Restriction map for pF1KSDA1325
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BclI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HaeIII      HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII         718  CGCG                 CG^CG
AflII         699  CTTAAG               C^TTAAG
Alw44I        632  GTGCAC               G^TGCAC
AlwI          803  GGATC                GGATC(4/5)
ApaLI         632  GTGCAC               G^TGCAC
AspHI         632  GTGCAC               G[AT]GC[AT]^C
AvaI          535  CTCGGG               C^[CT]CG[GA]G
AvaII         314  GGACC                G^G[AT]CC
BbsI          917  GTCTTC               GAAGAC(2/6)
BciVI         675  GGATAC               GTATCC(6/5)
BcnI          864  CCCGG                CC^[GC]GG
BfrI          699  CTTAAG               C^TTAAG
BpmI          619  CTGGAG               CTGGAG(16/14)
BpuAI         917  GTCTTC               GAAGAC(2/6)
BsiHKAI       632  GTGCAC               G[AT]GC[AT]^C
BsmI          591  GCATTC               GAATGC(1/-1)
BsoBI         535  CTCGGG               C^[CT]CG[GA]G
BssSI         745  CTCGTG               CACGAG(-5/-1)
BstUI         718  CGCG                 CG^CG
Cfr13I        314  GGACC                G^G[ACGT]CC
FauI          994  CCCGC                CCCGC(4/6)
HgaI          325  GACGC                GACGC(5/10)
HindIII       977  AAGCTT               A^AGCTT
HphI          938  TCACC                GGTGA(8/7)
MaeII         725  ACGT                 A^CGT
MaeIII        850  GTCAC                ^GT[ACGT]AC
MspA1I        401  CAGCTG               C[AC]G^C[GT]G
MvnI          718  CGCG                 CG^CG
NciI          864  CCCGG                CC^[GC]GG
NspI          601  GCATGT               [GA]CATG^[CT]
PleI          622  GAGTC                GAGTC(4/5)
PstI          691  CTGCAG               CTGCA^G
PvuII         401  CAGCTG               CAG^CTG
SapI          419  GAAGAGC              GCTCTTC(1/4)
Sau96I        314  GGACC                G^G[ACGT]CC
SfcI          691  CTGCAG               C^T[GA][CT]AG
Tsp45I        850  GTCAC                ^GT[GC]AC
XbaI          321  TCTAGA               T^CTAGA
XcmI          627  CCAGGGTGCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          297  GTAC                 GT^AC
              648  GTAC                
BanII         559  GAGCCC               G[GA]GC[CT]^C
              640  GGGCTC              
BsmAI         170  GAGAC                GTCTC(1/5)
              860  GTCTC               
DdeI           53  CTCAG                C^T[ACGT]AG
              329  CTGAG               
EarI          211  GAAGAG               CTCTTC(1/4)
              419  GAAGAG              
EcoT14I       554  CCAAGG               C^C[AT][AT]GG
              563  CCAAGG              
HapII         669  CCGG                 C^CGG
              865  CCGG                
HincII          6  GTTGAC               GT[CT]^[GA]AC
              197  GTCAAC              
HindII          6  GTTGAC               GT[CT]^[GA]AC
              197  GTCAAC              
HpaII         669  CCGG                 C^CGG
              865  CCGG                
Ksp632I       211  GAAGAG               CTCTTC(1/4)
              419  GAAGAG              
MslI          831  CATCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              885  CATGGCAATG          
MspI          669  CCGG                 C^CGG
              865  CCGG                
NlaIV         313  GGGACC               GG[ACGT]^[ACGT]CC
              558  GGAGCC              
RsaI          297  GTAC                 GT^AC
              648  GTAC                
SmlI          699  CTTAAG               C^T[CT][GA]AG
              734  CTCAAG              
StyI          554  CCAAGG               C^C[AT][AT]GG
              563  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BfaI        BmyI        BsaJI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
DpnII       EcoRII      Fnu4HI      FokI        HinfI       
ItaI        MaeI        MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      Sau3AI      
ScrFI       SfaNI       TfiI        Tru9I       TseI        
Tsp509I     TspRI       
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