################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1808 GTCTAC GT^[AC][GT]AC AflIII 1767 ACATGT A^C[GA][CT]GT AlwNI 2036 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 2365 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 758 GTGCTC G[AT]GC[AT]^C AspI 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1278 GGATCC G^GATCC BanI 2449 GGTGCC G^G[CT][GA]CC BciVI 2553 GGATAC GTATCC(6/5) BglII 2131 AGATCT A^GATCT BmyI 758 GTGCTC G[AGT]GC[ACT]^C BpmI 1037 CTCCAG CTGGAG(16/14) BsgI 998 CTGCAC GTGCAG(16/14) BsiHKAI 758 GTGCTC G[AT]GC[AT]^C BsiYI 1419 CCTTGTAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1419 CCTTGTAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 758 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 1770 TGTACA T^GTACA BspDI 1531 ATCGAT AT^CGAT BspHI 1710 TCATGA T^CATGA BspLU11I 1767 ACATGT A^CATGT BspMI 504 GCAGGT ACCTGC(4/8) BsrGI 1770 TGTACA T^GTACA BstAPI 422 GCATTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 1531 ATCGAT AT^CGAT DrdI 1223 GACAGCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1968 GAAGAG CTCTTC(1/4) EcoNI 1419 CCTTGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1714 GAATTC G^AATTC EcoRV 1555 GATATC GAT^ATC EcoT14I 1820 CCAAGG C^C[AT][AT]GG HapII 1152 CCGG C^CGG HpaII 1152 CCGG C^CGG Ksp632I 1968 GAAGAG CTCTTC(1/4) MfeI 152 CAATTG C^AATTG MspI 1152 CCGG C^CGG MunI 152 CAATTG C^AATTG NdeI 2341 CATATG CA^TATG NspI 1767 ACATGT [GA]CATG^[CT] PflFI 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1710 TCATGA T^CATGA SfcI 75 CTATAG C^T[GA][CT]AG SspBI 1770 TGTACA T^GTACA SspI 649 AATATT AAT^ATT StuI 1416 AGGCCT AGG^CCT StyI 1820 CCAAGG C^C[AT][AT]GG Tth111I 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1990 CCATCTTCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2365 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaII 697 GGACC G^G[AT]CC 1824 GGACC BalI 99 TGGCCA TGG^CCA 1297 TGGCCA BseRI 2045 CTCCTC GAGGAG(10/8) 2418 CTCCTC BsmAI 2506 GAGAC GTCTC(1/5) 412 GTCTC BssKI 232 CCTGG ^CC[ACGT]GG 1039 CCAGG BstNI 232 CCTGG CC^[AT]GG 1039 CCAGG BstYI 1278 GGATCC [GA]^GATC[CT] 2131 AGATCT Cac8I 1696 GCAGGC GC[ACGT]^[ACGT]GC 2125 GCCAGC EaeI 99 TGGCCA [CT]^GGCC[GA] 1297 TGGCCA EcoRII 232 CCTGG ^CC[AT]GG 1039 CCAGG EcoT22I 420 ATGCAT ATGCA^T 2459 ATGCAT MaeII 1623 ACGT A^CGT 1946 ACGT MflI 1278 GGATCC [GA]^GATC[CT] 2131 AGATCT MluNI 99 TGGCCA TGG^CCA 1297 TGGCCA MscI 99 TGGCCA TGG^CCA 1297 TGGCCA MspA1I 513 CAGCTG C[AC]G^C[GT]G 1128 CAGCTG MvaI 232 CCTGG CC^[AT]GG 1039 CCAGG NsiI 420 ATGCAT ATGCA^T 2459 ATGCAT PvuII 513 CAGCTG CAG^CTG 1128 CAGCTG ScrFI 232 CCTGG CC^[ACGT]GG 1039 CCAGG XhoII 1278 GGATCC [GA]^GATC[CT] 2131 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BclI BfaI BpuAI BsaJI BsmFI BsmI BsrI BstF5I Cfr13I DdeI DpnII DraI FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I