Restriction map for pF1KSDA1214
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       BbeI        
BbrPI       BfrI        BglII       BlpI        Bpu1102I    
BsaBI       BseRI       BsiWI       BsmI        BspDI       
BspHI       BspLU11I    BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraII       DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        Ksp632I     MamI        
MfeI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tsp509I     Van91I      XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1051  GACGTC               GACGT^C
AflIII        401  ACGCGT               A^C[GA][CT]GT
AlwNI         686  CAGAGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       399  GAACGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         235  GAGCAC               G[AT]GC[AT]^C
AspI          739  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          163  CCCGGG               C^[CT]CG[GA]G
AvrII         849  CCTAGG               C^CTAGG
BanI          940  GGTGCC               G^G[CT][GA]CC
BbvCI         419  CCTCAGC              CCTCAGC(-5/-2)
BcgI          600  GCAGAAATATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1023  GGATAC               GTATCC(6/5)
BclI         1115  TGATCA               T^GATCA
BlnI          849  CCTAGG               C^CTAGG
BpmI          649  CTCCAG               CTGGAG(16/14)
BsaAI         378  CACGTA               [CT]AC^GT[GA]
BsaI          460  GAGACC               GGTCTC(1/5)
BsiEI         291  CGACCG               CG[GA][CT]^CG
BsiHKAI       235  GAGCAC               G[AT]GC[AT]^C
BsmBI        1049  GAGACG               CGTCTC(1/5)
BsmFI         818  GTCCC                GGGAC(10/14)
BsoBI         163  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      572  TGTACA               T^GTACA
BspMI        1011  GCAGGT               ACCTGC(4/8)
BsrFI         761  GCCGGC               [GA]^CCGG[CT]
BsrGI         572  TGTACA               T^GTACA
Cfr10I        761  GCCGGC               [GA]^CCGG[CT]
DrdI          982  GACCCTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         1115  TGATCA               T^GATCA
HaeII         224  GGCGCT               [GA]GCGC^[CT]
HgaI          253  GACGC                GACGC(5/10)
HincII        787  GTTGAC               GT[CT]^[GA]AC
HindII        787  GTTGAC               GT[CT]^[GA]AC
MluI          401  ACGCGT               A^CGCGT
MseI          842  TTAA                 T^TAA
NaeI          761  GCCGGC               GCC^GGC
NgoMIV        761  GCCGGC               G^CCGGC
PflFI         739  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          688  GAGTC                GAGTC(4/5)
PvuII        1085  CAGCTG               CAG^CTG
SfiI          276  GGCCAGCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          163  CCCGGG               CCC^GGG
SspBI         572  TGTACA               T^GTACA
TaqI          899  TCGA                 T^CGA
Tru9I         842  TTAA                 T^TAA
Tth111I       739  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       899  TCGA                 T^CGA
XbaI          801  TCTAGA               T^CTAGA
XmaCI         163  CCCGGG               C^CCGGG
XmaI          163  CCCGGG               C^CCGGG
XmnI          399  GAACGCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          253  GACGCC               G[GA]^CG[CT]C
             1051  GACGTC              
BalI          275  TGGCCA               TGG^CCA
              500  TGGCCA              
BamHI         749  GGATCC               G^GATCC
              854  GGATCC              
BbsI          210  GAAGAC               GAAGAC(2/6)
              490  GAAGAC              
BfaI          802  CTAG                 C^TAG
              850  CTAG                
BpuAI         210  GAAGAC               GAAGAC(2/6)
              490  GAAGAC              
BsaHI         253  GACGCC               G[GA]^CG[CT]C
             1051  GACGTC              
BsrDI           4  GCAATG               GCAATG(2/0)
             1123  GCAATG              
BsrI          347  ACTGG                ACTGG(1/-1)
              615  ACTGG               
DdeI          420  CTCAG                C^T[ACGT]AG
              515  CTGAG               
EcoT14I       365  CCAAGG               C^C[AT][AT]GG
              849  CCTAGG              
Hin1I         253  GACGCC               G[GA]^CG[CT]C
             1051  GACGTC              
KspI          258  CCGCGG               CCGC^GG
              336  CCGCGG              
MaeI          802  CTAG                 C^TAG
              850  CTAG                
MluNI         275  TGGCCA               TGG^CCA
              500  TGGCCA              
MscI          275  TGGCCA               TGG^CCA
              500  TGGCCA              
PstI           27  CTGCAG               CTGCA^G
              409  CTGCAG              
SacII         258  CCGCGG               CCGC^GG
              336  CCGCGG              
SfcI           27  CTGCAG               C^T[GA][CT]AG
              409  CTGCAG              
StyI          365  CCAAGG               C^C[AT][AT]GG
              849  CCTAGG              
TfiI          510  GATTC                G^A[AT]TC
             1099  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AccIII      AciI        AfaI        AluI        
AlwI        AvaII       BanII       BbvI        BcnI        
BglI        BmyI        BsaJI       BsaWI       BseAI       
BsgI        BsiYI       BslI        BsmAI       Bsp1286I    
BspEI       BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DpnII       
DsaI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MroI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        Tsp45I      TspRI       XhoII       
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