Restriction map for pF1KSDA1445
 (Linear) (Six-base) MAPSORT of: KIAA1445.seq  Check: 5330  from: 1  to: 3453



KIAA1445.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2410    3453 
   Size:       2410    1043
 
AclI AA'CG_TT

Cuts at:      0    1740    3453 
   Size:       1740    1713
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     454    1516    3453 
   Size:        423      31    1062    1937

  Fragments arranged by size: 

                1937    1062     423      31
 
AgeI A'CCGG_T

Cuts at:      0    1291    3453 
   Size:       1291    2162
 
AleI CACnn'nnGTG

Cuts at:      0     926    3453 
   Size:        926    2527
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2116    2148    2242    2274    2374    2406    3453 
   Size:       2116      32      94      32     100      32    1047

  Fragments arranged by size: 

                2116    1047     100      94      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288    2210    2993    3453 
   Size:        288    1922     783     460

  Fragments arranged by size: 

                1922     783     460     288
 
ApaI G_GGCC'C

Cuts at:      0    1704    2581    3453 
   Size:       1704     877     872

  Fragments arranged by size: 

                1704     877     872
 
ApaLI G'TGCA_C

Cuts at:      0     621    2637    2928    3453 
   Size:        621    2016     291     525

  Fragments arranged by size: 

                2016     621     525     291
 
ApoI r'AATT_y

Cuts at:      0    3289    3453 
   Size:       3289     164
 
AvaI C'yCGr_G

Cuts at:      0     368    1044    1378    1564    1703    1753    2299    2597 
   Size:        368     676     334     186     139      50     546     298

Cuts at:   2597    2848    3415    3453 
   Size:        251     567      38

  Fragments arranged by size: 

                 676     567     546     368     334     298     251     186
                 139      50      38
 
BanI G'GyrC_C

Cuts at:      0    1276    1474    1486    1568    2160    3256    3453 
   Size:       1276     198      12      82     592    1096     197

  Fragments arranged by size: 

                1276    1096     592     198     197      82      12
 
BanII G_rGCy'C

Cuts at:      0     272     369     740    1385    1580    1704    1908    2188 
   Size:        272      97     371     645     195     124     204     280

Cuts at:   2188    2581    2654    2946    2975    3142    3149    3453 
   Size:        393      73     292      29     167       7     304

  Fragments arranged by size: 

                 645     393     371     304     292     280     272     204
                 195     167     124      97      73      29       7
 
BbeI G_GCGC'C

Cuts at:      0    1572    3453 
   Size:       1572    1881
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     118     332     535    3453 
   Size:        118     214     203    2918

  Fragments arranged by size: 

                2918     214     203     118
 
BbvCI CC'TCA_GC

Cuts at:      0     646    3453 
   Size:        646    2807
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1734    1768    3453 
   Size:       1734      34    1685

  Fragments arranged by size: 

                1734    1685      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1529    1566    2096    2884    2897    3453 
   Size:       1529      37     530     788      13     556

  Fragments arranged by size: 

                1529     788     556     530      37      13
 
BlpI GC'TnA_GC

Cuts at:      0    2766    3453 
   Size:       2766     687
 
Bme1580I G_kGCm'C

Cuts at:      0     625    1489    1704    2165    2376    2581    2641    2932 
   Size:        625     864     215     461     211     205      60     291

Cuts at:   2932    3453 
   Size:        521

  Fragments arranged by size: 

                 864     625     521     461     291     215     211     205
                  60
 
BmgBI CAC'GTC

Cuts at:      0     975    2033    2291    3453 
   Size:        975    1058     258    1162

  Fragments arranged by size: 

                1162    1058     975     258
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2716    3453 
   Size:       2716     737
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     504    1779    3453 
   Size:        504    1275    1674

  Fragments arranged by size: 

                1674    1275     504
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     246     383     474    1015    1561    1678    1968 
   Size:        228      18     137      91     541     546     117     290

Cuts at:   1968    2211    3045    3453 
   Size:        243     834     408

  Fragments arranged by size: 

                 834     546     541     408     290     243     228     137
                 117      91      18
 
Bpu10I CC'TnA_GC

Cuts at:      0      76     298     646    3453 
   Size:         76     222     348    2807

  Fragments arranged by size: 

                2807     348     222      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     348    1282    2762    3453 
   Size:        348     934    1480     691

  Fragments arranged by size: 

                1480     934     691     348
 
BsaAI yAC'GTr

Cuts at:      0     555    1554    3453 
   Size:        555     999    1899

  Fragments arranged by size: 

                1899     999     555
 
BsaHI Gr'CG_yC

Cuts at:      0    1569    1640    2235    2338    2504    3453 
   Size:       1569      71     595     103     166     949

