CCATTAGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1440  CCATGGCTCTGG        
BstYI         799  GGATCC               [GA]^GATC[CT]
             1678  AGATCC              
Cfr10I        121  GCCGGC               [GA]^CCGG[CT]
              154  GCCGGC              
EarI         1409  CTCTTC               CTCTTC(1/4)
              265  GAAGAG              
FbaI          522  TGATCA               T^GATCA
              945  TGATCA              
HpaI           10  GTTAAC               GTT^AAC
             1015  GTTAAC              
Ksp632I      1409  CTCTTC               CTCTTC(1/4)
              265  GAAGAG              
MaeI          292  CTAG                 C^TAG
              841  CTAG                
MflI          799  GGATCC               [GA]^GATC[CT]
             1678  AGATCC              
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
              331  CCGCTG              
NaeI          121  GCCGGC               GCC^GGC
              154  GCCGGC              
NgoMIV        121  GCCGGC               G^CCGGC
              154  GCCGGC              
PleI         1276  GACTC                GAGTC(4/5)
             1300  GACTC               
RsaI          832  GTAC                 GT^AC
              935  GTAC                
SapI          265  GAAGAGC              GCTCTTC(1/4)
             1408  GCTCTTC             
TfiI          790  GATTC                G^A[AT]TC
              837  GATTC               
XhoII         799  GGATCC               [GA]^GATC[CT]
             1678  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AluI        
AlwI        ApaI        ApoI        AvaI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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