Restriction map for pF1KSDA1253
 (Linear) (Six-base) MAPSORT of: KIAA1253.seq  Check: 5507  from: 1  to: 1359



KIAA1253.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     463    1359 
   Size:        463     896
 
AccI GT'mk_AC

Cuts at:      0     653     806     870    1359 
   Size:        653     153      64     489

  Fragments arranged by size: 

                 653     489     153      64
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1359 
   Size:        776     583
 
ApaLI G'TGCA_C

Cuts at:      0     361    1359 
   Size:        361     998
 
ApoI r'AATT_y

Cuts at:      0     385    1359 
   Size:        385     974
 
AseI AT'TA_AT

Cuts at:      0    1057    1359 
   Size:       1057     302
 
AvaI C'yCGr_G

Cuts at:      0     570    1359 
   Size:        570     789
 
BanI G'GyrC_C

Cuts at:      0     475    1326    1359 
   Size:        475     851      33

  Fragments arranged by size: 

                 851     475      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     782    1359 
   Size:        782     577
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      32    1359 
   Size:         32    1327
 
BclI T'GATC_A

Cuts at:      0     323    1359 
   Size:        323    1036
 
BglII A'GATC_T

Cuts at:      0     772    1359 
   Size:        772     587
 
Bme1580I G_kGCm'C

Cuts at:      0      63     365    1359 
   Size:         63     302     994

  Fragments arranged by size: 

                 994     302      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1114    1359 
   Size:       1114     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    1359 
   Size:        929     430
 
BsaBI GATnn'nnATC

Cuts at:      0    1297    1359 
   Size:       1297      62
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     137     167    1359 
   Size:        137      30    1192

  Fragments arranged by size: 

                1192     137      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     703    1359 
   Size:        703     656
 
BsiHKAI G_wGCw'C

Cuts at:      0      25     365    1359 
   Size:         25     340     994

  Fragments arranged by size: 

                 994     340      25
 
BsmI GAATG_Cn'

Cuts at:      0     418    1359 
   Size:        418     941
 
Bsp1286I G_dGCh'C

Cuts at:      0      25      63     365    1359 
   Size:         25      38     302     994

  Fragments arranged by size: 

                 994     302      38      25
 
BspHI T'CATG_A

Cuts at:      0    1199    1359 
   Size:       1199     160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     463    1359 
   Size:        463     896
 
BssSI C'ACGA_G

Cuts at:      0    1238    1321    1359 
   Size:       1238      83      38

  Fragments arranged by size: 

                1238      83      38
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     400    1359 
   Size:        400     959
 
BstYI r'GATC_y

Cuts at:      0     772    1359 
   Size:        772     587
 
BtgI C'CryG_G

Cuts at:      0      26    1359 
   Size:         26    1333
 
BtsI GCAGTG_nn'

Cuts at:      0     348     365    1359 
   Size:        348      17     994

  Fragments arranged by size: 

                 994     348      17
 
DraI TTT'AAA

Cuts at:      0     384    1359 
   Size:        384     975
 
EarI CTCTTCn'nnn_

Cuts at:      0     328    1287    1359 
   Size:        328     959      72

  Fragments arranged by size: 

                 959     328      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1114    1359 
   Size:        776     338     245

  Fragments arranged by size: 

                 776     338     245
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     950     982    1236    1268    1359 
   Size:        950      32     254      32      91

  Fragments arranged by size: 

                 950     254      91      32      32
 
HincII GTy'rAC

Cuts at:      0     705    1359 
   Size:        705     654
 
MfeI C'AATT_G

Cuts at:      0     398    1359 
   Size:        398     961
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     123    1270    1359 
   Size:        123    1147      89

  Fragments arranged by size: 

                1147     123      89
 
MslI CAynn'nnrTG

Cuts at:      0      48     528    1359 
   Size:         48     480     831

  Fragments arranged by size: 

                 831     480      48
 
MspA1I CmG'CkG

Cuts at:      0    1264    1359 
   Size:       1264      95
 
NcoI C'CATG_G

Cuts at:      0      26    1359 
   Size:         26    1333
 
NspI r_CATG'y

Cuts at:      0     711     943    1359 
   Size:        711     232     416

  Fragments arranged by size: 

                 711     416     232
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     775    1359 
   Size:        775     584
 
PfoI T'CCnGG_A

Cuts at:      0     204    1359 
   Size:        204    1155
 
PshAI GACnn'nnGTC

Cuts at:      0    1264    1359 
   Size:       1264      95
 
PstI C_TGCA'G

Cuts at:      0     360     396    1359 
   Size:        360      36     963

  Fragments arranged by size: 

                 963     360      36
 
PvuII CAG'CTG

Cuts at:      0    1264    1359 
   Size:       1264      95
 
SfcI C'TryA_G

Cuts at:      0     356     392     602    1121    1359 
   Size:        356      36     210     519     238

  Fragments arranged by size: 

                 519     356     238     210      36
 
SmlI C'TyrA_G

Cuts at:      0     570     944    1359 
   Size:        570     374     415

  Fragments arranged by size: 

                 570     415     374
 
SpeI A'CTAG_T

Cuts at:      0     501    1359 
   Size:        501     858
 
SphI G_CATG'C

Cuts at:      0     943    1359 
   Size:        943     416
 
StyI C'CwwG_G

Cuts at:      0      26    1359 
   Size:         26    1333
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1260    1359 
   Size:       1260      99
 
Tth111I GACn'n_nGTC

Cuts at:      0     825    1359 
   Size:        825     534
 
XhoI C'TCGA_G

Cuts at:      0     570    1359 
   Size:        570     789
 

 Enzymes that do cut:

     AarI     AccI     AcuI    ApaLI     ApoI     AseI     AvaI     BanI
     BbsI    BciVI     BclI    BglII Bme1580I     BpmI    BpuEI    BsaBI
    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BssSI
   BstAPI    BstYI     BtgI     BtsI     DraI     EarI  Eco57MI    Hin4I
   HincII     MfeI     MmeI     MslI   MspA1I     NcoI     NspI    PflMI
     PfoI    PshAI     PstI    PvuII     SfcI     SmlI     SpeI     SphI
     StyI    TaqII  Tth111I     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     StuI
     SwaI     TatI     TstI     XbaI     XcmI     XmaI     XmnI     ZraI
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