Restriction map for pF1KSDA1195
 (Linear) (Six-base) MAPSORT of: KIAA1195.seq  Check: 1840  from: 1  to: 2127



KIAA1195.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     125     246     260    2127 
   Size:        125     121      14    1867

  Fragments arranged by size: 

                1867     125     121      14
 
Acc65I G'GTAC_C

Cuts at:      0     540    2127 
   Size:        540    1587
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396    1124    1431    2011    2127 
   Size:        396     728     307     580     116

  Fragments arranged by size: 

                 728     580     396     307     116
 
AflIII A'CryG_T

Cuts at:      0     140     923    2127 
   Size:        140     783    1204

  Fragments arranged by size: 

                1204     783     140
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1497    2127 
   Size:       1497     630
 
AlwNI CAG_nnn'CTG

Cuts at:      0     685    2127 
   Size:        685    1442
 
ApaLI G'TGCA_C

Cuts at:      0     934    2127 
   Size:        934    1193
 
ApoI r'AATT_y

Cuts at:      0     696    1441    1710    2127 
   Size:        696     745     269     417

  Fragments arranged by size: 

                 745     696     417     269
 
AvaI C'yCGr_G

Cuts at:      0    2072    2127 
   Size:       2072      55
 
BanI G'GyrC_C

Cuts at:      0      29     127     540    1888    2127 
   Size:         29      98     413    1348     239

  Fragments arranged by size: 

                1348     413     239      98      29
 
BanII G_rGCy'C

Cuts at:      0    1083    1376    1619    2127 
   Size:       1083     293     243     508

  Fragments arranged by size: 

                1083     508     293     243
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     814     848    2127 
   Size:        814      34    1279

  Fragments arranged by size: 

                1279     814      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1128    2127 
   Size:       1128     999
 
BglII A'GATC_T

Cuts at:      0    1179    1913    2127 
   Size:       1179     734     214

  Fragments arranged by size: 

                1179     734     214
 
BlpI GC'TnA_GC

Cuts at:      0    1657    2127 
   Size:       1657     470
 
Bme1580I G_kGCm'C

Cuts at:      0      32     265     938    2127 
   Size:         32     233     673    1189

  Fragments arranged by size: 

                1189     673     233      32
 
BmgBI CAC'GTC

Cuts at:      0    1233    2127 
   Size:       1233     894
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     353    2127 
   Size:        353    1774
 
BmtI G_CTAG'C

Cuts at:      0     610    2127 
   Size:        610    1517
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1036    1183    1634    1753    1917    2127 
   Size:       1036     147     451     119     164     210

  Fragments arranged by size: 

                1036     451     210     164     147     119
 
Bpu10I CC'TnA_GC

Cuts at:      0    1022    2127 
   Size:       1022    1105
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1603    2127 
   Size:       1603     524
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     346    2127 
   Size:        346    1781
 
BsaAI yAC'GTr

Cuts at:      0    1368    2127 
   Size:       1368     759
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     500     530    2127 
   Size:        500      30    1597

  Fragments arranged by size: 

                1597     500      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     379     478     524    2127 
   Size:        157     222      99      46    1603

  Fragments arranged by size: 

                1603     222     157      99      46
 
BseYI C'CCAG_C

Cuts at:      0    1753    1781    1952    2127 
   Size:       1753      28     171     175

  Fragments arranged by size: 

                1753     175     171      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1453    2127 
   Size:       1453     674
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     677     938    1083    1376    1619    1988    2021 
   Size:        161     516     261     145     293     243     369      33

Cuts at:   2021    2127 
   Size:        106

  Fragments arranged by size: 

                 516     369     293     261     243     161     145     106
                  33
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2127 
   Size:       2127
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     161     265     677     938    1083    1376    1619 
   Size:         32     129     104     412     261     145     293     243

Cuts at:   1619    1988    2021    2127 
   Size:        369      33     106

  Fragments arranged by size: 

