Restriction map for
pF1KSDA1290
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# NO CUT ENZYMES ( 82 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BfrI BlnI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SfiI SfuI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 67 GACGTC GACGT^C
Acc65I 1267 GGTACC G^GTACC
AflIII 2056 ACGTGT A^C[GA][CT]GT
Alw44I 1273 GTGCAC G^TGCAC
ApaLI 1273 GTGCAC G^TGCAC
Asp718I 1267 GGTACC G^GTACC
AspI 1471 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1355 CCCGGG C^[CT]CG[GA]G
BbeI 584 GGCGCC GGCGC^C
BcgI 2432 GCAACTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 733 CTAG C^TAG
BsoBI 1355 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1538 TGTACA T^GTACA
BspHI 2876 TCATGA T^CATGA
BsrDI 2366 GCAATG GCAATG(2/0)
BsrGI 1538 TGTACA T^GTACA
BssHII 2892 GCGCGC G^CGCGC
BstEII 382 GGTCACC G^GT[ACGT]ACC
BstPI 382 GGTCACC G^GT[ACGT]ACC
EcoO65I 382 GGTCACC G^GT[ACGT]ACC
HaeII 584 GGCGCC [GA]GCGC^[CT]
KasI 584 GGCGCC G^GCGCC
KpnI 1267 GGTACC GGTAC^C
MaeI 733 CTAG C^TAG
MseI 1847 TTAA T^TAA
NarI 584 GGCGCC GG^CGCC
NdeI 2904 CATATG CA^TATG
PflFI 1471 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2876 TCATGA T^CATGA
SbfI 1614 CCTGCAGG CCTGCA^GG
SfoI 584 GGCGCC GGC^GCC
SgrAI 1486 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1355 CCCGGG CCC^GGG
Sse8387I 1614 CCTGCAGG CCTGCA^GG
SspBI 1538 TGTACA T^GTACA
StuI 3008 AGGCCT AGG^CCT
Tru9I 1847 TTAA T^TAA
Tth111I 1471 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1355 CCCGGG C^CCGGG
XmaI 1355 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AocI 1789 CCTGAGG CC^T[ACGT]AGG
2886 CCTGAGG
ApaI 2319 GGGCCC GGGCC^C
2636 GGGCCC
BamHI 659 GGATCC G^GATCC
1784 GGATCC
BbrPI 1969 CACGTG CAC^GTG
2476 CACGTG
BbsI 754 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BbvCI 9 GCTGAGG CCTCAGC(-5/-2)
2544 GCTGAGG
BciVI 2988 GGATAC GTATCC(6/5)
2584 GTATCC
BclI 455 TGATCA T^GATCA
2777 TGATCA
BglI 797 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1487 GCCGGCGTGGC
BglII 944 AGATCT A^GATCT
2753 AGATCT
BlpI 256 GCTCAGC GC^T[ACGT]AGC
1977 GCTCAGC
Bpu1102I 256 GCTCAGC GC^T[ACGT]AGC
1977 GCTCAGC
BpuAI 754 GAAGAC GAAGAC(2/6)
1119 GAAGAC
BsaAI 1969 CACGTG [CT]AC^GT[GA]
2476 CACGTG
BsaI 676 GAGACC GGTCTC(1/5)
1225 GAGACC
BsiEI 1343 CGACCG CG[GA][CT]^CG
1647 CGACCG
BstAPI 1763 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2490 GCAGATCCTGC
Bsu36I 1789 CCTGAGG CC^T[ACGT]AGG
2886 CCTGAGG
CelII 256 GCTCAGC GC^T[ACGT]AGC
1977 GCTCAGC
Eco81I 1789 CCTGAGG CC^T[ACGT]AGG
2886 CCTGAGG
FbaI 455 TGATCA T^GATCA
2777 TGATCA
HgaI 1192 GACGC GACGC(5/10)
1040 GCGTC
MslI 1381 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1965 CATCCACGTG
NaeI 1487 GCCGGC GCC^GGC
2486 GCCGGC
NcoI 2159 CCATGG C^CATGG
2301 CCATGG
NgoMIV 1487 GCCGGC G^CCGGC
2486 GCCGGC
PmaCI 1969 CACGTG CAC^GTG
2476 CACGTG
PmlI 1969 CACGTG CAC^GTG
2476 CACGTG
SmlI 303 CTCAAG C^T[CT][GA]AG
829 CTCAAG
Tsp509I 326 AATT ^AATT
1635 AATT
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII