Restriction map for pF1KSDA1093
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BclI        
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       Bsp1407I    BspEI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     CpoI        Eam1105I    EcoNI       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KspI        MamI        MluI        MroI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       Sse8387I    SspBI       SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       3717  GGTACC               G^GTACC
AccII         278  CGCG                 CG^CG
AcyI         2204  GACGCC               G[GA]^CG[CT]C
AflII        1146  CTTAAG               C^TTAAG
AflIII       4778  ACATGT               A^C[GA][CT]GT
Asp718I      3717  GGTACC               G^GTACC
BciVI        4130  GTATCC               GTATCC(6/5)
BfrI         1146  CTTAAG               C^TTAAG
BglI          161  GCCCAATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1678  AGATCT               A^GATCT
BsaHI        2204  GACGCC               G[GA]^CG[CT]C
BsmBI        2422  GAGACG               CGTCTC(1/5)
BsmI         1178  GAATGC               GAATGC(1/-1)
BspDI        3182  ATCGAT               AT^CGAT
BspHI        3170  TCATGA               T^CATGA
BspLU11I     4778  ACATGT               A^CATGT
BsrFI        4918  ACCGGC               [GA]^CCGG[CT]
BstUI         278  CGCG                 CG^CG
Cfr10I       4918  ACCGGC               [GA]^CCGG[CT]
ClaI         3182  ATCGAT               AT^CGAT
DraI         3853  TTTAAA               TTT^AAA
EagI          152  CGGCCG               C^GGCCG
EclXI         152  CGGCCG               C^GGCCG
Eco52I        152  CGGCCG               C^GGCCG
HgaI         2204  GACGC                GACGC(5/10)
Hin1I        2204  GACGCC               G[GA]^CG[CT]C
HincII       4650  GTCAAC               GT[CT]^[GA]AC
HindII       4650  GTCAAC               GT[CT]^[GA]AC
KpnI         3717  GGTACC               GGTAC^C
MvnI          278  CGCG                 CG^CG
PaeR7I       2759  CTCGAG               C^TCGAG
RcaI         3170  TCATGA               T^CATGA
SmaI         3950  CCCGGG               CCC^GGG
SpeI          706  ACTAGT               A^CTAGT
StuI         1921  AGGCCT               AGG^CCT
XhoI         2759  CTCGAG               C^TCGAG
XmaCI        3950  CCCGGG               C^CCGGG
XmaI         3950  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          574  GTAGAC               GT^[AC][GT]AC
             1434  GTCTAC              
AclI         2548  AACGTT               AA^CGTT
             4327  AACGTT              
Alw44I        998  GTGCAC               G^TGCAC
             3623  GTGCAC              
ApaLI         998  GTGCAC               G^TGCAC
             3623  GTGCAC              
AspI         1303  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACTGGGTC           
BalI          931  TGGCCA               TGG^CCA
             4005  TGGCCA              
BbsI           40  GAAGAC               GAAGAC(2/6)
             2226  GTCTTC              
BlpI         3474  GCTGAGC              GC^T[ACGT]AGC
             4855  GCTCAGC             
Bpu1102I     3474  GCTGAGC              GC^T[ACGT]AGC
             4855  GCTCAGC             
BpuAI          40  GAAGAC               GAAGAC(2/6)
             2226  GTCTTC              
BsiEI         152  CGGCCG               CG[GA][CT]^CG
             1771  CGGTCG              
CelII        3474  GCTGAGC              GC^T[ACGT]AGC
             4855  GCTCAGC             
DrdI         2842  GACGGGCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4156  GACCCCTATGTC        
EcoRI        2736  GAATTC               G^AATTC
             4102  GAATTC              
EcoRV        3166  GATATC               GAT^ATC
             3979  GATATC              
MfeI          164  CAATTG               C^AATTG
             2277  CAATTG              
MluNI         931  TGGCCA               TGG^CCA
             4005  TGGCCA              
MscI          931  TGGCCA               TGG^CCA
             4005  TGGCCA              
MunI          164  CAATTG               C^AATTG
             2277  CAATTG              
NcoI         1163  CCATGG               C^CATGG
             4120  CCATGG              
PflFI        1303  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACTGGGTC           
Psp1406I     2548  AACGTT               AA^CGTT
             4327  AACGTT              
SacI         2044  GAGCTC               GAGCT^C
             2858  GAGCTC              
SapI          681  GAAGAGC              GCTCTTC(1/4)
             2626  GAAGAGC             
SexAI        4284  ACCTGGT              A^CC[AT]GGT
             5130  ACCTGGT             
SphI          344  GCATGC               GCATG^C
             4670  GCATGC              
Tth111I      1303  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACTGGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcgI        BcnI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco47III    
Eco81I      EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI        
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