Restriction map for pF1KSDA1274
 (Linear) (Six-base) MAPSORT of: KIAA1274.seq  Check: 951  from: 1  to: 2568



KIAA1274.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     363    2237    2568 
   Size:        363    1874     331

  Fragments arranged by size: 

                1874     363     331
 
Acc65I G'GTAC_C

Cuts at:      0     794    2568 
   Size:        794    1774
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     912    1762    1812    2382    2568 
   Size:        912     850      50     570     186

  Fragments arranged by size: 

                 912     850     570     186      50
 
AfeI AGC'GCT

Cuts at:      0    2186    2329    2568 
   Size:       2186     143     239

  Fragments arranged by size: 

                2186     239     143
 
AflIII A'CryG_T

Cuts at:      0     239    2354    2568 
   Size:        239    2115     214

  Fragments arranged by size: 

                2115     239     214
 
AgeI A'CCGG_T

Cuts at:      0    2513    2568 
   Size:       2513      55
 
AhdI GACnn_n'nnGTC

Cuts at:      0     531     592    1246    2568 
   Size:        531      61     654    1322

  Fragments arranged by size: 

                1322     654     531      61
 
AleI CACnn'nnGTG

Cuts at:      0     998    2568 
   Size:        998    1570
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     142    1894    2425    2568 
   Size:        142    1752     531     143

  Fragments arranged by size: 

                1752     531     143     142
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1024    1250    1355    1414    1847    1923    1963    2405 
   Size:       1024     226     105      59     433      76      40     442

Cuts at:   2405    2568 
   Size:        163

  Fragments arranged by size: 

                1024     442     433     226     163     105      76      59
                  40
 
ApaI G_GGCC'C

Cuts at:      0     360    1700    1783    2568 
   Size:        360    1340      83     785

  Fragments arranged by size: 

                1340     785     360      83
 
ApoI r'AATT_y

Cuts at:      0    2113    2568 
   Size:       2113     455
 
AvaI C'yCGr_G

Cuts at:      0     545     589     814    1898    2056    2467    2539    2554 
   Size:        545      44     225    1084     158     411      72      15

Cuts at:   2554    2568 
   Size:         14

  Fragments arranged by size: 

                1084     545     411     225     158      72      44      15
                  14
 
BanI G'GyrC_C

Cuts at:      0      43     337     794     976    1092    1524    2156    2568 
   Size:         43     294     457     182     116     432     632     412

  Fragments arranged by size: 

                 632     457     432     412     294     182     116      43
            
 
BanII G_rGCy'C

Cuts at:      0     360     389     707    1219    1405    1447    1700    1783 
   Size:        360      29     318     512     186      42     253      83

Cuts at:   1783    2078    2546    2568 
   Size:        295     468      22

  Fragments arranged by size: 

                 512     468     360     318     295     253     186      83
                  42      29      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     918    1818    2568 
   Size:        918     900     750

  Fragments arranged by size: 

                 918     900     750
 
BbvCI CC'TCA_GC

Cuts at:      0    1984    2568 
   Size:       1984     584
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1950    1984    2530    2564    2568 
   Size:       1950      34     546      34       4

  Fragments arranged by size: 

                1950     546      34      34       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1213    2568 
   Size:       1213    1355
 
BclI T'GATC_A

Cuts at:      0     179    2568 
   Size:        179    2389
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65     941    1936    1988    2568 
   Size:         65     876     995      52     580

  Fragments arranged by size: 

                 995     876     580      65      52
 
BlpI GC'TnA_GC

Cuts at:      0     652    1399    1404    1483    2568 
   Size:        652     747       5      79    1085

  Fragments arranged by size: 

                1085     747     652      79       5
 
Bme1580I G_kGCm'C

Cuts at:      0     340     360     979    1097    1368    1529    1673    1700 
   Size:        340      20     619     118     271     161     144      27

Cuts at:   1700    1783    1896    2159    2568 
   Size:         83     113     263     409

  Fragments arranged by size: 

