Restriction map for pF1KSDA1293
 (Linear) (Six-base) MAPSORT of: KIAA1293.seq  Check: 7257  from: 1  to: 1257



KIAA1293.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

Acc65I G'GTAC_C

Cuts at:      0     113    1257 
   Size:        113    1144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646    1075    1257 
   Size:        646     429     182

  Fragments arranged by size: 

                 646     429     182
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     893    1257 
   Size:        893     364
 
AlwNI CAG_nnn'CTG

Cuts at:      0     714    1020    1053    1257 
   Size:        714     306      33     204

  Fragments arranged by size: 

                 714     306     204      33
 
ApaLI G'TGCA_C

Cuts at:      0     106    1257 
   Size:        106    1151
 
ApoI r'AATT_y

Cuts at:      0     213    1257 
   Size:        213    1044
 
AvaI C'yCGr_G

Cuts at:      0      65     173    1094    1257 
   Size:         65     108     921     163

  Fragments arranged by size: 

                 921     163     108      65
 
BanI G'GyrC_C

Cuts at:      0      60     113     149     299    1257 
   Size:         60      53      36     150     958

  Fragments arranged by size: 

                 958     150      60      53      36
 
BanII G_rGCy'C

Cuts at:      0     180     674    1257 
   Size:        180     494     583

  Fragments arranged by size: 

                 583     494     180
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      27    1160    1257 
   Size:         27    1133      97

  Fragments arranged by size: 

                1133      97      27
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     164     198    1257 
   Size:        164      34    1059

  Fragments arranged by size: 

                1059     164      34
 
BglII A'GATC_T

Cuts at:      0      22     542    1257 
   Size:         22     520     715

  Fragments arranged by size: 

                 715     520      22
 
Bme1580I G_kGCm'C

Cuts at:      0      63     110     141     302    1257 
   Size:         63      47      31     161     955

  Fragments arranged by size: 

                 955     161      63      47      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      66     112     947    1257 
   Size:         66      46     835     310

  Fragments arranged by size: 

                 835     310      66      46
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     427     916    1019    1257 
   Size:        364      63     489     103     238

  Fragments arranged by size: 

                 489     364     238     103      63
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316    1257 
   Size:        316     941
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     200    1257 
   Size:        200    1057
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     177     712    1129    1257 
   Size:        177     535     417     128

  Fragments arranged by size: 

                 535     417     177     128
 
BseYI C'CCAG_C

Cuts at:      0      78    1209    1257 
   Size:         78    1131      48

  Fragments arranged by size: 

                1131      78      48
 
BsiHKAI G_wGCw'C

Cuts at:      0     110     674     872    1257 
   Size:        110     564     198     385

  Fragments arranged by size: 

                 564     385     198     110
 
BsmI GAATG_Cn'

Cuts at:      0     397    1257 
   Size:        397     860
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     110     141     180     302     674     872    1257 
   Size:         63      47      31      39     122     372     198     385

  Fragments arranged by size: 

                 385     372     198     122      63      47      39      31
            
 
BsrDI GCAATG_nn'

Cuts at:      0     747    1257 
   Size:        747     510
 
BsrFI r'CCGG_y

Cuts at:      0     961    1257 
   Size:        961     296
 
BsrGI T'GTAC_A

Cuts at:      0     839    1257 
   Size:        839     418
 
BssHII G'CGCG_C

Cuts at:      0    1231    1257 
   Size:       1231      26
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    1257 
   Size:        843     414
 
BstYI r'GATC_y

Cuts at:      0      22     542     747     887    1049    1122    1257 
   Size:         22     520     205     140     162      73     135

  Fragments arranged by size: 

                 520     205     162     140     135      73      22
 
BtsI GCAGTG_nn'

Cuts at:      0    1139    1257 
   Size:       1139     118
 
EarI CTCTTCn'nnn_

Cuts at:      0     679    1257 
   Size:        679     578
 
EcoICRI GAG'CTC

Cuts at:      0     672    1257 
   Size:        672     585
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     427     646     916    1019    1075    1257 
   Size:        364      63     219     270     103      56     182

