Restriction map for pF1KSDA1445
##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AflIII      AhdI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BamHI       BciVI       BclI        BfrI        BglII       
BlnI        BsaBI       BseAI       BsiWI       BsmI        
BspDI       BspEI       BspLU11I    Bst1107I    BstBI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        Eam1105I    Eco47III    Eco81I      EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MflI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PmeI        PshBI       PvuI        RsrII       
SalI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SspI        SwaI        XbaI        
XhoII       


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AclI         1739  AACGTT               AA^CGTT
AcsI         3289  GAATTC               [GA]^AATT[CT]
AgeI         1291  ACCGGT               A^CCGGT
ApoI         3289  GAATTC               [GA]^AATT[CT]
Asp700I       582  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1636  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3113  TGGCCA               TGG^CCA
BbeI         1568  GGCGCC               GGCGC^C
BbrPI        1552  CACGTG               CAC^GTG
BbvCI         645  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1745  GCAGCACACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2765  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2765  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1291  ACCGGT               [AT]^CCGG[AT]
BsiEI         576  CGGCCG               CG[GA][CT]^CG
Bsp1407I     3365  TGTACA               T^GTACA
BspHI        1016  TCATGA               T^CATGA
BsrDI        3347  CATTGC               GCAATG(2/0)
BsrGI        3365  TGTACA               T^GTACA
BssSI        1420  CTCGTG               CACGAG(-5/-1)
BstEII       1844  GGTCACC              G^GT[ACGT]ACC
BstPI        1844  GGTCACC              G^GT[ACGT]ACC
BstXI        2893  CCAGAAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2765  GCTCAGC              GC^T[ACGT]AGC
EagI          576  CGGCCG               C^GGCCG
EclXI         576  CGGCCG               C^GGCCG
Eco52I        576  CGGCCG               C^GGCCG
EcoO65I      1844  GGTCACC              G^GT[ACGT]ACC
EcoRI        3289  GAATTC               G^AATTC
KasI         1568  GGCGCC               G^GCGCC
KspI         2474  CCGCGG               CCGC^GG
MluNI        3113  TGGCCA               TGG^CCA
MscI         3113  TGGCCA               TGG^CCA
MseI          845  TTAA                 T^TAA
NarI         1568  GGCGCC               GG^CGCC
PaeR7I       1753  CTCGAG               C^TCGAG
PflFI        1636  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1291  ACCGGT               A^CCGGT
PmaCI        1552  CACGTG               CAC^GTG
PmlI         1552  CACGTG               CAC^GTG
PshAI        2839  GACATCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1739  AACGTT               AA^CGTT
RcaI         1016  TCATGA               T^CATGA
SacII        2474  CCGCGG               CCGC^GG
SapI         1617  GCTCTTC              GCTCTTC(1/4)
SbfI         1323  CCTGCAGG             CCTGCA^GG
SexAI        1430  ACCTGGT              A^CC[AT]GGT
SfoI         1568  GGCGCC               GGC^GCC
SmlI         1753  CTCGAG               C^T[CT][GA]AG
Sse8387I     1323  CCTGCAGG             CCTGCA^GG
SspBI        3365  TGTACA               T^GTACA
Tru9I         845  TTAA                 T^TAA
Tth111I      1636  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1753  CTCGAG               C^TCGAG
XmnI          582  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AlwI         1910  GATCC                GGATC(4/5)
             3318  GATCC               
ApaI         1700  GGGCCC               GGGCC^C
             2577  GGGCCC              
BfaI          152  CTAG                 C^TAG
             3157  CTAG                
BsaAI         553  TACGTG               [CT]AC^GT[GA]
             1552  CACGTG              
BspMI         329  ACCTGC               ACCTGC(4/8)
             2401  ACCTGC              
BsrBI         507  CCGCTC               CCGCTC(-3/-3)
             2556  GAGCGG              
BssHII       1613  GCGCGC               G^CGCGC
             2408  GCGCGC              
DrdI         2001  GACCCCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2748  GACCTCATGGTC        
EaeI          576  CGGCCG               [CT]^GGCC[GA]
             3113  TGGCCA              
MaeI          152  CTAG                 C^TAG
             3157  CTAG                
NaeI          574  GCCGGC               GCC^GGC
             2957  GCCGGC              
NgoMIV        574  GCCGGC               G^CCGGC
             2957  GCCGGC              
NspI         1690  GCATGC               [GA]CATG^[CT]
             2222  GCATGC              
SphI         1690  GCATGC               GCATG^C
             2222  GCATGC              
StuI         2885  AGGCCT               AGG^CCT
             3419  AGGCCT              
Tsp509I      1264  AATT                 ^AATT
             3290  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwNI       ApaLI       AspHI       AvaI        
AvaII       AviII       BanI        BanII       BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com