                 619     409     340     271     263     161     144     118
                 113      83      27      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     834    2568 
   Size:        834    1734
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     433     559     631     853    1294    1741    1750 
   Size:        424       9     126      72     222     441     447       9

Cuts at:   1750    1945    2389    2484    2494    2568 
   Size:        195     444      95      10      74

  Fragments arranged by size: 

                 447     444     441     424     222     195     126      95
                  74      72      10       9       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     269    1373    1984    2568 
   Size:        269    1104     611     584

  Fragments arranged by size: 

                1104     611     584     269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211    2568 
   Size:        211    2357
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      39     865    1473    1717    2197    2568 
   Size:         39     826     608     244     480     371

  Fragments arranged by size: 

                 826     608     480     371     244      39
 
BsaAI yAC'GTr

Cuts at:      0     185    2568 
   Size:        185    2383
 
BsaBI GATnn'nnATC

Cuts at:      0    2242    2568 
   Size:       2242     326
 
BsaHI Gr'CG_yC

Cuts at:      0     848    1601    2212    2568 
   Size:        848     753     611     356

  Fragments arranged by size: 

                 848     753     611     356
 
BsaWI w'CCGG_w

Cuts at:      0     623    1209    2513    2568 
   Size:        623     586    1304      55

  Fragments arranged by size: 

                1304     623     586      55
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     742     772     826     856    1639    1669    2568 
   Size:        742      30      54      30     783      30     899

  Fragments arranged by size: 

                 899     783     742      54      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     214     766    1623    1660    1918    2083    2086    2568 
   Size:        214     552     857      37     258     165       3     482

  Fragments arranged by size: 

                 857     552     482     258     214     165      37       3
            
 
BseYI C'CCAG_C

Cuts at:      0      23     333     412     647     877     915    1013    1218 
   Size:         23     310      79     235     230      38      98     205

Cuts at:   1218    1553    1558    1736    2457    2568 
   Size:        335       5     178     721     111

  Fragments arranged by size: 

                 721     335     310     235     230     205     178     111
                  98      79      38      23       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     536    2158    2232    2568 
   Size:        397     139    1622      74     336

  Fragments arranged by size: 

                1622     397     336     139      74
 
BsiEI CG_ry'CG

Cuts at:      0    1210    2568 
   Size:       1210    1358
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     275    1405    2078    2568 
   Size:        121     154    1130     673     490

  Fragments arranged by size: 

                1130     673     490     154     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     275     340     360     389     707     979    1097 
   Size:        121     154      65      20      29     318     272     118

Cuts at:   1097    1219    1368    1405    1447    1529    1673    1700    1783 
   Size:        122     149      37      42      82     144      27      83

Cuts at:   1783    1896    2078    2159    2546    2568 
   Size:        113     182      81     387      22

  Fragments arranged by size: 

                 387     318     272     182     154     149     144     122
                 121     118     113      83      82      81      65      42
                  37      29      27      22      20
 
BspEI T'CCGG_A

Cuts at:      0     623    2568 
   Size:        623    1945
 
BspHI T'CATG_A

Cuts at:      0     564    1314    2568 
   Size:        564     750    1254

  Fragments arranged by size: 

                1254     750     564
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     363    1813    2237    2568 
   Size:        363    1450     424     331

  Fragments arranged by size: 

                1450     424     363     331
 
BsrBI CCG'CTC

Cuts at:      0    2480    2568 
   Size:       2480      88
 
BsrFI r'CCGG_y

Cuts at:      0     248    2513    2568 
   Size:        248    2265      55

  Fragments arranged by size: 

                2265     248      55
 
BsrGI T'GTAC_A

Cuts at:      0     758    2568 
   Size:        758    1810
 
BssSI C'ACGA_G

Cuts at:      0    2077    2568 
   Size:       2077     491
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2289    2568 
   Size:       2289     279
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     165     583    1572    2568 
   Size:        165     418     989     996

  Fragments arranged by size: 

                 996     989     418     165
 
BstYI r'GATC_y

Cuts at:      0     209     632    2444    2568 
   Size:        209     423    1812     124

  Fragments arranged by size: 

