Restriction map for pF1KSDA1279
 (Linear) (Six-base) MAPSORT of: KIAA1279.seq  Check: 5243  from: 1  to: 1863



KIAA1279.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AccI GT'mk_AC

Cuts at:      0     650    1376    1863 
   Size:        650     726     487

  Fragments arranged by size: 

                 726     650     487
 
Acc65I G'GTAC_C

Cuts at:      0     380    1863 
   Size:        380    1483
 
AclI AA'CG_TT

Cuts at:      0       8    1863 
   Size:          8    1855
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     899     905    1561    1863 
   Size:        587     312       6     656     302

  Fragments arranged by size: 

                 656     587     312     302       6
 
AgeI A'CCGG_T

Cuts at:      0    1099    1863 
   Size:       1099     764
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42    1863 
   Size:         42    1821
 
AlwNI CAG_nnn'CTG

Cuts at:      0     660     812    1863 
   Size:        660     152    1051

  Fragments arranged by size: 

                1051     660     152
 
ApaI G_GGCC'C

Cuts at:      0     257    1863 
   Size:        257    1606
 
ApoI r'AATT_y

Cuts at:      0      34    1701    1729    1863 
   Size:         34    1667      28     134

  Fragments arranged by size: 

                1667     134      34      28
 
AvaI C'yCGr_G

Cuts at:      0     116     198     247     301    1863 
   Size:        116      82      49      54    1562

  Fragments arranged by size: 

                1562     116      82      54      49
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     420     453    1863 
   Size:        420      33    1410

  Fragments arranged by size: 

                1410     420      33
 
BamHI G'GATC_C

Cuts at:      0    1488    1863 
   Size:       1488     375
 
BanI G'GyrC_C

Cuts at:      0     202     380    1863 
   Size:        202     178    1483

  Fragments arranged by size: 

                1483     202     178
 
BanII G_rGCy'C

Cuts at:      0     248     257    1367    1863 
   Size:        248       9    1110     496

  Fragments arranged by size: 

                1110     496     248       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     875    1807    1863 
   Size:        875     932      56

  Fragments arranged by size: 

                 932     875      56
 
BbvCI CC'TCA_GC

Cuts at:      0     233    1863 
   Size:        233    1630
 
BfrBI ATG'CAT

Cuts at:      0    1338    1863 
   Size:       1338     525
 
BglI GCCn_nnn'nGGC

Cuts at:      0     252    1863 
   Size:        252    1611
 
BglII A'GATC_T

Cuts at:      0     854    1863 
   Size:        854    1009
 
Bme1580I G_kGCm'C

Cuts at:      0     257    1863 
   Size:        257    1606
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     554    1863 
   Size:        554    1309
 
Bpu10I CC'TnA_GC

Cuts at:      0     233    1483    1595    1863 
   Size:        233    1250     112     268

  Fragments arranged by size: 

                1250     268     233     112
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     629     736    1170    1863 
   Size:        629     107     434     693

  Fragments arranged by size: 

                 693     629     434     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1146    1568    1863 
   Size:       1146     422     295

  Fragments arranged by size: 

                1146     422     295
 
BsaWI w'CCGG_w

Cuts at:      0      81    1099    1863 
   Size:         81    1018     764

  Fragments arranged by size: 

                1018     764      81
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346    1312    1342    1863 
   Size:        316      30     966      30     521

  Fragments arranged by size: 

                 966     521     316      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     358     527    1075    1863 
   Size:        340      18     169     548     788

  Fragments arranged by size: 

                 788     548     340     169      18
 
BseYI C'CCAG_C

Cuts at:      0     263    1863 
   Size:        263    1600
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    1108    1863 
   Size:        455     653     755

  Fragments arranged by size: 

                 755     653     455
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     722    1284    1863 
   Size:        350     372     562     579

  Fragments arranged by size: 

                 579     562     372     350
 
BsmI GAATG_Cn'

Cuts at:      0     975    1070    1300    1863 
   Size:        975      95     230     563

  Fragments arranged by size: 

                 975     563     230      95
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1317    1660    1863 
   Size:       1317     343     203

  Fragments arranged by size: 

                1317     343     203
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     257     350     722    1284    1367    1863 
   Size:        248       9      93     372     562      83     496

  Fragments arranged by size: 

                 562     496     372     248      93      83       9
 
BspEI T'CCGG_A

Cuts at:      0      81    1863 
   Size:         81    1782
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     368    1863 
   Size:        368    1495
 
BsrBI CCG'CTC

Cuts at:      0     177     271    1863 
   Size:        177      94    1592

  Fragments arranged by size: 

                1592     177      94
 
BsrDI GCAATG_nn'

Cuts at:      0    1469    1863 
   Size:       1469     394
 
BsrFI r'CCGG_y

Cuts at:      0     205     228     383    1099    1863 
   Size:        205      23     155     716     764

  Fragments arranged by size: 

                 764     716     205     155      23
 
BstEII G'GTnAC_C

Cuts at:      0     208    1863 
   Size:        208    1655
 
BstYI r'GATC_y

Cuts at:      0     854    1415    1488    1680    1863 
   Size:        854     561      73     192     183