  Fragments arranged by size: 

                 364     270     219     182     103      63      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     948    1257 
   Size:        339     609     309

  Fragments arranged by size: 

                 609     339     309
 
EcoRI G'AATT_C

Cuts at:      0     213    1257 
   Size:        213    1044
 
HaeII r_GCGC'y

Cuts at:      0    1108    1257 
   Size:       1108     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     675     707    1257 
   Size:        675      32     550

  Fragments arranged by size: 

                 675     550      32
 
HindIII A'AGCT_T

Cuts at:      0     485    1257 
   Size:        485     772
 
KpnI G_GTAC'C

Cuts at:      0     117    1257 
   Size:        117    1140
 
MslI CAynn'nnrTG

Cuts at:      0     841     875    1257 
   Size:        841      34     382

  Fragments arranged by size: 

                 841     382      34
 
MspA1I CmG'CkG

Cuts at:      0      14     143     447    1001    1257 
   Size:         14     129     304     554     256

  Fragments arranged by size: 

                 554     304     256     129      14
 
NspI r_CATG'y

Cuts at:      0     611    1257 
   Size:        611     646
 
PasI CC'CwG_GG

Cuts at:      0     735    1257 
   Size:        735     522
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1173    1257 
   Size:       1173      84
 
PfoI T'CCnGG_A

Cuts at:      0     341     401     599     983    1257 
   Size:        341      60     198     384     274

  Fragments arranged by size: 

                 384     341     274     198      60
 
PpuMI rG'GwC_Cy

Cuts at:      0     339     948    1257 
   Size:        339     609     309

  Fragments arranged by size: 

                 609     339     309
 
PspXI vC'TCGA_Gb

Cuts at:      0     173    1257 
   Size:        173    1084
 
PstI C_TGCA'G

Cuts at:      0     683     834    1257 
   Size:        683     151     423

  Fragments arranged by size: 

                 683     423     151
 
PvuII CAG'CTG

Cuts at:      0    1001    1257 
   Size:       1001     256
 
SacI G_AGCT'C

Cuts at:      0     674    1257 
   Size:        674     583
 
SanDI GG'GwC_CC

Cuts at:      0     948    1257 
   Size:        948     309
 
SapI GCTCTTCn'nnn_

Cuts at:      0     679    1257 
   Size:        679     578
 
SfcI C'TryA_G

Cuts at:      0     679     824     830    1257 
   Size:        679     145       6     427

  Fragments arranged by size: 

                 679     427     145       6
 
SmaI CCC'GGG

Cuts at:      0      67    1096    1257 
   Size:         67    1029     161

  Fragments arranged by size: 

                1029     161      67
 
SmlI C'TyrA_G

Cuts at:      0     173     331    1257 
   Size:        173     158     926

  Fragments arranged by size: 

                 926     173     158
 
StyI C'CwwG_G

Cuts at:      0     418    1257 
   Size:        418     839
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     317    1257 
   Size:        317     940
 
TatI w'GTAC_w

Cuts at:      0     347     791     839    1257 
   Size:        347     444      48     418

  Fragments arranged by size: 

                 444     418     347      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      53     125    1257 
   Size:         53      72    1132

  Fragments arranged by size: 

                1132      72      53
 
XhoI C'TCGA_G

Cuts at:      0     173    1257 
   Size:        173    1084
 
XmaI C'CCGG_G

Cuts at:      0      65    1094    1257 
   Size:         65    1029     163

  Fragments arranged by size: 

                1029     163      65
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbsI     BcgI    BglII Bme1580I     BmrI     BpmI    BpuEI
     BsaI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrFI
    BsrGI   BssHII    BstXI    BstYI     BtsI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I  HindIII     KpnI     MslI   MspA1I
     NspI     PasI    PflMI     PfoI    PpuMI    PspXI     PstI    PvuII
     SacI    SanDI     SapI     SfcI     SmaI     SmlI     StyI    TaqII
     TatI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com