                1812     423     209     124
 
BtgI C'CryG_G

Cuts at:      0     341     723     903    2568 
   Size:        341     382     180    1665

  Fragments arranged by size: 

                1665     382     341     180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1419    2026    2568 
   Size:       1419     607     542

  Fragments arranged by size: 

                1419     607     542
 
BtsI GCAGTG_nn'

Cuts at:      0      75    1005    1136    2568 
   Size:         75     930     131    1432

  Fragments arranged by size: 

                1432     930     131      75
 
DraIII CAC_nnn'GTG

Cuts at:      0    2163    2568 
   Size:       2163     405
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     853    1465    2568 
   Size:        853     612    1103

  Fragments arranged by size: 

                1103     853     612
 
EaeI y'GGCC_r

Cuts at:      0      11     617     716    1508    1989    2568 
   Size:         11     606      99     792     481     579

  Fragments arranged by size: 

                 792     606     579     481      99      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     778    1112    2353    2568 
   Size:        778     334    1241     215

  Fragments arranged by size: 

                1241     778     334     215
 
EcoICRI GAG'CTC

Cuts at:      0    1403    2076    2568 
   Size:       1403     673     492

  Fragments arranged by size: 

                1403     673     492
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     433     559     631     853     912    1294    1741 
   Size:        424       9     126      72     222      59     382     447

Cuts at:   1741    1750    1762    1812    1945    2382    2389    2484    2494 
   Size:          9      12      50     133     437       7      95      10

Cuts at:   2494    2568 
   Size:         74

  Fragments arranged by size: 

                 447     437     424     382     222     133     126      95
                  74      72      59      50      12      10       9       9
                   7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1453    1642    1766    1948    2371    2388    2499    2568 
   Size:       1453     189     124     182     423      17     111      69

  Fragments arranged by size: 

                1453     423     189     182     124     111      69      17
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      57     356     357     435     579     690    1411    1428 
   Size:         57     299       1      78     144     111     721      17

Cuts at:   1428    1569    1697    1779    1780    1844    1929    1953    2393 
   Size:        141     128      82       1      64      85      24     440

Cuts at:   2393    2568 
   Size:        175

  Fragments arranged by size: 

                 721     440     299     175     144     141     128     111
                  85      82      78      64      57      24      17       1
                   1
 
HaeII r_GCGC'y

Cuts at:      0    1264    1565    2188    2331    2568 
   Size:       1264     301     623     143     237

  Fragments arranged by size: 

                1264     623     301     237     143
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      69     101     517     549     578     610    1232    1264 
   Size:         69      32     416      32      29      32     622      32

Cuts at:   1264    1453    1485    2407    2439    2568 
   Size:        189      32     922      32     129

  Fragments arranged by size: 

                 922     622     416     189     129      69      32      32
                  32      32      32      32      29
 
KpnI G_GTAC'C

Cuts at:      0     798    2568 
   Size:        798    1770
 
MluI A'CGCG_T

Cuts at:      0    2354    2568 
   Size:       2354     214
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1230    1637    2568 
   Size:        824     406     407     931

  Fragments arranged by size: 

                 931     824     407     406
 
MscI TGG'CCA

Cuts at:      0     619     718    1510    2568 
   Size:        619      99     792    1058

  Fragments arranged by size: 

                1058     792     619      99
 
MslI CAynn'nnrTG

Cuts at:      0     998    1876    2407    2568 
   Size:        998     878     531     161

  Fragments arranged by size: 

                 998     878     531     161
 
MspA1I CmG'CkG

Cuts at:      0     651     932    1329    1355    1719    1740    1920    1979 
   Size:        651     281     397      26     364      21     180      59

Cuts at:   1979    1988    2142    2304    2568 
   Size:          9     154     162     264

  Fragments arranged by size: 

                 651     397     364     281     264     180     162     154
                  59      26      21       9
 
NaeI GCC'GGC

Cuts at:      0     250    2568 
   Size:        250    2318
 
NcoI C'CATG_G

Cuts at:      0     723    2568 
   Size:        723    1845
 
NgoMIV G'CCGG_C

Cuts at:      0     248    2568 
   Size:        248    2320
 
NspI r_CATG'y

Cuts at:      0     745    1536    2568 
   Size:        745     791    1032

  Fragments arranged by size: 

