Restriction map for pF1KSDA1264
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        AspI        
AviII       AvrII       BbeI        BbrPI       BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsgI        BsiWI       BspDI       
BsrBI       BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
CfoI        ClaI        CpoI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KspI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     Van91I      XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       1127  GGTACC               G^GTACC
AccII         357  CGCG                 CG^CG
AccIII       1445  TCCGGA               T^CCGGA
AflIII       1523  ACATGT               A^C[GA][CT]GT
AhdI         1873  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1034  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1056  GGGCCC               GGGCC^C
Asp700I      1288  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1127  GGTACC               G^GTACC
AspEI        1873  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1346  TGGCCA               TGG^CCA
BcgI          405  CGATACTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1487  TGATCA               T^GATCA
BsaAI         511  CACGTA               [CT]AC^GT[GA]
BsaI         1328  GAGACC               GGTCTC(1/5)
BsaWI        1445  TCCGGA               [AT]^CCGG[AT]
BseAI        1445  TCCGGA               T^CCGGA
BsmBI        1458  GAGACG               CGTCTC(1/5)
BsmI          980  GCATTC               GAATGC(1/-1)
Bsp1407I     1526  TGTACA               T^GTACA
BspEI        1445  TCCGGA               T^CCGGA
BspHI        2135  TCATGA               T^CATGA
BspLU11I     1523  ACATGT               A^CATGT
BspMI        2107  GCAGGT               ACCTGC(4/8)
BsrGI        1526  TGTACA               T^GTACA
BstAPI       1382  GCATCGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         357  CGCG                 CG^CG
BstXI         182  CCATCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          910  TTTAAA               TTT^AAA
DraIII        972  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1873  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         1487  TGATCA               T^GATCA
HincII       1519  GTTGAC               GT[CT]^[GA]AC
HindII       1519  GTTGAC               GT[CT]^[GA]AC
KpnI         1127  GGTACC               GGTAC^C
MaeII         512  ACGT                 A^CGT
MluNI        1346  TGGCCA               TGG^CCA
MroI         1445  TCCGGA               T^CCGGA
MscI         1346  TGGCCA               TGG^CCA
MvnI          357  CGCG                 CG^CG
NspI         1523  ACATGT               [GA]CATG^[CT]
PaeR7I        666  CTCGAG               C^TCGAG
PvuI          999  CGATCG               CGAT^CG
RcaI         2135  TCATGA               T^CATGA
SspBI        1526  TGTACA               T^GTACA
StuI          536  AGGCCT               AGG^CCT
Tsp509I       698  AATT                 ^AATT
XhoI          666  CTCGAG               C^TCGAG
XmnI         1288  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AocI          297  CCTAAGG              CC^T[ACGT]AGG
             1834  CCTGAGG             
BamHI         468  GGATCC               G^GATCC
             1961  GGATCC              
BbsI          201  GAAGAC               GAAGAC(2/6)
              807  GTCTTC              
BcnI         1965  CCGGG                CC^[GC]GG
             2019  CCGGG               
BpuAI         201  GAAGAC               GAAGAC(2/6)
              807  GTCTTC              
BsaBI         463  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              994  GATTACGATC          
BsiEI         425  CGACCG               CG[GA][CT]^CG
              999  CGATCG              
BsrFI          97  ACCGGC               [GA]^CCGG[CT]
             1378  ACCGGC              
BstYI         468  GGATCC               [GA]^GATC[CT]
             1961  GGATCC              
Bsu36I        297  CCTAAGG              CC^T[ACGT]AGG
             1834  CCTGAGG             
Cfr10I         97  ACCGGC               [GA]^CCGG[CT]
             1378  ACCGGC              
DsaI          342  CCACGG               C^C[GA][CT]GG
             1187  CCACGG              
EarI          444  GAAGAG               CTCTTC(1/4)
             1794  GAAGAG              
Eco81I        297  CCTAAGG              CC^T[ACGT]AGG
             1834  CCTGAGG             
EcoT22I       171  ATGCAT               ATGCA^T
             1389  ATGCAT              
Ksp632I       444  GAAGAG               CTCTTC(1/4)
             1794  GAAGAG              
MamI          463  GATCTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              994  GATTACGATC          
MflI          468  GGATCC               [GA]^GATC[CT]
             1961  GGATCC              
MseI          911  TTAA                 T^TAA
             2098  TTAA                
NciI         1965  CCGGG                CC^[GC]GG
             2019  CCGGG               
NsiI          171  ATGCAT               ATGCA^T
             1389  ATGCAT              
PpuMI         771  GGGACCC              [GA]G^G[AT]CC[CT]
             1991  AGGACCT             
TaqI          667  TCGA                 T^CGA
             1002  TCGA                
Tru9I         911  TTAA                 T^TAA
             2098  TTAA                
TthHB8I       667  TCGA                 T^CGA
             1002  TCGA                
XbaI          709  TCTAGA               T^CTAGA
             1575  TCTAGA              
XhoII         468  GGATCC               [GA]^GATC[CT]
             1961  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BanI        BanII       BbvCI       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        TseI        Tsp45I      TspRI       
XcmI        
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