  Fragments arranged by size: 

                 854     561     192     183      73
 
Bsu36I CC'TnA_GG

Cuts at:      0     161     182    1760    1863 
   Size:        161      21    1578     103

  Fragments arranged by size: 

                1578     161     103      21
 
BtgI C'CryG_G

Cuts at:      0      13    1863 
   Size:         13    1850
 
DraIII CAC_nnn'GTG

Cuts at:      0     354    1863 
   Size:        354    1509
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     566    1127    1561    1863 
   Size:        125     441     561     434     302

  Fragments arranged by size: 

                 561     441     434     302     125
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     899     905    1561    1863 
   Size:        587     312       6     656     302

  Fragments arranged by size: 

                 656     587     312     302       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     253    1863 
   Size:        253    1610
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     595     810    1633    1863 
   Size:        595     215     823     230

  Fragments arranged by size: 

                 823     595     230     215
 
FspI TGC'GCA

Cuts at:      0     376    1863 
   Size:        376    1487
 
HaeII r_GCGC'y

Cuts at:      0      49     116     124     145    1863 
   Size:         49      67       8      21    1718

  Fragments arranged by size: 

                1718      67      49      21       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     388     420    1006    1038    1105    1137 
   Size:        316      32      40      32     586      32      67      32

Cuts at:   1137    1797    1829    1863 
   Size:        660      32      34

  Fragments arranged by size: 

                 660     586     316      67      40      34      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1407    1863 
   Size:       1407     456
 
KpnI G_GTAC'C

Cuts at:      0     384    1863 
   Size:        384    1479
 
MspA1I CmG'CkG

Cuts at:      0     823    1863 
   Size:        823    1040
 
NaeI GCC'GGC

Cuts at:      0     230    1863 
   Size:        230    1633
 
NgoMIV G'CCGG_C

Cuts at:      0     228    1863 
   Size:        228    1635
 
NsiI A_TGCA'T

Cuts at:      0    1340    1863 
   Size:       1340     523
 
NspI r_CATG'y

Cuts at:      0    1438    1863 
   Size:       1438     425
 
PasI CC'CwG_GG

Cuts at:      0     219    1863 
   Size:        219    1644
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     219     660    1863 
   Size:        219     441    1203

  Fragments arranged by size: 

                1203     441     219
 
PspOMI G'GGCC_C

Cuts at:      0     253    1863 
   Size:        253    1610
 
PstI C_TGCA'G

Cuts at:      0    1128    1680    1863 
   Size:       1128     552     183

  Fragments arranged by size: 

                1128     552     183
 
PvuII CAG'CTG

Cuts at:      0     823    1863 
   Size:        823    1040
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1561    1863 
   Size:       1561     302
 
ScaI AGT'ACT

Cuts at:      0    1546    1863 
   Size:       1546     317
 
SexAI A'CCwGG_T

Cuts at:      0     350    1863 
   Size:        350    1513
 
SfcI C'TryA_G

Cuts at:      0     737    1124    1373    1676    1863 
   Size:        737     387     249     303     187

  Fragments arranged by size: 

                 737     387     303     249     187
 
SmaI CCC'GGG

Cuts at:      0     118     200    1863 
   Size:        118      82    1663

  Fragments arranged by size: 

                1663     118      82
 
SmlI C'TyrA_G

Cuts at:      0     644     715    1149    1863 
   Size:        644      71     434     714

  Fragments arranged by size: 

                 714     644     434      71
 
SrfI GCCC'GGGC

Cuts at:      0     118    1863 
   Size:        118    1745
 
TatI w'GTAC_w

Cuts at:      0     697    1544    1863 
   Size:        697     847     319

  Fragments arranged by size: 

                 847     697     319
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1312    1344    1863 
   Size:       1312      32     519

  Fragments arranged by size: 

                1312     519      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1692    1863 
   Size:       1692     171
 
XmaI C'CCGG_G

Cuts at:      0     116     198    1863 
   Size:        116      82    1665

  Fragments arranged by size: 

                1665     116      82
 
XmnI GAAnn'nnTTC

Cuts at:      0    1070    1613    1863 
   Size:       1070     543     250

  Fragments arranged by size: 

                1070     543     250
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AgeI     AlfI    AlwNI     ApaI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
    BfrBI     BglI    BglII Bme1580I     BplI   Bpu10I    BpuEI     BsaI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstEII    BstYI
   Bsu36I     BtgI   DraIII     EarI  Eco57MI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII     KpnI   MspA1I     NaeI   NgoMIV     NsiI
     NspI     PasI    PflMI   PspOMI     PstI    PvuII     SapI     ScaI
    SexAI     SfcI     SmaI     SmlI     SrfI     TatI     TstI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AhdI     AleI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BcgI    BciVI
     BclI     BlpI    BmgBI     BmrI     BmtI     BpmI    BsaAI    BsaBI
    BsaHI    BsiEI    BsiWI    BspHI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI    FspAI  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XhoI     ZraI
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