Restriction map for
pF1KSDA1152
(Circular) (Six-base) MAPSORT of: pF1KSDA1152.seq Check: 2166 from: 1 to: 4192
pF1KSDA1152 4192 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 495 1053 1228 495
Size: 558 175 3459
Fragments arranged by size:
3459 558 175
Acc65I G'GTAC_C
Cuts at: 1031 1206 1031
Size: 175 4017
AclI AA'CG_TT
Cuts at: 4032 4032
Size: 4192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 586 971 2015 2447 3196 3464 538
Size: 48 385 1044 432 749 268 1266
Fragments arranged by size:
1266 1044 749 432 385 268 48
AflIII A'CryG_T
Cuts at: 776 1542 2648 3625 776
Size: 766 1106 977 1343
Fragments arranged by size:
1343 1106 977 766
AgeI A'CCGG_T
Cuts at: 2580 2580
Size: 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1089 1089
Size: 4192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 252 284 252
Size: 32 4160
AlwNI CAG_nnn'CTG
Cuts at: 564 627 1495 3064 564
Size: 63 868 1569 1692
Fragments arranged by size:
1692 1569 868 63
ApaLI G'TGCA_C
Cuts at: 2962 2962
Size: 4192
ApoI r'AATT_y
Cuts at: 982 1194 3545 3801 982
Size: 212 2351 256 1373
Fragments arranged by size:
2351 1373 256 212
AseI AT'TA_AT
Cuts at: 20 20
Size: 4192
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4192
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4192
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4192
BanI G'GyrC_C
Cuts at: 1031 1206 1620 1868 1903 1031
Size: 175 414 248 35 3320
Fragments arranged by size:
3320 414 248 175 35
BanII G_rGCy'C
Cuts at: 220 766 827 936 1204 2234 220
Size: 546 61 109 268 1030 2178
Fragments arranged by size:
2178 1030 546 268 109 61
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3944
BbvCI CC'TCA_GC
Cuts at: 153 153
Size: 4192
BciVI GTATCCnnnnn_n'
Cuts at: 1182 2088 2851 4192 1182
Size: 906 763 1341 1182
Fragments arranged by size:
1341 1182 906 763
BclI T'GATC_A
Cuts at: 939 1710 939
Size: 771 3421
BglII A'GATC_T
Cuts at: 1705 3744 1705
Size: 2039 2153
BlpI GC'TnA_GC
Cuts at: 214 1302 214
Size: 1088 3104
Bme1580I G_kGCm'C
Cuts at: 1815 1908 2966 1815
Size: 93 1058 3041
Fragments arranged by size:
3041 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 830 1402 1483 1818 3964 830
Size: 572 81 335 2146 1058
Fragments arranged by size:
2146 1058 572 335 81
BmtI G_CTAG'C
Cuts at: 551 551
Size: 4192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 1079
Size: 4192
Bpu10I CC'TnA_GC
Cuts at: 153 706 153
Size: 553 3639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2739 3037 1366
Size: 879 494 298 2521
Fragments arranged by size:
2521 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3896 3896
Size: 4192
BsaAI yAC'GTr
Cuts at: 1182 1543 1182
Size: 361 3831
BsaBI GATnn'nnATC
Cuts at: 6 1704 3743 6
Size: 1698 2039 455
Fragments arranged by size:
2039 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2573 4069 1621
Size: 248 704 1496 1744
Fragments arranged by size:
1744 1496 704 248
BsaWI w'CCGG_w
Cuts at: 366 1379 1900 2580 2854 3001 3412 366
Size: 1013 521 680 274 147 411 1146
Fragments arranged by size:
1146 1013 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 878 908 878
Size: 30 4162
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 217
Size: 4192
BseYI C'CCAG_C
Cuts at: 417 1615 2373 2952 3448 417
Size: 1198 758 579 496 1161
Fragments arranged by size:
1198 1161 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2988 104
Size: 1628 46 1210 1308
Fragments arranged by size:
1628 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1982 2172 2966 1204
Size: 778 190 794 2430
Fragments arranged by size:
2430 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 220 766 827 936 1204 1815 1908 1982 2172
Size: 546 61 109 268 611 93 74 190
Cuts at: 2172 2234 2966 220
Size: 62 732 1446
Fragments arranged by size:
1446 732 611 546 268 190 109 93
74 62 61
BspEI T'CCGG_A
Cuts at: 3412 3412
Size: 4192
BspHI T'CATG_A
Cuts at: 3368 3368
Size: 4192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 307 590 1240 1454 1756 2137 307
Size: 283 650 214 302 381 2362
Fragments arranged by size:
2362 650 381 302 283 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4022 4187 50
Size: 2432 54 1486 165 55
Fragments arranged by size:
2432 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4192
BsrFI r'CCGG_y
Cuts at: 2188 2369 2580 2188
Size: 181 211 3800
Fragments arranged by size:
3800 211 181
BssSI C'ACGA_G
Cuts at: 2461 2821 2461
Size: 360 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4192
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4192
BstEII G'GTnAC_C
Cuts at: 369 369
Size: 4192
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3300 3386 3398
Size: 1214 467 23 335 246 1014 86 12
Cuts at: 3398 3744 1
Size: 346 449
Fragments arranged by size:
1214 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 669 125
Size: 544 3648
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3848
