Restriction map for pF1KSDA1165
 (Linear) (Six-base) MAPSORT of: KIAA1165.seq  Check: 5604  from: 1  to: 726



KIAA1165.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     185     357     624     726 
   Size:        113      72     172     267     102

  Fragments arranged by size: 

                 267     172     113     102      72
 
AflIII A'CryG_T

Cuts at:      0     674     726 
   Size:        674      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     433     726 
   Size:        433     293
 
ApoI r'AATT_y

Cuts at:      0     338     726 
   Size:        338     388
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     197     230     726 
   Size:        197      33     496

  Fragments arranged by size: 

                 496     197      33
 
BamHI G'GATC_C

Cuts at:      0     542     726 
   Size:        542     184
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     726 
   Size:        119     607
 
BclI T'GATC_A

Cuts at:      0     533     726 
   Size:        533     193
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      32     726 
   Size:         32     694
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      75     726 
   Size:         75     651
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     337     367     726 
   Size:        337      30     359

  Fragments arranged by size: 

                 359     337      30
 
BseYI C'CCAG_C

Cuts at:      0      99     726 
   Size:         99     627
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     726 
   Size:        136     590
 
BsrDI GCAATG_nn'

Cuts at:      0     311     726 
   Size:        311     415
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     114     726 
   Size:        114     612
 
BstYI r'GATC_y

Cuts at:      0     542     726 
   Size:        542     184
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     159     269     344     726 
   Size:         44     115     110      75     382

  Fragments arranged by size: 

                 382     115     110      75      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     185     357     624     726 
   Size:        113      72     172     267     102

  Fragments arranged by size: 

                 267     172     113     102      72
 
EcoRI G'AATT_C

Cuts at:      0     338     726 
   Size:        338     388
 
HaeII r_GCGC'y

Cuts at:      0      30     726 
   Size:         30     696
 
MslI CAynn'nnrTG

Cuts at:      0     415     726 
   Size:        415     311
 
MspA1I CmG'CkG

Cuts at:      0     313     694     726 
   Size:        313     381      32

  Fragments arranged by size: 

                 381     313      32
 
NspI r_CATG'y

Cuts at:      0     678     726 
   Size:        678      48
 
PciI A'CATG_T

Cuts at:      0     674     726 
   Size:        674      52
 
PstI C_TGCA'G

Cuts at:      0     318     726 
   Size:        318     408
 
PvuII CAG'CTG

Cuts at:      0     313     694     726 
   Size:        313     381      32

  Fragments arranged by size: 

                 381     313      32
 
SexAI A'CCwGG_T

Cuts at:      0      32     726 
   Size:         32     694
 
SfcI C'TryA_G

Cuts at:      0      74     246     314     726 
   Size:         74     172      68     412

  Fragments arranged by size: 

                 412     172      74      68
 
TatI w'GTAC_w

Cuts at:      0     618     726 
   Size:        618     108
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     337     369     726 
   Size:        337      32     357

  Fragments arranged by size: 

                 357     337      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      19     726 
   Size:         19     707
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     ApoI     BaeI    BamHI     BbsI     BclI
     BmrI     BplI    BsaXI    BseYI     BsgI    BsrDI   BstAPI    BstYI
     EarI  Eco57MI    EcoRI    HaeII     MslI   MspA1I     NspI     PciI
     PstI    PvuII    SexAI     SfcI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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