Restriction map for pF1KSDA1135
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BspEI       BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CpoI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRV       FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1422  GTCGAC               GT^[AC][GT]AC
AflIII       2210  ACATGT               A^C[GA][CT]GT
Alw44I        665  GTGCAC               G^TGCAC
ApaI         2177  GGGCCC               GGGCC^C
ApaLI         665  GTGCAC               G^TGCAC
BlpI          117  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      117  GCTGAGC              GC^T[ACGT]AGC
BsmI         1660  GAATGC               GAATGC(1/-1)
Bsp1407I      143  TGTACA               T^GTACA
BspDI         469  ATCGAT               AT^CGAT
BspLU11I     2210  ACATGT               A^CATGT
BspMI        1332  GCAGGT               ACCTGC(4/8)
BsrGI         143  TGTACA               T^GTACA
BssHII        207  GCGCGC               G^CGCGC
BstXI         482  CCAACACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         117  GCTGAGC              GC^T[ACGT]AGC
ClaI          469  ATCGAT               AT^CGAT
DraI         1050  TTTAAA               TTT^AAA
DraIII        238  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          277  GACGGCGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1707  CCTGGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1642  GAATTC               G^AATTC
EcoT14I      2093  CCATGG               C^C[AT][AT]GG
EcoT22I      1690  ATGCAT               ATGCA^T
HincII       1422  GTCGAC               GT[CT]^[GA]AC
HindII       1422  GTCGAC               GT[CT]^[GA]AC
MslI         2094  CATGGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         2093  CCATGG               C^CATGG
NsiI         1690  ATGCAT               ATGCA^T
PflMI        1701  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          527  CTGCAG               CTGCA^G
SacI          688  GAGCTC               GAGCT^C
SalI         1422  GTCGAC               G^TCGAC
ScaI         2234  AGTACT               AGT^ACT
SfcI          527  CTGCAG               C^T[GA][CT]AG
SspBI         143  TGTACA               T^GTACA
SspI         1116  AATATT               AAT^ATT
StuI          537  AGGCCT               AGG^CCT
StyI         2093  CCATGG               C^C[AT][AT]GG
Van91I       1701  CCAGGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2066  CCAAAGAGTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AcsI         1504  AAATTC               [GA]^AATT[CT]
             1642  GAATTC              
AcyI          280  GGCGCC               G[GA]^CG[CT]C
              294  GGCGCC              
AflII         733  CTTAAG               C^TTAAG
              856  CTTAAG              
AlwNI        1600  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1702  CAGGTCCTG           
ApoI         1504  AAATTC               [GA]^AATT[CT]
             1642  GAATTC              
AvaI           76  CCCGGG               C^[CT]CG[GA]G
              107  CCCGGG              
BalI          479  TGGCCA               TGG^CCA
             2165  TGGCCA              
BbeI          280  GGCGCC               GGCGC^C
              294  GGCGCC              
BfaI          457  CTAG                 C^TAG
              770  CTAG                
BfrI          733  CTTAAG               C^TTAAG
              856  CTTAAG              
BglI           17  GCCCGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1611  GCCTTCCAGGC         
BsaHI         280  GGCGCC               G[GA]^CG[CT]C
              294  GGCGCC              
BsgI         1340  CTGCAC               GTGCAG(16/14)
             1414  CTGCAC              
BsmBI        1539  GAGACG               CGTCTC(1/5)
             1557  GAGACG              
BsoBI          76  CCCGGG               C^[CT]CG[GA]G
              107  CCCGGG              
BspHI         596  TCATGA               T^CATGA
             1011  TCATGA              
BsrBI        1629  CCGCTC               CCGCTC(-3/-3)
              871  GAGCGG              
BstAPI        667  GCACTGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1599  GCAGTTCCTGC         
BstF5I       1020  CATCC                GGATG(2/0)
              930  GGATG               
FokI         1020  CATCC                GGATG(9/13)
              930  GGATG               
HaeII         280  GGCGCC               [GA]GCGC^[CT]
              294  GGCGCC              
Hin1I         280  GGCGCC               G[GA]^CG[CT]C
              294  GGCGCC              
KasI          280  GGCGCC               G^GCGCC
              294  GGCGCC              
MaeI          457  CTAG                 C^TAG
              770  CTAG                
MaeII        1176  ACGT                 A^CGT
             1592  ACGT                
MaeIII        286  GTCAC                ^GT[ACGT]AC
             1234  GTGAC               
MluNI         479  TGGCCA               TGG^CCA
             2165  TGGCCA              
MscI          479  TGGCCA               TGG^CCA
             2165  TGGCCA              
NaeI           85  GCCGGC               GCC^GGC
             2147  GCCGGC              
NarI          280  GGCGCC               GG^CGCC
              294  GGCGCC              
NgoMIV         85  GCCGGC               G^CCGGC
             2147  GCCGGC              
NspI         1205  GCATGT               [GA]CATG^[CT]
             2210  ACATGT              
PleI         1267  GAGTC                GAGTC(4/5)
             1396  GAGTC               
PpuMI        1703  AGGTCCT              [GA]G^G[AT]CC[CT]
             1923  GGGACCT             
RcaI          596  TCATGA               T^CATGA
             1011  TCATGA              
SapI          784  GAAGAGC              GCTCTTC(1/4)
             1459  GAAGAGC             
SfoI          280  GGCGCC               GGC^GCC
              294  GGCGCC              
SmaI           76  CCCGGG               CCC^GGG
              107  CCCGGG              
Tsp45I        286  GTCAC                ^GT[GC]AC
             1234  GTGAC               
XmaCI          76  CCCGGG               C^CCGGG
              107  CCCGGG              
XmaI           76  CCCGGG               C^CCGGG
              107  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      FauI        
Fnu4HI      HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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