Fragments arranged by size:
3848 181 163
BtsI GCAGTG_nn'
Cuts at: 804 819 1433 804
Size: 15 614 3563
Fragments arranged by size:
3563 614 15
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4192
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2756 1896
Size: 860 3332
EaeI y'GGCC_r
Cuts at: 1015 1775 1949 2340 2367 3714 1015
Size: 760 174 391 27 1347 1493
Fragments arranged by size:
1493 1347 760 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4192
EarI CTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3982
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 528 2398 2710 2856 4002 528
Size: 1870 312 146 1146 718
Fragments arranged by size:
1870 1146 718 312 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 586 971 1079 2015 2447 3196 3464 538
Size: 48 385 108 936 432 749 268 1266
Fragments arranged by size:
1266 936 749 432 385 268 108 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1081 1081
Size: 4192
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4192
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4192
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4192
HaeII r_GCGC'y
Cuts at: 214 1624 1872 2896 3875 214
Size: 1410 248 1024 979 531
Fragments arranged by size:
1410 1024 979 531 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 154 168 186 763 795 878 893 910
Size: 18 14 18 577 32 83 15 17
Cuts at: 910 925 2032 2062 2064 2094 136
Size: 15 1107 30 2 30 2234
Fragments arranged by size:
2234 1107 577 83 32 30 30 18
18 17 15 15 14 2
HincII GTy'rAC
Cuts at: 496 1229 496
Size: 733 3459
HindIII A'AGCT_T
Cuts at: 184 184
Size: 4192
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3944
KpnI G_GTAC'C
Cuts at: 1035 1210 1035
Size: 175 4017
MluI A'CGCG_T
Cuts at: 3625 3625
Size: 4192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 238 2863 3047 238
Size: 2625 184 1383
Fragments arranged by size:
2625 1383 184
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4192
MslI CAynn'nnrTG
Cuts at: 2179 2306 2179
Size: 127 4065
MspA1I CmG'CkG
Cuts at: 635 1301 1615 1975 2990 3235 635
Size: 666 314 360 1015 245 1592
Fragments arranged by size:
1592 1015 666 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4192
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3944
NcoI C'CATG_G
Cuts at: 125 125
Size: 4192
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4192
NheI G'CTAG_C
Cuts at: 547 547
Size: 4192
NruI TCG'CGA
Cuts at: 2553 2553
Size: 4192
NspI r_CATG'y
Cuts at: 780 1243 1743 2652 780
Size: 463 500 909 2320
Fragments arranged by size:
2320 909 500 463
PciI A'CATG_T
Cuts at: 776 2648 776
Size: 1872 2320
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3331 3363 3331
Size: 32 4160
PstI C_TGCA'G
Cuts at: 601 1068 1237 1922 601
Size: 467 169 685 2871
Fragments arranged by size:
2871 685 467 169
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4192
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3832
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4192
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4192
SalI G'TCGA_C
Cuts at: 494 1227 494
Size: 733 3459
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3982
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4192
ScaI AGT'ACT
Cuts at: 3709 3709
Size: 4192
SfcI C'TryA_G
Cuts at: 33 306 597 1064 1233 1918 2913 3104 33
Size: 273 291 467 169 685 995 191 1121
Fragments arranged by size:
1121 995 685 467 291 273 191 169
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3944
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4192
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4192
SmlI C'TyrA_G
Cuts at: 1345 2260 2754 3016 1345
Size: 915 494 262 2521
Fragments arranged by size:
2521 915 494 262
SnaBI TAC'GTA
Cuts at: 1182 1182
Size: 4192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4192
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3692
SspI AAT'ATT
Cuts at: 1152 1152
Size: 4192
StuI AGG'CCT
Cuts at: 316 316
Size: 4192
StyI C'CwwG_G
Cuts at: 125 158 1324 1678 125
Size: 33 1166 354 2639
Fragments arranged by size:
2639 1166 354 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 431 1773 1906 2365 2576 431
Size: 1342 133 459 211 2047
Fragments arranged by size:
2047 1342 459 211 133
TatI w'GTAC_w
Cuts at: 3707 3707
Size: 4192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 723 755 3709 3741 723
Size: 32 2954 32 1174
Fragments arranged by size:
2954 1174 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4192
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1685 132
Size: 1553 2639
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4192
XmnI GAAnn'nnTTC
Cuts at: 966 966
Size: 4192
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NruI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NotI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI