Restriction map for pF1KSDA1155
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# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AluI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   56 enzymes) #
###################################

AciI          174  CCGC                 CCGC(-3/-1)
AflIII        101  ACATGT               A^C[GA][CT]GT
AhdI          275  GACTGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          238  CCTCAGG              CC^T[ACGT]AGG
AspEI         275  GACTGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI           7  GGATCC               G^GATCC
BbsI          202  GAAGAC               GAAGAC(2/6)
BbvI          176  GCTGC                GCAGC(8/12)
BmyI           67  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         202  GAAGAC               GAAGAC(2/6)
BsaI          230  GAGACC               GGTCTC(1/5)
BseRI         142  GAGGAG               GAGGAG(10/8)
BsiYI         234  CCTGCCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          234  CCTGCCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         230  GAGAC                GTCTC(1/5)
BsmFI         249  GGGAC                GGGAC(10/14)
Bsp1286I       67  GGGCAC               G[AGT]GC[ACT]^C
BspHI         294  TCATGA               T^CATGA
BspLU11I      101  ACATGT               A^CATGT
BspMI         233  ACCTGC               ACCTGC(4/8)
BsrI          212  ACTGG                ACTGG(1/-1)
BssSI         227  CACGAG               CACGAG(-5/-1)
BstYI           7  GGATCC               [GA]^GATC[CT]
Bsu36I        238  CCTCAGG              CC^T[ACGT]AGG
DdeI          239  CTCAG                C^T[ACGT]AG
DpnII           8  GATC                 ^GATC
DraIII         29  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          245  CCATGG               C^C[GA][CT]GG
Eam1105I      275  GACTGTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          118  GAAGAG               CTCTTC(1/4)
Eco81I        238  CCTCAGG              CC^T[ACGT]AGG
EcoNI         234  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        176  GCTGC                GC^[ACGT]GC
HaeIII        243  GGCC                 GG^CC
HinfI         298  GATTC                G^A[ACGT]TC
HphI           28  TCACC                GGTGA(8/7)
ItaI          176  GCTGC                GC^[ACGT]GC
Ksp632I       118  GAAGAG               CTCTTC(1/4)
MaeIII        323  GTAAC                ^GT[ACGT]AC
MboI            8  GATC                 ^GATC
MflI            7  GGATCC               [GA]^GATC[CT]
MslI          102  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        174  CCGCTG               C[AC]G^C[GT]G
NcoI          245  CCATGG               C^CATGG
NdeII           8  GATC                 ^GATC
NlaIV           7  GGATCC               GG[ACGT]^[ACGT]CC
NspI          101  ACATGT               [GA]CATG^[CT]
PstI          151  CTGCAG               CTGCA^G
RcaI          294  TCATGA               T^CATGA
Sau3AI          8  GATC                 ^GATC
SfcI          151  CTGCAG               C^T[GA][CT]AG
TfiI          298  GATTC                G^A[AT]TC
TseI          176  GCTGC                G^C[AT]GC
TspRI         285  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI           54  CCAGGGGTTAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII           7  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI           99  GTAC                 GT^AC
              366  GTAC                
AlwI            8  GATCC                GGATC(4/5)
                7  GGATC               
AvaII          51  GGACC                G^G[AT]CC
              171  GGACC               
BssKI          54  CCAGG                ^CC[ACGT]GG
              355  CCAGG               
BstF5I        107  GGATG                GGATG(2/0)
              195  GGATG               
BstNI          54  CCAGG                CC^[AT]GG
              355  CCAGG               
Cfr13I         51  GGACC                G^G[ACGT]CC
              171  GGACC               
EcoRII         54  CCAGG                ^CC[AT]GG
              355  CCAGG               
EcoT14I       245  CCATGG               C^C[AT][AT]GG
              341  CCAAGG              
FokI          107  GGATG                GGATG(9/13)
              195  GGATG               
MseI           61  TTAA                 T^TAA
              268  TTAA                
MvaI           54  CCAGG                CC^[AT]GG
              355  CCAGG               
RsaI           99  GTAC                 GT^AC
              366  GTAC                
Sau96I         51  GGACC                G^G[ACGT]CC
              171  GGACC               
ScrFI          54  CCAGG                CC^[ACGT]GG
              355  CCAGG               
SfaNI         189  GATGC                GCATC(5/9)
              337  GATGC               
StyI          245  CCATGG               C^C[AT][AT]GG
              341  CCAAGG              
Tru9I          61  TTAA                 T^TAA
              268  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BsaJI       MboII       MnlI        NlaIII      
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