Restriction map for pF1KSDA1195
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
AviII       AvrII       BamHI       BbeI        BbsI        
BbvCI       BclI        BcnI        BfrI        BglI        
BlnI        BpuAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BspEI       
BsrBI       BsrFI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        540  GGTACC               G^GTACC
AhdI         1492  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        934  GTGCAC               G^TGCAC
AlwNI         680  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         934  GTGCAC               G^TGCAC
Asp718I       540  GGTACC               G^GTACC
AspEI        1492  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2072  CCCGAG               C^[CT]CG[GA]G
BbrPI        1366  CACGTG               CAC^GTG
BcgI          825  GCAGGACGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1134  GGATAC               GTATCC(6/5)
BlpI         1656  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1656  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1366  CACGTG               [CT]AC^GT[GA]
BsaI          352  GAGACC               GGTCTC(1/5)
BsgI         1468  CTGCAC               GTGCAG(16/14)
BsmBI        2121  CGTCTC               CGTCTC(1/5)
BsoBI        2072  CCCGAG               C^[CT]CG[GA]G
BspDI        1526  ATCGAT               AT^CGAT
BssSI        2108  CACGAG               CACGAG(-5/-1)
BstAPI        679  GCAGCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1656  GCTGAGC              GC^T[ACGT]AGC
CfoI          785  GCGC                 GCG^C
ClaI         1526  ATCGAT               AT^CGAT
Eam1105I     1492  GACATTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1710  GAATTC               G^AATTC
EcoRV         529  GATATC               GAT^ATC
HaeII         784  GGCGCT               [GA]GCGC^[CT]
HapII        1639  CCGG                 C^CGG
HgaI         1769  GCGTC                GACGC(5/10)
HhaI          785  GCGC                 GCG^C
HinP1I        785  GCGC                 G^CGC
HpaII        1639  CCGG                 C^CGG
KpnI          540  GGTACC               GGTAC^C
MspI         1639  CCGG                 C^CGG
NheI          606  GCTAGC               G^CTAGC
PmaCI        1366  CACGTG               CAC^GTG
PmlI         1366  CACGTG               CAC^GTG
PvuII         899  CAGCTG               CAG^CTG
SexAI         632  ACCTGGT              A^CC[AT]GGT
SmlI         1618  CTCAAG               C^T[CT][GA]AG
SspI          658  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        140  ACATGT               A^C[GA][CT]GT
              923  ACATGT              
AspI          631  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1765  GACAGCGTC           
BglII        1179  AGATCT               A^GATCT
             1913  AGATCT              
Bsp1407I      979  TGTACA               T^GTACA
             1552  TGTACA              
BspHI         333  TCATGA               T^CATGA
              344  TCATGA              
BspLU11I      140  ACATGT               A^CATGT
              923  ACATGT              
BsrDI        1749  CATTGC               GCAATG(2/0)
             1857  GCAATG              
BsrGI         979  TGTACA               T^GTACA
             1552  TGTACA              
DraII         404  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1163  AGGACCT             
EarI          409  CTCTTC               CTCTTC(1/4)
             1933  CTCTTC              
EcoO109I      404  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1163  AGGACCT             
HphI          484  GGTGA                GGTGA(8/7)
             1970  TCACC               
Ksp632I       409  CTCTTC               CTCTTC(1/4)
             1933  CTCTTC              
MslI           72  CATTCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1971  CACCTCCATG          
PflFI         631  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1765  GACAGCGTC           
PpuMI         404  AGGTCCT              [GA]G^G[AT]CC[CT]
             1163  AGGACCT             
PstI          253  CTGCAG               CTGCA^G
              391  CTGCAG              
RcaI          333  TCATGA               T^CATGA
              344  TCATGA              
SbfI          252  CCTGCAGG             CCTGCA^GG
              390  CCTGCAGG            
Sse8387I      252  CCTGCAGG             CCTGCA^GG
              390  CCTGCAGG            
SspBI         979  TGTACA               T^GTACA
             1552  TGTACA              
TaqI         1083  TCGA                 T^CGA
             1527  TCGA                
Tsp45I       1390  GTGAC                ^GT[GC]AC
             1969  GTCAC               
Tth111I       631  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1765  GACAGCGTC           
TthHB8I      1083  TCGA                 T^CGA
             1527  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MspA1I      MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       Van91I      XcmI        XhoII       
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