Restriction map for pF1KSDA1337
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AclI        AcsI        AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AvrII       BclI        
BlnI        BsaBI       BsiEI       BsmI        BspDI       
BspLU11I    Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FbaI        
FseI        HindIII     HpaI        MamI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SfuI        SgrAI       SnaBI       SphI        SspI        
SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        2406  GACGTC               GACGT^C
Acc65I        692  GGTACC               G^GTACC
AccIII       2402  TCCGGA               T^CCGGA
AflII         376  CTTAAG               C^TTAAG
AocI         2458  CCTGAGG              CC^T[ACGT]AGG
Asp718I       692  GGTACC               G^GTACC
AspI         1594  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         391  GGATCC               G^GATCC
BbvCI        3636  CCTCAGC              CCTCAGC(-5/-2)
BcgI         3167  GCAAGGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          376  CTTAAG               C^TTAAG
BglII        1052  AGATCT               A^GATCT
BsaWI        2402  TCCGGA               [AT]^CCGG[AT]
BseAI        2402  TCCGGA               T^CCGGA
BsiWI        3841  CGTACG               C^GTACG
Bsp1407I     2492  TGTACA               T^GTACA
BspEI        2402  TCCGGA               T^CCGGA
BspHI        1016  TCATGA               T^CATGA
BsrDI        1416  CATTGC               GCAATG(2/0)
BsrGI        2492  TGTACA               T^GTACA
Bsu36I       2458  CCTGAGG              CC^T[ACGT]AGG
DraIII        955  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3079  GACAACCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2458  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      2847  ATGCAT               ATGCA^T
KpnI          692  GGTACC               GGTAC^C
MroI         2402  TCCGGA               T^CCGGA
MseI          377  TTAA                 T^TAA
NheI         2449  GCTAGC               G^CTAGC
NsiI         2847  ATGCAT               ATGCA^T
NspI         1385  ACATGC               [GA]CATG^[CT]
PflFI        1594  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1016  TCATGA               T^CATGA
SalI         3372  GTCGAC               G^TCGAC
SbfI         2469  CCTGCAGG             CCTGCA^GG
ScaI         1538  AGTACT               AGT^ACT
SexAI        3761  ACCTGGT              A^CC[AT]GGT
SpeI         3009  ACTAGT               A^CTAGT
Sse8387I     2469  CCTGCAGG             CCTGCA^GG
SspBI        2492  TGTACA               T^GTACA
TfiI         4149  GATTC                G^A[AT]TC
Tru9I         377  TTAA                 T^TAA
Tth111I      1594  GACGATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII       1937  ACGTGT               A^C[GA][CT]GT
             2057  ACGTGT              
BbeI          754  GGCGCC               GGCGC^C
             4032  GGCGCC              
BbrPI        1519  CACGTG               CAC^GTG
             3862  CACGTG              
BciVI        3015  GGATAC               GTATCC(6/5)
             1532  GTATCC              
BlpI          675  GCTGAGC              GC^T[ACGT]AGC
             2871  GCTTAGC             
Bpu1102I      675  GCTGAGC              GC^T[ACGT]AGC
             2871  GCTTAGC             
BssHII        777  GCGCGC               G^CGCGC
              841  GCGCGC              
BstEII       1278  GGTCACC              G^GT[ACGT]ACC
             3666  GGTGACC             
BstPI        1278  GGTCACC              G^GT[ACGT]ACC
             3666  GGTGACC             
CelII         675  GCTGAGC              GC^T[ACGT]AGC
             2871  GCTTAGC             
EcoO65I      1278  GGTCACC              G^GT[ACGT]ACC
             3666  GGTGACC             
HincII       3058  GTCAAC               GT[CT]^[GA]AC
             3372  GTCGAC              
HindII       3058  GTCAAC               GT[CT]^[GA]AC
             3372  GTCGAC              
KasI          754  GGCGCC               G^GCGCC
             4032  GGCGCC              
NarI          754  GGCGCC               GG^CGCC
             4032  GGCGCC              
PflMI        1074  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGTACTTGG         
PmaCI        1519  CACGTG               CAC^GTG
             3862  CACGTG              
PmlI         1519  CACGTG               CAC^GTG
             3862  CACGTG              
SfiI         1290  GGCCATGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3585  GGCCGCGGTGGCC       
SfoI          754  GGCGCC               GGC^GCC
             4032  GGCGCC              
SmaI         3099  CCCGGG               CCC^GGG
             3300  CCCGGG              
StuI          341  AGGCCT               AGG^CCT
             3455  AGGCCT              
Tsp509I      1900  AATT                 ^AATT
             3144  AATT                
Van91I       1074  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGTACTTGG         
XmaCI        3099  CCCGGG               C^CCGGG
             3300  CCCGGG              
XmaI         3099  CCCGGG               C^CCGGG
             3300  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SacII       
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XcmI        XhoII       
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