Restriction map for pF1KSDA1458
 (Linear) (Six-base) MAPSORT of: KIAA1458.seq  Check: 2919  from: 1  to: 1743



KIAA1458.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AccI GT'mk_AC

Cuts at:      0    1004    1743 
   Size:       1004     739
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     758    1337    1743 
   Size:        758     579     406

  Fragments arranged by size: 

                 758     579     406
 
AfeI AGC'GCT

Cuts at:      0    1560    1743 
   Size:       1560     183
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893    1660    1699    1743 
   Size:        893     767      39      44

  Fragments arranged by size: 

                 893     767      44      39
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57    1743 
   Size:         57    1686
 
ApaI G_GGCC'C

Cuts at:      0     137     236     247    1743 
   Size:        137      99      11    1496

  Fragments arranged by size: 

                1496     137      99      11
 
ApoI r'AATT_y

Cuts at:      0     552     696    1077    1305    1375    1743 
   Size:        552     144     381     228      70     368

  Fragments arranged by size: 

                 552     381     368     228     144      70
 
AvaI C'yCGr_G

Cuts at:      0     102     191     235     246    1103    1743 
   Size:        102      89      44      11     857     640

  Fragments arranged by size: 

                 857     640     102      89      44      11
 
BamHI G'GATC_C

Cuts at:      0     799    1743 
   Size:        799     944
 
BanI G'GyrC_C

Cuts at:      0     118    1743 
   Size:        118    1625
 
BanII G_rGCy'C

Cuts at:      0     137     236     247    1743 
   Size:        137      99      11    1496

  Fragments arranged by size: 

                1496     137      99      11
 
BbeI G_GCGC'C

Cuts at:      0     122    1743 
   Size:        122    1621
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1231    1743 
   Size:       1231     512
 
BbvCI CC'TCA_GC

Cuts at:      0      91     211    1743 
   Size:         91     120    1532

  Fragments arranged by size: 

                1532     120      91
 
BclI T'GATC_A

Cuts at:      0     522     945    1743 
   Size:        522     423     798

  Fragments arranged by size: 

                 798     522     423
 
BfrBI ATG'CAT

Cuts at:      0     993    1743 
   Size:        993     750
 
BglI GCCn_nnn'nGGC

Cuts at:      0     336     362    1743 
   Size:        336      26    1381

  Fragments arranged by size: 

                1381     336      26
 
Bme1580I G_kGCm'C

Cuts at:      0     137     236     247    1623    1743 
   Size:        137      99      11    1376     120

  Fragments arranged by size: 

                1376     137     120      99      11
 
BmgBI CAC'GTC

Cuts at:      0     890    1743 
   Size:        890     853
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     649    1610    1743 
   Size:        649     961     133

  Fragments arranged by size: 

                 961     649     133
 
BmtI G_CTAG'C

Cuts at:      0     295    1743 
   Size:        295    1448
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     370    1352    1743 
   Size:         91     279     982     391

  Fragments arranged by size: 

                 982     391     279      91
 
Bpu10I CC'TnA_GC

Cuts at:      0      91     211    1061    1743 
   Size:         91     120     850     682

  Fragments arranged by size: 

                 850     682     120      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526    1743 
   Size:        526    1217
 
BsaHI Gr'CG_yC

Cuts at:      0     119     184     278    1743 
   Size:        119      65      94    1465

  Fragments arranged by size: 

                1465     119      94      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     268     298     343     373    1743 
   Size:         28     240      30      45      30    1370

  Fragments arranged by size: 

                1370     240      45      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     108     162     179     385     388     391     394    1485 
   Size:        108      54      17     206       3       3       3    1091

Cuts at:   1485    1743 
   Size:        258

  Fragments arranged by size: 

                1091     258     206     108      54      17       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     365    1543    1630    1743 
   Size:        365    1178      87     113

  Fragments arranged by size: 

                1178     365     113      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     474    1743 
   Size:        133     341    1269

  Fragments arranged by size: 

                1269     341     133
 
BsiEI CG_ry'CG

Cuts at:      0    1054    1743 
   Size:       1054     689
 
BsiHKAI G_wGCw'C

Cuts at:      0    1525    1743 
   Size:       1525     218
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     191     896    1743 
   Size:        191     705     847

  Fragments arranged by size: 

                 847     705     191
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     236     247    1525    1623    1743 
   Size:        137      99      11    1278      98     120

  Fragments arranged by size: 

                1278     137     120      99      98      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1584    1743 
   Size:       1584     159
 
BsrBI CCG'CTC

Cuts at:      0     101    1743 
   Size:        101    1642
 
BsrDI GCAATG_nn'

Cuts at:      0    1212    1598    1743 
   Size:       1212     386     145

  Fragments arranged by size: 

                1212     386     145
 
BsrFI r'CCGG_y

Cuts at:      0     121     154    1743 
   Size:        121      33    1589

  Fragments arranged by size: 

                1589     121      33
 
BsrGI T'GTAC_A

Cuts at:      0     831    1743 
   Size:        831     912
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     449    1447    1743 
   Size:        449     998     296

  Fragments arranged by size: 

                 998     449     296
 
BstYI r'GATC_y

Cuts at:      0     799    1678    1743 
   Size:        799     879      65

  Fragments arranged by size: 