                1032     791     745
 
PasI CC'CwG_GG

Cuts at:      0    1098    1224    1572    2568 
   Size:       1098     126     348     996

  Fragments arranged by size: 

                1098     996     348     126
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     714    1923    1963    2568 
   Size:        714    1209      40     605

  Fragments arranged by size: 

                1209     714     605      40
 
PfoI T'CCnGG_A

Cuts at:      0     866    2568 
   Size:        866    1702
 
PpuMI rG'GwC_Cy

Cuts at:      0     435     690    1428    1953    2568 
   Size:        435     255     738     525     615

  Fragments arranged by size: 

                 738     615     525     435     255
 
PspOMI G'GGCC_C

Cuts at:      0     356    1696    1779    2568 
   Size:        356    1340      83     789

  Fragments arranged by size: 

                1340     789     356      83
 
PspXI vC'TCGA_Gb

Cuts at:      0    2539    2568 
   Size:       2539      29
 
PstI C_TGCA'G

Cuts at:      0     782     890    2303    2318    2536    2568 
   Size:        782     108    1413      15     218      32

  Fragments arranged by size: 

                1413     782     218     108      32      15
 
PvuII CAG'CTG

Cuts at:      0     651     932    1719    1740    1920    1979    2142    2304 
   Size:        651     281     787      21     180      59     163     162

Cuts at:   2304    2568 
   Size:        264

  Fragments arranged by size: 

                 787     651     281     264     180     163     162      59
                  21
 
SacI G_AGCT'C

Cuts at:      0    1405    2078    2568 
   Size:       1405     673     490

  Fragments arranged by size: 

                1405     673     490
 
ScaI AGT'ACT

Cuts at:      0    2320    2568 
   Size:       2320     248
 
SexAI A'CCwGG_T

Cuts at:      0     278    2568 
   Size:        278    2290
 
SfcI C'TryA_G

Cuts at:      0      61     778     789     886    1677    1791    2299    2314 
   Size:         61     717      11      97     791     114     508      15

Cuts at:   2314    2532    2568 
   Size:        218      36

  Fragments arranged by size: 

                 791     717     508     218     114      97      61      36
                  15      11
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1936    2568 
   Size:       1936     632
 
SmaI CCC'GGG

Cuts at:      0     591    2568 
   Size:        591    1977
 
SmlI C'TyrA_G

Cuts at:      0     226     545    2539    2568 
   Size:        226     319    1994      29

  Fragments arranged by size: 

                1994     319     226      29
 
SphI G_CATG'C

Cuts at:      0    1536    2568 
   Size:       1536    1032
 
StyI C'CwwG_G

Cuts at:      0     582     723    1565    1949    2046    2568 
   Size:        582     141     842     384      97     522

  Fragments arranged by size: 

                 842     582     522     384     141      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1620    2053    2568 
   Size:       1620     433     515

  Fragments arranged by size: 

                1620     515     433
 
TatI w'GTAC_w

Cuts at:      0     758    2318    2568 
   Size:        758    1560     250

  Fragments arranged by size: 

                1560     758     250
 
Tth111I GACn'n_nGTC

Cuts at:      0    2194    2568 
   Size:       2194     374
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     963    2010    2568 
   Size:        963    1047     558

  Fragments arranged by size: 

                1047     963     558
 
XhoI C'TCGA_G

Cuts at:      0     545    2539    2568 
   Size:        545    1994      29

  Fragments arranged by size: 

                1994     545      29
 
XmaI C'CCGG_G

Cuts at:      0     589    2568 
   Size:        589    1979
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI     ScaI    SexAI     SfcI     SfiI     SmaI     SmlI     SphI
     StyI    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BfrBI    BglII    BmgBI
     BmtI     BplI    BsiWI     BsmI    BsmBI    BsrDI   BssHII    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TstI     XbaI     XmnI     ZraI
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