                 412     369     293     261     243     145     129     106
                 104      33      32
 
BspHI T'CATG_A

Cuts at:      0     333     344    2127 
   Size:        333      11    1783

  Fragments arranged by size: 

                1783     333      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     174     246     260    1175    2127 
   Size:        125      49      72      14     915     952

  Fragments arranged by size: 

                 952     915     125      72      49      14
 
BsrDI GCAATG_nn'

Cuts at:      0    1748    1864    2127 
   Size:       1748     116     263

  Fragments arranged by size: 

                1748     263     116
 
BsrGI T'GTAC_A

Cuts at:      0     979    1552    2127 
   Size:        979     573     575

  Fragments arranged by size: 

                 979     575     573
 
BssSI C'ACGA_G

Cuts at:      0    2108    2127 
   Size:       2108      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     685    2127 
   Size:        685    1442
 
BstYI r'GATC_y

Cuts at:      0      16     415    1179    1913    2127 
   Size:         16     399     764     734     214

  Fragments arranged by size: 

                 764     734     399     214      16
 
BtgI C'CryG_G

Cuts at:      0     287     842    1881    2127 
   Size:        287     555    1039     246

  Fragments arranged by size: 

                1039     555     287     246
 
BtsI GCAGTG_nn'

Cuts at:      0      63     521    1136    2127 
   Size:         63     458     615     991

  Fragments arranged by size: 

                 991     615     458      63
 
ClaI AT'CG_AT

Cuts at:      0    1527    2127 
   Size:       1527     600
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      22      57     840     875    2127 
   Size:         22      35     783      35    1252

  Fragments arranged by size: 

                1252     783      35      35      22
 
EaeI y'GGCC_r

Cuts at:      0     242     304     645     759    2127 
   Size:        242      62     341     114    1368

  Fragments arranged by size: 

                1368     341     242     114      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     415    1939    2127 
   Size:        415    1524     188

  Fragments arranged by size: 

                1524     415     188
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     960    2127 
   Size:        960    1167
 
EcoICRI GAG'CTC

Cuts at:      0    1081    1374    1617    2127 
   Size:       1081     293     243     510

  Fragments arranged by size: 

                1081     510     293     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     396    1036    1124    1183    1431    1634    1753    1917 
   Size:        396     640      88      59     248     203     119     164

Cuts at:   1917    2011    2127 
   Size:         94     116

  Fragments arranged by size: 

                 640     396     248     203     164     119     116      94
                  88      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0     405    1164    2127 
   Size:        405     759     963

  Fragments arranged by size: 

                 963     759     405
 
EcoRI G'AATT_C

Cuts at:      0    1710    2127 
   Size:       1710     417
 
EcoRV GAT'ATC

Cuts at:      0     531    2127 
   Size:        531    1596
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     426    2127 
   Size:        426    1701
 
HaeII r_GCGC'y

Cuts at:      0     788    2127 
   Size:        788    1339
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     515     547    1483    1515    1520    1552    1832    1864 
   Size:        515      32     936      32       5      32     280      32

Cuts at:   1864    2127 
   Size:        263

  Fragments arranged by size: 

                 936     515     280     263      32      32      32      32
                   5
 
KpnI G_GTAC'C

Cuts at:      0     544    2127 
   Size:        544    1583
 
MscI TGG'CCA

Cuts at:      0     244     306     647     761    2127 
   Size:        244      62     341     114    1366

  Fragments arranged by size: 

                1366     341     244     114      62
 
MslI CAynn'nnrTG

Cuts at:      0      76    1975    2127 
   Size:         76    1899     152

  Fragments arranged by size: 

                1899     152      76
 
MspA1I CmG'CkG

Cuts at:      0     302     901    1345    2127 
   Size:        302     599     444     782

  Fragments arranged by size: 

                 782     599     444     302
 
NcoI C'CATG_G

Cuts at:      0     287     842    1881    2127 
   Size:        287     555    1039     246

