(Circular) (Six-base) MAPSORT of: pF1KA1435.seq Check: 2617 from: 1 to: 4343 pF1KA1435 4343 bp With 182 enzymes: SgfI * July 31, 2008 09:34 .. AccI GT'mk_AC Cuts at: 1381 1381 Size: 4343 Acc65I G'GTAC_C Cuts at: 1359 1359 Size: 4343 AclI AA'CG_TT Cuts at: 4183 4183 Size: 4343 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 422 456 673 805 844 2168 2600 3347 3615 Size: 34 217 132 39 1324 432 747 268 Cuts at: 3615 422 Size: 1150 Fragments arranged by size: 1324 1150 747 432 268 217 132 39 34 AfeI AGC'GCT Cuts at: 1093 1093 Size: 4343 AflIII A'CryG_T Cuts at: 668 670 1220 1695 2799 3776 668 Size: 2 550 475 1104 977 1235 Fragments arranged by size: 1235 1104 977 550 475 2 AgeI A'CCGG_T Cuts at: 2731 2731 Size: 4343 AlwNI CAG_nnn'CTG Cuts at: 849 1648 3215 849 Size: 799 1567 1977 Fragments arranged by size: 1977 1567 799 ApaLI G'TGCA_C Cuts at: 531 3113 531 Size: 2582 1761 ApoI r'AATT_y Cuts at: 388 1347 3696 3952 388 Size: 959 2349 256 779 Fragments arranged by size: 2349 959 779 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4343 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4343 AvaI C'yCGr_G Cuts at: 553 1363 553 Size: 810 3533 BamHI G'GATC_C Cuts at: 1368 1368 Size: 4343 BanI G'GyrC_C Cuts at: 173 1359 1773 2021 2056 173 Size: 1186 414 248 35 2460 Fragments arranged by size: 2460 1186 414 248 35 BanII G_rGCy'C Cuts at: 1357 2387 1357 Size: 1030 3313 BbeI G_GCGC'C Cuts at: 1777 2025 1777 Size: 248 4095 BciVI GTATCCnnnnn_n' Cuts at: 2241 3002 4343 2241 Size: 761 1341 2241 Fragments arranged by size: 2241 1341 761 BclI T'GATC_A Cuts at: 224 494 1863 224 Size: 270 1369 2704 Fragments arranged by size: 2704 1369 270 BglI GCCn_nnn'nGGC Cuts at: 112 190 112 Size: 78 4265 BglII A'GATC_T Cuts at: 1280 1858 3895 1280 Size: 578 2037 1728 Fragments arranged by size: 2037 1728 578 BlpI GC'TnA_GC Cuts at: 157 1455 157 Size: 1298 3045 Bme1580I G_kGCm'C Cuts at: 176 535 1968 2061 3117 176 Size: 359 1433 93 1056 1402 Fragments arranged by size: 1433 1402 1056 359 93 BmgBI CAC'GTC Cuts at: 572 572 Size: 4343 BmrI ACTGGGnnnn_n' Cuts at: 1099 1331 1555 1636 1971 4115 1099 Size: 232 224 81 335 2144 1327 Fragments arranged by size: 2144 1327 335 232 224 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 4343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 111 111 Size: 4343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1080 1519 2398 2890 3188 1080 Size: 439 879 492 298 2235 Fragments arranged by size: 2235 879 492 439 298 BsaI GGTCTCn'nnnn_ Cuts at: 4047 4047 Size: 4343 BsaAI yAC'GTr Cuts at: 671 1696 671 Size: 1025 3318 BsaBI GATnn'nnATC Cuts at: 6 1169 1857 3894 6 Size: 1163 688 2037 455 Fragments arranged by size: 2037 1163 688 455 BsaHI Gr'CG_yC Cuts at: 182 1774 2022 2724 4220 182 Size: 1592 248 702 1496 305 Fragments arranged by size: 1592 1496 702 305 248 BsaWI w'CCGG_w Cuts at: 1532 2053 2731 3005 3152 3563 1532 Size: 521 678 274 147 411 2312 Fragments arranged by size: 2312 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 932 932 Size: 4343 BseYI C'CCAG_C Cuts at: 289 438 1026 1768 2526 3103 3599 289 Size: 149 588 742 758 577 496 1033 Fragments arranged by size: 1033 758 742 588 577 496 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 862 1101 862 Size: 239 4104 BsiEI CG_ry'CG Cuts at: 104 116 194 1269 1885 1931 3139 104 