Restriction map for pF1KSDA1293
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tru9I       Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        113  GGTACC               G^GTACC
AccII        1232  CGCG                 CG^CG
AcsI          213  GAATTC               [GA]^AATT[CT]
Alw44I        106  GTGCAC               G^TGCAC
ApaLI         106  GTGCAC               G^TGCAC
ApoI          213  GAATTC               [GA]^AATT[CT]
Asp718I       113  GGTACC               G^GTACC
BcgI          175  CGAGCCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI          206  GAGACC               GGTCTC(1/5)
BsmI          397  GCATTC               GAATGC(1/-1)
Bsp1407I      839  TGTACA               T^GTACA
BsrDI         740  GCAATG               GCAATG(2/0)
BsrFI         961  ACCGGC               [GA]^CCGG[CT]
BsrGI         839  TGTACA               T^GTACA
BssHII       1231  GCGCGC               G^CGCGC
BstUI        1232  CGCG                 CG^CG
BstXI         836  CCATGTACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        961  ACCGGC               [GA]^CCGG[CT]
EarI          673  CTCTTC               CTCTTC(1/4)
EcoRI         213  GAATTC               G^AATTC
EcoT14I       418  CCAAGG               C^C[AT][AT]GG
HaeII        1104  GGCGCT               [GA]GCGC^[CT]
HindIII       485  AAGCTT               A^AGCTT
HphI         1098  GGTGA                GGTGA(8/7)
KpnI          113  GGTACC               GGTAC^C
Ksp632I       673  CTCTTC               CTCTTC(1/4)
MvnI         1232  CGCG                 CG^CG
NspI          607  GCATGT               [GA]CATG^[CT]
PaeR7I        173  CTCGAG               C^TCGAG
PflMI        1167  CCACATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         999  CAGCTG               CAG^CTG
SacI          670  GAGCTC               GAGCT^C
SapI          672  GCTCTTC              GCTCTTC(1/4)
SspBI         839  TGTACA               T^GTACA
StyI          418  CCAAGG               C^C[AT][AT]GG
Tsp45I        958  GTGAC                ^GT[GC]AC
Van91I       1167  CCACATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          173  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BanII         176  GAGCCC               G[GA]GC[CT]^C
              670  GAGCTC              
BbsI         1153  GAAGAC               GAAGAC(2/6)
               34  GTCTTC              
BglII          22  AGATCT               A^GATCT
              542  AGATCT              
BpuAI        1153  GAAGAC               GAAGAC(2/6)
               34  GTCTTC              
BsmAI         206  GAGAC                GTCTC(1/5)
              440  GTCTC               
DraII         338  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              947  GGGACCC             
EcoO109I      338  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              947  GGGACCC             
MaeIII        958  GTGAC                ^GT[ACGT]AC
             1160  GTTAC               
MslI          837  CATGTACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              871  CACGCCAATG          
PpuMI         338  AGGTCCT              [GA]G^G[AT]CC[CT]
              947  GGGACCC             
PstI          679  CTGCAG               CTGCA^G
              830  CTGCAG              
SmaI           65  CCCGGG               CCC^GGG
             1094  CCCGGG              
SmlI          173  CTCGAG               C^T[CT][GA]AG
              331  CTCAAG              
TaqI          174  TCGA                 T^CGA
              668  TCGA                
TthHB8I       174  TCGA                 T^CGA
              668  TCGA                
XcmI           46  CCAGCCCCCTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              118  CCAGTCCTGGCCTGG     
XmaCI          65  CCCGGG               C^CCGGG
             1094  CCCGGG              
XmaI           65  CCCGGG               C^CCGGG
             1094  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TfiI        TseI        Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com