  Fragments arranged by size: 

                1039     555     287     246
 
NheI G'CTAG_C

Cuts at:      0     606    2127 
   Size:        606    1521
 
NspI r_CATG'y

Cuts at:      0     144     927    2052    2127 
   Size:        144     783    1125      75

  Fragments arranged by size: 

                1125     783     144      75
 
PasI CC'CwG_GG

Cuts at:      0    1354    1582    2127 
   Size:       1354     228     545

  Fragments arranged by size: 

                1354     545     228
 
PciI A'CATG_T

Cuts at:      0     140     923    2127 
   Size:        140     783    1204

  Fragments arranged by size: 

                1204     783     140
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240     293     768    1686    2127 
   Size:        240      53     475     918     441

  Fragments arranged by size: 

                 918     475     441     240      53
 
PfoI T'CCnGG_A

Cuts at:      0    1894    2127 
   Size:       1894     233
 
PmlI CAC'GTG

Cuts at:      0    1368    2127 
   Size:       1368     759
 
PpuMI rG'GwC_Cy

Cuts at:      0     405    1164    2127 
   Size:        405     759     963

  Fragments arranged by size: 

                 963     759     405
 
PstI C_TGCA'G

Cuts at:      0     257     395    2127 
   Size:        257     138    1732

  Fragments arranged by size: 

                1732     257     138
 
PvuII CAG'CTG

Cuts at:      0     901    2127 
   Size:        901    1226
 
SacI G_AGCT'C

Cuts at:      0    1083    1376    1619    2127 
   Size:       1083     293     243     508

  Fragments arranged by size: 

                1083     508     293     243
 
SbfI CC_TGCA'GG

Cuts at:      0     257     395    2127 
   Size:        257     138    1732

  Fragments arranged by size: 

                1732     257     138
 
SexAI A'CCwGG_T

Cuts at:      0     632    2127 
   Size:        632    1495
 
SfcI C'TryA_G

Cuts at:      0     253     391    1605    2127 
   Size:        253     138    1214     522

  Fragments arranged by size: 

                1214     522     253     138
 
SmlI C'TyrA_G

Cuts at:      0    1618    2127 
   Size:       1618     509
 
SspI AAT'ATT

Cuts at:      0     660    2127 
   Size:        660    1467
 
StyI C'CwwG_G

Cuts at:      0     287     842     865     986    1881    2127 
   Size:        287     555      23     121     895     246

  Fragments arranged by size: 

                 895     555     287     246     121      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     508     999    2127 
   Size:        508     491    1128

  Fragments arranged by size: 

                1128     508     491
 
TatI w'GTAC_w

Cuts at:      0     979    1552    1745    2127 
   Size:        979     573     193     382

  Fragments arranged by size: 

                 979     573     382     193
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     500     532    1352    1384    1803    1835    1978    2010 
   Size:        500      32     820      32     419      32     143      32

Cuts at:   2010    2127 
   Size:        117

  Fragments arranged by size: 

                 820     500     419     143     117      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     634    1768    2127 
   Size:        634    1134     359

  Fragments arranged by size: 

                1134     634     359
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     121     624    1728    2127 
   Size:        121     503    1104     399

  Fragments arranged by size: 

                1104     503     399     121
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AhdI    AlwNI    ApaLI     ApoI
     AvaI     BanI    BanII     BcgI    BciVI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstYI     BtgI     BtsI
     ClaI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    HaeII    Hin4I     KpnI     MscI     MslI
   MspA1I     NcoI     NheI     NspI     PasI     PciI    PflMI     PfoI
     PmlI    PpuMI     PstI    PvuII     SacI     SbfI    SexAI     SfcI
     SmlI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BclI    BfrBI     BglI     BplI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrFI
   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     DraI
   DraIII     DrdI     EagI    EcoNI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     XbaI
     XhoI     XmaI     XmnI     ZraI
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