  Fragments arranged by size: 

                1569     949     595     166     103      71
 
BsaWI w'CCGG_w

Cuts at:      0    1291    3453 
   Size:       1291    2162
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2116    2146    2242    2272    2374    2404    3453 
   Size:       2116      30      96      30     102      30    1049

  Fragments arranged by size: 

                2116    1049     102      96      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     550     553     714    1558    2878    2965    2983    3085 
   Size:        550       3     161     844    1320      87      18     102

Cuts at:   3085    3453 
   Size:        368

  Fragments arranged by size: 

                1320     844     550     368     161     102      87      18
                   3
 
BseYI C'CCAG_C

Cuts at:      0      63     214     271     380    1721    2564    2610    3453 
   Size:         63     151      57     109    1341     843      46     843

  Fragments arranged by size: 

                1341     843     843     151     109      63      57      46
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1384    1456    1534    1585    2046    2220    2793    2839 
   Size:       1384      72      78      51     461     174     573      46

Cuts at:   2839    3107    3453 
   Size:        268     346

  Fragments arranged by size: 

                1384     573     461     346     268     174      78      72
                  51      46
 
BsiEI CG_ry'CG

Cuts at:      0     579    3453 
   Size:        579    2874
 
BsiHKAI G_wGCw'C

Cuts at:      0     625     740     923    1868    1908    2539    2641    2932 
   Size:        625     115     183     945      40     631     102     291

Cuts at:   2932    2975    3453 
   Size:         43     478

  Fragments arranged by size: 

                 945     631     625     478     291     183     115     102
                  43      40
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      26    1628    2452    3453 
   Size:         26    1602     824    1001

  Fragments arranged by size: 

                1602    1001     824      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     272     369     625     740     923    1385    1489    1580 
   Size:        272      97     256     115     183     462     104      91

Cuts at:   1580    1704    1868    1908    2165    2188    2376    2539    2581 
   Size:        124     164      40     257      23     188     163      42

Cuts at:   2581    2641    2654    2932    2946    2975    3142    3149    3453 
   Size:         60      13     278      14      29     167       7     304

  Fragments arranged by size: 

                 462     304     278     272     257     256     188     183
                 167     164     163     124     115     104      97      91
                  60      42      40      29      23      14      13       7
            
 
BspHI T'CATG_A

Cuts at:      0    1016    3453 
   Size:       1016    2437
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     338    2410    3453 
   Size:        338    2072    1043

  Fragments arranged by size: 

                2072    1043     338
 
BsrBI CCG'CTC

Cuts at:      0     509    2558    3453 
   Size:        509    2049     895

  Fragments arranged by size: 

                2049     895     509
 
BsrDI GCAATG_nn'

Cuts at:      0    3346    3453 
   Size:       3346     107
 
BsrFI r'CCGG_y

Cuts at:      0     574    1291    1368    2957    3079    3453 
   Size:        574     717      77    1589     122     374

  Fragments arranged by size: 

                1589     717     574     374     122      77
 
BsrGI T'GTAC_A

Cuts at:      0    3365    3453 
   Size:       3365      88
 
BssHII G'CGCG_C

Cuts at:      0    1613    2408    3453 
   Size:       1613     795    1045

  Fragments arranged by size: 

                1613    1045     795
 
BssSI C'ACGA_G

Cuts at:      0    1420    3453 
   Size:       1420    2033
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1557    2261    2433    2875    3128    3453 
   Size:       1557     704     172     442     253     325

  Fragments arranged by size: 

                1557     704     442     325     253     172
 
BstEII G'GTnAC_C

Cuts at:      0    1844    3453 
   Size:       1844    1609
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2900    3453 
   Size:       2900     553
 
BtgI C'CryG_G

Cuts at:      0     314     337     749    1508    1527    1840    1849    2005 
   Size:        314      23     412     759      19     313       9     156

Cuts at:   2005    2091    2341    2474    2581    3116    3284    3453 
   Size:         86     250     133     107     535     168     169

  Fragments arranged by size: 

                 759     535     412     314     313     250     169     168
                 156     133     107      86      23      19       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1128    1956    2696    3453 
   Size:       1128     828     740     757

  Fragments arranged by size: 

                1128     828     757     740
 
BtsI GCAGTG_nn'

Cuts at:      0     223     282     807     818     920    3167    3180    3453 
   Size:        223      59     525      11     102    2247      13     273

  Fragments arranged by size: 

                2247     525     273     223     102      59      13      11
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     842     877    3453 
   Size:        842      35    2576

  Fragments arranged by size: 

                2576     842      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     482    1422    2553    2875    3111    3453 
   Size:        482     940    1131     322     236     342

  Fragments arranged by size: 

                1131     940     482     342     322     236
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2007    2754    3453 
   Size:       2007     747     699

  Fragments arranged by size: 

                2007     747     699
 
EaeI y'GGCC_r

Cuts at:      0     576    3113    3453 
   Size:        576    2537     340

  Fragments arranged by size: 

                2537     576     340
 
EagI C'GGCC_G

Cuts at:      0     576    3453 
   Size:        576    2877
 
EarI CTCTTCn'nnn_

Cuts at:      0     442     470    1348    1624    3453 
   Size:        442      28     878     276    1829

  Fragments arranged by size: 

                1829     878     442     276      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2047    3266    3453 
   Size:       2047    1219     187

  Fragments arranged by size: 

                2047    1219     187
 
EcoICRI GAG'CTC

Cuts at:      0     738    1906    2973    3453 
   Size:        738    1168    1067     480

  Fragments arranged by size: 

                1168    1067     738     480
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228     246     383     423     454     474    1015    1516 
   Size:        228      18     137      40      31      20     541     501

Cuts at:   1516    1561    1678    1968    2211    3045    3453 
   Size:         45     117     290     243     834     408

  Fragments arranged by size: 

                 834     541     501     408     290     243     228     137
                 117      45      40      31      20      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     994    1435    2512    3453 
   Size:        994     441    1077     941

  Fragments arranged by size: 

                1077     994     941     441
 
EcoO109I rG'GnC_Cy

Cuts at:      0     166     187     774    1053    1104    1650    1700    1707 
   Size:        166      21     587     279      51     546      50       7

Cuts at:   1707    1941    2465    2517    2577    2664    3453 
   Size:        234     524      52      60      87     789

  Fragments arranged by size: 

                 789     587     546     524     279     234     166      87
                  60      52      51      50      21       7
 
EcoRI G'AATT_C

Cuts at:      0    3289    3453 
   Size:       3289     164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3330    3453 
   Size:       3330     123
 
FspI TGC'GCA

Cuts at:      0    1585    1594    2527    3453 
   Size:       1585       9     933     926

  Fragments arranged by size: 

                1585     933     926       9
 
HaeII r_GCGC'y

Cuts at:      0    1341    1504    1572    1610    2023    2993    3453 
   Size:       1341     163      68      38     413     970     460

  Fragments arranged by size: 

                1341     970     460     413     163      68      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     551     583    1625    1657    2454    2486    3453 
   Size:        551      32    1042      32     797      32     967

  Fragments arranged by size: 

                1042     967     797     551      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1568    3453 
   Size:       1568    1885
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1790    3453 
   Size:       1790    1663
 
MscI TGG'CCA

Cuts at:      0    3115    3453 
   Size:       3115     338
 
MslI CAynn'nnrTG

Cuts at:      0     926    1015    1871    2107    3283    3453 
   Size:        926      89     856     236    1176     170

  Fragments arranged by size: 

                1176     926     856     236     170      89
 
MspA1I CmG'CkG

Cuts at:      0     288     384     683     715    1934    2388    2476    2531 
   Size:        288      96     299      32    1219     454      88      55

Cuts at:   2531    3453 
   Size:        922

  Fragments arranged by size: 

                1219     922     454     299     288      96      88      55
                  32
 
NaeI GCC'GGC

Cuts at:      0     576    2959    3453 
   Size:        576    2383     494

  Fragments arranged by size: 

                2383     576     494
 
NarI GG'CG_CC

Cuts at:      0    1569    3453 
   Size:       1569    1884
 
NcoI C'CATG_G

Cuts at:      0    1840    1849    3284    3453 
   Size:       1840       9    1435     169

  Fragments arranged by size: 

                1840    1435     169       9
 
NgoMIV G'CCGG_C

Cuts at:      0     574    2957    3453 
   Size:        574    2383     496

  Fragments arranged by size: 

                2383     574     496
 
NspI r_CATG'y

Cuts at:      0    1694    2226    3453 
   Size:       1694     532    1227

  Fragments arranged by size: 

                1694    1227     532
 
PasI CC'CwG_GG

Cuts at:      0      46     729    1226    2980    3453 
   Size:         46     683     497    1754     473

  Fragments arranged by size: 

                1754     683     497     473      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     482    2171    3111    3453 
   Size:        482    1689     940     342

  Fragments arranged by size: 

                1689     940     482     342
 
PfoI T'CCnGG_A

Cuts at:      0     144     400    1984    2188    3453 
   Size:        144     256    1584     204    1265

  Fragments arranged by size: 

                1584    1265     256     204     144
 
PmlI CAC'GTG

Cuts at:      0    1554    3453 
   Size:       1554    1899
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2116    2148    2242    2274    2374    2406    3453 
   Size:       2116      32      94      32     100      32    1047

  Fragments arranged by size: 

                2116    1047     100      94      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     774    1053    1104    1650    1707    2465    2517    3453 
   Size:        774     279      51     546      57     758      52     936

  Fragments arranged by size: 

                 936     774     758     546     279      57      52      51
            
 
PshAI GACnn'nnGTC

Cuts at:      0    2843    3453 
   Size:       2843     610
 
PspOMI G'GGCC_C

Cuts at:      0    1700    2577    3453 
   Size:       1700     877     876

  Fragments arranged by size: 

                1700     877     876
 
PspXI vC'TCGA_Gb

Cuts at:      0    1753    3453 
   Size:       1753    1700
 
PstI C_TGCA'G

Cuts at:      0     162     335    1079    1328    2438    3453 
   Size:        162     173     744     249    1110    1015

  Fragments arranged by size: 

                1110    1015     744     249     173     162
 
PvuII CAG'CTG

Cuts at:      0     288     384     715    3453 
   Size:        288      96     331    2738

  Fragments arranged by size: 

                2738     331     288      96
 
SacI G_AGCT'C

Cuts at:      0     740    1908    2975    3453 
   Size:        740    1168    1067     478

  Fragments arranged by size: 

                1168    1067     740     478
 
SacII CC_GC'GG

Cuts at:      0    2477    3453 
   Size:       2477     976
 
SanDI GG'GwC_CC

Cuts at:      0     774    1650    1707    3453 
   Size:        774     876      57    1746

  Fragments arranged by size: 

                1746     876     774      57
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1624    3453 
   Size:       1624    1829
 
SbfI CC_TGCA'GG

Cuts at:      0    1328    3453 
   Size:       1328    2125
 
SexAI A'CCwGG_T

Cuts at:      0    1430    3453 
   Size:       1430    2023
 
SfcI C'TryA_G

Cuts at:      0     158     331    1075    1324    2434    3030    3453 
   Size:        158     173     744     249    1110     596     423

  Fragments arranged by size: 

                1110     744     596     423     249     173     158
 
SfoI GGC'GCC

Cuts at:      0    1570    3453 
   Size:       1570    1883
 
SmaI CCC'GGG

Cuts at:      0     370    1046    1566    1705    2599    3453 
   Size:        370     676     520     139     894     854

  Fragments arranged by size: 

                 894     854     676     520     370     139
 
SmlI C'TyrA_G

Cuts at:      0    1753    3453 
   Size:       1753    1700
 
SphI G_CATG'C

Cuts at:      0    1694    2226    3453 
   Size:       1694     532    1227

  Fragments arranged by size: 

                1694    1227     532
 
SrfI GCCC'GGGC

Cuts at:      0    1046    3453 
   Size:       1046    2407
 
StuI AGG'CCT

Cuts at:      0    2887    3421    3453 
   Size:       2887     534      32

  Fragments arranged by size: 

                2887     534      32
 
StyI C'CwwG_G

Cuts at:      0    1840    1849    3284    3453 
   Size:       1840       9    1435     169

  Fragments arranged by size: 

                1840    1435     169       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1408    2470    3297    3453 
   Size:       1408    1062     827     156

  Fragments arranged by size: 

                1408    1062     827     156
 
TatI w'GTAC_w

Cuts at:      0     941    1461    3275    3365    3453 
   Size:        941     520    1814      90      88

  Fragments arranged by size: 

                1814     941     520      90      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     923     955    1785    1817    3453 
   Size:        923      32     830      32    1636

  Fragments arranged by size: 

                1636     923     830      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1639    3453 
   Size:       1639    1814
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      43     633    1195    1847    3453 
   Size:         43     590     562     652    1606

  Fragments arranged by size: 

                1606     652     590     562      43
 
XhoI C'TCGA_G

Cuts at:      0    1753    3453 
   Size:       1753    1700
 
XmaI C'CCGG_G

Cuts at:      0     368    1044    1564    1703    2597    3453 
   Size:        368     676     520     139     894     856

  Fragments arranged by size: 

                 894     856     676     520     368     139
 
XmnI GAAnn'nnTTC

Cuts at:      0     586    3453 
   Size:        586    2867
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AgeI     AleI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII
     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfcI     SfoI
     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AhdI     AlfI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI
    BfrBI    BglII     BmtI    BpuEI    BsaBI    BsiWI     BsmI    BspEI
    BstBI    BstYI  BstZ17I   Bsu36I     ClaI     DraI    EcoRV     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PsiI
     PsrI     PvuI    RsrII     SalI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SspI     SwaI     XbaI     ZraI
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