                 879     799      65
 
BstZ17I GTA'TAC

Cuts at:      0    1005    1743 
   Size:       1005     738
 
Bsu36I CC'TnA_GG

Cuts at:      0    1346    1743 
   Size:       1346     397
 
BtgI C'CryG_G

Cuts at:      0    1440    1743 
   Size:       1440     303
 
BtsI GCAGTG_nn'

Cuts at:      0     633    1743 
   Size:        633    1110
 
EarI CTCTTCn'nnn_

Cuts at:      0     260    1415    1743 
   Size:        260    1155     328

  Fragments arranged by size: 

                1155     328     260
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     267    1743 
   Size:        267    1476
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     370     758    1337    1352    1743 
   Size:         91     279     388     579      15     391

  Fragments arranged by size: 

                 579     391     388     279      91      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1367    1743 
   Size:       1367     376
 
HaeII r_GCGC'y

Cuts at:      0     122     218     357    1562    1743 
   Size:        122      96     139    1205     181

  Fragments arranged by size: 

                1205     181     139     122      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     268     300     343     375    1743 
   Size:         30     238      32      43      32    1368

  Fragments arranged by size: 

                1368     238      43      32      32      30
 
HincII GTy'rAC

Cuts at:      0      12    1017    1275    1743 
   Size:         12    1005     258     468

  Fragments arranged by size: 

                1005     468     258      12
 
HpaI GTT'AAC

Cuts at:      0      12    1017    1743 
   Size:         12    1005     726

  Fragments arranged by size: 

                1005     726      12
 
KasI G'GCGC_C

Cuts at:      0     118    1743 
   Size:        118    1625
 
MfeI C'AATT_G

Cuts at:      0     794    1743 
   Size:        794     949
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41    1705    1743 
   Size:         41    1664      38

  Fragments arranged by size: 

                1664      41      38
 
MslI CAynn'nnrTG

Cuts at:      0    1642    1743 
   Size:       1642     101
 
MspA1I CmG'CkG

Cuts at:      0      90     333    1743 
   Size:         90     243    1410

  Fragments arranged by size: 

                1410     243      90
 
NaeI GCC'GGC

Cuts at:      0     123     156    1743 
   Size:        123      33    1587

  Fragments arranged by size: 

                1587     123      33
 
NarI GG'CG_CC

Cuts at:      0     119    1743 
   Size:        119    1624
 
NcoI C'CATG_G

Cuts at:      0    1440    1743 
   Size:       1440     303
 
NgoMIV G'CCGG_C

Cuts at:      0     121     154    1743 
   Size:        121      33    1589

  Fragments arranged by size: 

                1589     121      33
 
NheI G'CTAG_C

Cuts at:      0     291    1743 
   Size:        291    1452
 
NsiI A_TGCA'T

Cuts at:      0     995    1743 
   Size:        995     748
 
PfoI T'CCnGG_A

Cuts at:      0     690    1743 
   Size:        690    1053
 
PsiI TTA'TAA

Cuts at:      0     809    1743 
   Size:        809     934
 
PspOMI G'GGCC_C

Cuts at:      0     133     232     243    1743 
   Size:        133      99      11    1500

  Fragments arranged by size: 

                1500     133      99      11
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1248    1280    1743 
   Size:       1248      32     463

  Fragments arranged by size: 

                1248     463      32
 
PstI C_TGCA'G

Cuts at:      0      53    1392    1581    1743 
   Size:         53    1339     189     162

  Fragments arranged by size: 

                1339     189     162      53
 
PvuII CAG'CTG

Cuts at:      0      90    1743 
   Size:         90    1653
 
SapI GCTCTTCn'nnn_

Cuts at:      0     260    1415    1743 
   Size:        260    1155     328

  Fragments arranged by size: 

                1155     328     260
 
SbfI CC_TGCA'GG

Cuts at:      0    1581    1743 
   Size:       1581     162
 
SfcI C'TryA_G

Cuts at:      0      49     668     752    1388    1577    1743 
   Size:         49     619      84     636     189     166

  Fragments arranged by size: 

                 636     619     189     166      84      49
 
SfoI GGC'GCC

Cuts at:      0     120    1743 
   Size:        120    1623
 
SmaI CCC'GGG

Cuts at:      0     237    1743 
   Size:        237    1506
 
SmlI C'TyrA_G

Cuts at:      0     541    1743 
   Size:        541    1202
 
StyI C'CwwG_G

Cuts at:      0     129     203    1440    1743 
   Size:        129      74    1237     303

  Fragments arranged by size: 

                1237     303     129      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1086    1743 
   Size:       1086     657
 
TatI w'GTAC_w

Cuts at:      0     831    1743 
   Size:        831     912
 
Tth111I GACn'n_nGTC

Cuts at:      0    1235    1743 
   Size:       1235     508
 
XmaI C'CCGG_G

Cuts at:      0     235    1743 
   Size:        235    1508
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AlfI    AlwNI     ApaI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BclI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     EarI     EciI  Eco57MI    EcoNI
    HaeII    Hin4I   HincII     HpaI     KasI     MfeI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI     PfoI
     PsiI   PspOMI     PsrI     PstI    PvuII     SapI     SbfI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BcgI    BciVI    BglII     BlpI     BplI     BsaI    BsaAI    BsaBI
    BsaWI    BsiWI     BsmI    BspEI    BspHI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     KpnI     MluI     MscI     NdeI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XhoI     XmnI     ZraI
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