Size: 12 78 1075 616 46 1208 1308 Fragments arranged by size: 1308 1208 1075 616 78 46 12 BsiHKAI G_wGCw'C Cuts at: 535 1357 2135 2325 3117 535 Size: 822 778 190 792 1761 Fragments arranged by size: 1761 822 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 885 885 Size: 4343 Bsp1286I G_dGCh'C Cuts at: 176 535 1357 1968 2061 2135 2325 2387 3117 Size: 359 822 611 93 74 190 62 730 Cuts at: 3117 176 Size: 1402 Fragments arranged by size: 1402 822 730 611 359 190 93 74 62 BspEI T'CCGG_A Cuts at: 3563 3563 Size: 4343 BspHI T'CATG_A Cuts at: 339 831 1194 3519 339 Size: 492 363 2325 1163 Fragments arranged by size: 2325 1163 492 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1393 1607 1909 2290 1393 Size: 214 302 381 3446 Fragments arranged by size: 3446 381 302 214 BsrBI CCG'CTC Cuts at: 50 542 2635 2689 4173 4338 50 Size: 492 2093 54 1484 165 55 Fragments arranged by size: 2093 1484 492 165 55 54 BsrDI GCAATG_nn' Cuts at: 1232 2255 1232 Size: 1023 3320 BsrFI r'CCGG_y Cuts at: 502 2341 2522 2731 502 Size: 1839 181 209 2114 Fragments arranged by size: 2114 1839 209 181 BssSI C'ACGA_G Cuts at: 2614 2972 2614 Size: 358 3985 BstAPI GCAn_nnn'nTGC Cuts at: 1055 1720 1055 Size: 665 3678 BstBI TT'CG_AA Cuts at: 2704 2704 Size: 4343 BstEII G'GTnAC_C Cuts at: 1251 1251 Size: 4343 BstXI CCAn_nnnn'nTGG Cuts at: 373 992 1026 373 Size: 619 34 3690 Fragments arranged by size: 3690 619 34 BstYI r'GATC_y Cuts at: 1 1280 1368 1835 1858 2193 2439 3451 3537 Size: 1279 88 467 23 335 246 1012 86 Cuts at: 3537 3549 3895 1 Size: 12 346 449 Fragments arranged by size: 1279 1012 467 449 346 335 246 88 86 23 12 BtgI C'CryG_G Cuts at: 107 1226 107 Size: 1119 3224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2293 2474 2637 2293 Size: 181 163 3999 Fragments arranged by size: 3999 181 163 BtsI GCAGTG_nn' Cuts at: 914 1044 1586 914 Size: 130 542 3671 Fragments arranged by size: 3671 542 130 DraI TTT'AAA Cuts at: 1342 1342 Size: 4343 DraIII CAC_nnn'GTG Cuts at: 488 488 Size: 4343 DrdI GACnn_nn'nnGTC Cuts at: 2049 2907 2049 Size: 858 3485 EaeI y'GGCC_r Cuts at: 113 191 230 479 504 1928 2102 2493 2520 Size: 78 39 249 25 1424 174 391 27 Cuts at: 2520 3865 113 Size: 1345 591 Fragments arranged by size: 1424 1345 591 391 249 174 78 39 27 25 EagI C'GGCC_G Cuts at: 113 191 1928 113 Size: 78 1737 2528 Fragments arranged by size: 2528 1737 78 EarI CTCTTCn'nnn_ Cuts at: 754 929 2366 2576 754 Size: 175 1437 210 2521 Fragments arranged by size: 2521 1437 210 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 914 2551 2861 3007 4153 914 Size: 1637 310 146 1146 1104 Fragments arranged by size: 1637 1146 1104 310 146 EcoICRI GAG'CTC Cuts at: 1355 1355 Size: 4343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 111 422 456 673 805 844 2168 2600 3347 Size: 311 34 217 132 39 1324 432 747 Cuts at: 3347 3615 111 Size: 268 839 Fragments arranged by size: 1324 839 747 432 311 268 217 132 39 34 EcoO109I rG'GnC_Cy Cuts at: 729 1482 729 Size: 753 3590 EcoRI G'AATT_C Cuts at: 1347 1347 Size: 4343 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 980 2362 980 Size: 1382 2961 FspI TGC'GCA Cuts at: 2124 2124 Size: 4343 HaeII r_GCGC'y Cuts at: 1095 1777 2025 3047 4026 1095 Size: 682 248 1022 979 1412 Fragments arranged by size: 1412 1022 979 682 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2185 