Restriction map for pF1KSDA1274
##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccI        AclI        AflII       Alw44I      
AocI        ApaLI       AscI        AseI        AsnI        
Asp700I     AviII       AvrII       BamHI       BbeI        
BbrPI       BfrI        BglII       BlnI        BsiWI       
BsmBI       BsmI        BspDI       BspLU11I    BsrDI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KspI        MfeI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
StuI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        794  GGTACC               G^GTACC
AccIII        623  TCCGGA               T^CCGGA
AcsI         2113  GAATTT               [GA]^AATT[CT]
AgeI         2513  ACCGGT               A^CCGGT
ApoI         2113  GAATTT               [GA]^AATT[CT]
Asp718I       794  GGTACC               G^GTACC
AspI         2191  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1983  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1202  GTATCC               GTATCC(6/5)
BclI          179  TGATCA               T^GATCA
BfaI          122  CTAG                 C^TAG
BsaAI         183  CACGTA               [CT]AC^GT[GA]
BsaBI        2238  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         623  TCCGGA               T^CCGGA
BsiEI        1207  CGACCG               CG[GA][CT]^CG
Bsp1407I      758  TGTACA               T^GTACA
BspEI         623  TCCGGA               T^CCGGA
BsrBI        2478  GAGCGG               CCGCTC(-3/-3)
BsrGI         758  TGTACA               T^GTACA
BssSI        2077  CTCGTG               CACGAG(-5/-1)
BstAPI       2283  GCAGGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII       2158  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
FbaI          179  TGATCA               T^GATCA
KpnI          794  GGTACC               GGTAC^C
MaeI          122  CTAG                 C^TAG
MamI         2238  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2354  ACGCGT               A^CGCGT
MroI          623  TCCGGA               T^CCGGA
NaeI          248  GCCGGC               GCC^GGC
NcoI          723  CCATGG               C^CATGG
NgoMIV        248  GCCGGC               G^CCGGC
PflFI        2191  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2513  ACCGGT               A^CCGGT
ScaI         2318  AGTACT               AGT^ACT
SexAI         278  ACCTGGT              A^CC[AT]GGT
SfiI         1929  GGCCCTGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          589  CCCGGG               CCC^GGG
SphI         1532  GCATGC               GCATG^C
SspBI         758  TGTACA               T^GTACA
TfiI         2343  GATTC                G^A[AT]TC
Tsp509I      2114  AATT                 ^AATT
Tth111I      2191  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         589  CCCGGG               C^CCGGG
XmaI          589  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII        1479  CGCG                 CG^CG
             2355  CGCG                
AflIII        239  ACACGT               A^C[GA][CT]GT
             2354  ACGCGT              
Aor51HI      2184  AGCGCT               AGC^GCT
             2327  AGCGCT              
BbsI          925  GTCTTC               GAAGAC(2/6)
             1825  GTCTTC              
BcgI         2541  CGAGCCCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1961  GCACTGGCTTCG        
BpuAI         925  GTCTTC               GAAGAC(2/6)
             1825  GTCTTC              
BspHI         564  TCATGA               T^CATGA
             1314  TCATGA              
BsrFI         248  GCCGGC               [GA]^CCGG[CT]
             2513  ACCGGT              
BsrI         1963  ACTGG                ACTGG(1/-1)
              840  CCAGT               
BstUI        1479  CGCG                 CG^CG
             2355  CGCG                
Cfr10I        248  GCCGGC               [GA]^CCGG[CT]
             2513  ACCGGT              
DrdI          847  GACGCCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1459  GACGATCTGGTC        
Eco47III     2184  AGCGCT               AGC^GCT
             2327  AGCGCT              
MaeII         184  ACGT                 A^CGT
              241  ACGT                
MseI         1301  TTAA                 T^TAA
             2450  TTAA                
MvnI         1479  CGCG                 CG^CG
             2355  CGCG                
NspI          741  GCATGT               [GA]CATG^[CT]
             1532  GCATGC              
PaeR7I        545  CTCGAG               C^TCGAG
             2539  CTCGAG              
PleI         2380  GACTC                GAGTC(4/5)
              825  GAGTC               
RcaI          564  TCATGA               T^CATGA
             1314  TCATGA              
SacI         1401  GAGCTC               GAGCT^C
             2074  GAGCTC              
Tru9I        1301  TTAA                 T^TAA
             2450  TTAA                
XcmI          956  CCAACCTGGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2003  CCACAACTGCGATGG     
XhoI          545  CTCGAG               C^TCGAG
             2539  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcyI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApaI        AspEI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eam1105I    EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
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