2215 2217 2247 2185 Size: 30 2 30 4281 Fragments arranged by size: 4281 30 30 2 HincII GTy'rAC Cuts at: 1382 1382 Size: 4343 HindIII A'AGCT_T Cuts at: 655 655 Size: 4343 KasI G'GCGC_C Cuts at: 1773 2021 1773 Size: 248 4095 KpnI G_GTAC'C Cuts at: 1363 1363 Size: 4343 MluI A'CGCG_T Cuts at: 3776 3776 Size: 4343 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 932 3014 3198 932 Size: 2082 184 2077 Fragments arranged by size: 2082 2077 184 MscI TGG'CCA Cuts at: 481 2104 481 Size: 1623 2720 MslI CAynn'nnrTG Cuts at: 778 1225 2332 2459 778 Size: 447 1107 127 2662 Fragments arranged by size: 2662 1107 447 127 MspA1I CmG'CkG Cuts at: 741 1454 1768 2128 3141 3386 741 Size: 713 314 360 1013 245 1698 Fragments arranged by size: 1698 1013 713 360 314 245 NaeI GCC'GGC Cuts at: 2524 2524 Size: 4343 NarI GG'CG_CC Cuts at: 1774 2022 1774 Size: 248 4095 NcoI C'CATG_G Cuts at: 107 1226 107 Size: 1119 3224 NgoMIV G'CCGG_C Cuts at: 2522 2522 Size: 4343 NspI r_CATG'y Cuts at: 552 1224 1396 1896 2803 552 Size: 672 172 500 907 2092 Fragments arranged by size: 2092 907 672 500 172 PciI A'CATG_T Cuts at: 1220 2799 1220 Size: 1579 2764 PflMI CCAn_nnn'nTGG Cuts at: 306 488 306 Size: 182 4161 PmeI GTTT'AAAC Cuts at: 1342 1342 Size: 4343 PmlI CAC'GTG Cuts at: 671 671 Size: 4343 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 3482 Size: 32 4311 PpuMI rG'GwC_Cy Cuts at: 729 729 Size: 4343 PshAI GACnn'nnGTC Cuts at: 275 275 Size: 4343 PstI C_TGCA'G Cuts at: 1051 1312 1390 2075 1051 Size: 261 78 685 3319 Fragments arranged by size: 3319 685 261 78 PvuI CG_AT'CG Cuts at: 104 104 Size: 4343 PvuII CAG'CTG Cuts at: 1768 2128 1768 Size: 360 3983 RsrII CG'GwC_CG Cuts at: 2538 2538 Size: 4343 SacI G_AGCT'C Cuts at: 1357 1357 Size: 4343 SalI G'TCGA_C Cuts at: 1380 1380 Size: 4343 SanDI GG'GwC_CC Cuts at: 729 729 Size: 4343 SapI GCTCTTCn'nnn_ Cuts at: 2366 2576 2366 Size: 210 4133 SbfI CC_TGCA'GG Cuts at: 1390 1390 Size: 4343 ScaI AGT'ACT Cuts at: 3860 3860 Size: 4343 SfcI C'TryA_G Cuts at: 33 1047 1308 1386 2071 3064 3255 33 Size: 1014 261 78 685 993 191 1121 Fragments arranged by size: 1121 1014 993 685 261 191 78 SfoI GGC'GCC Cuts at: 1775 2023 1775 Size: 248 4095 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4343 SmaI CCC'GGG Cuts at: 1365 1365 Size: 4343 SmlI C'TyrA_G Cuts at: 1095 1498 2413 2905 3167 1095 Size: 403 915 492 262 2271 Fragments arranged by size: 2271 915 492 403 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4343 SphI G_CATG'C Cuts at: 1396 1896 1396 Size: 500 3843 StyI C'CwwG_G Cuts at: 107 209 1226 1477 1831 107 Size: 102 1017 251 354 2619 Fragments arranged by size: 2619 1017 354 251 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 480 1279 1926 2059 2518 2727 480 Size: 799 647 133 459 209 2096 Fragments arranged by size: 2096 799 647 459 209 133 TatI w'GTAC_w Cuts at: 3858 3858 Size: 4343 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 893 925 1235 1267 3860 3892 893 Size: 32 310 32 2593 32 1344 Fragments arranged by size: 2593 1344 310 32 32 32 Tth111I GACn'n_nGTC Cuts at: 184 2140 184 Size: 1956 2387 XbaI T'CTAG_A Cuts at: 1374 1374 Size: 4343 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1245 1838 1245 Size: 593 3750 XmaI C'CCGG_G Cuts at: 1363 1363 Size: 4343 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI