Restriction map for pF1KSDA1059
 (Circular) (Six-base) MAPSORT of: pF1KSDA1059.seq  Check: 5433  from: 1  to: 6803



pF1KSDA1059  6803 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    420    1243    1787    2249     420 
   Size:        823     544     462    4974

  Fragments arranged by size: 

                4974     823     544     462
 
AccI GT'mk_AC

Cuts at:    202     823    3841     202 
   Size:        621    3018    3164

  Fragments arranged by size: 

                3164    3018     621
 
Acc65I G'GTAC_C

Cuts at:   3819    3819 
   Size:       6803
 
AclI AA'CG_TT

Cuts at:   6643    6643 
   Size:       6803
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    680    2133    2184    2724    3272    3436    4628    5060    5807 
   Size:       1453      51     540     548     164    1192     432     747

Cuts at:   5807    6075     680 
   Size:        268    1408

  Fragments arranged by size: 

                1453    1408    1192     747     548     540     432     268
                 164      51
 
AflIII A'CryG_T

Cuts at:   1367    1496    1522    2768    4155    5259    6236    1367 
   Size:        129      26    1246    1387    1104     977    1934

  Fragments arranged by size: 

                1934    1387    1246    1104     977     129      26
 
AgeI A'CCGG_T

Cuts at:   5191    5191 
   Size:       6803
 
AhdI GACnn_n'nnGTC

Cuts at:    248     248 
   Size:       6803
 
AlwNI CAG_nnn'CTG

Cuts at:    490    1023    4108    5675     490 
   Size:        533    3085    1567    1618

  Fragments arranged by size: 

                3085    1618    1567     533
 
ApaLI G'TGCA_C

Cuts at:    727    1189    1741    5573     727 
   Size:        462     552    3832    1957

  Fragments arranged by size: 

                3832    1957     552     462
 
ApoI r'AATT_y

Cuts at:   3396    3807    6156    6412    3396 
   Size:        411    2349     256    3787

  Fragments arranged by size: 

                3787    2349     411     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6803
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6803
 
AvaI C'yCGr_G

Cuts at:    617    2588    3823     617 
   Size:       1971    1235    3597

  Fragments arranged by size: 

                3597    1971    1235
 
BamHI G'GATC_C

Cuts at:   1241    3828    1241 
   Size:       2587    4216
 
BanI G'GyrC_C

Cuts at:    168     220     371     633     835    1697    1978    2237    3819 
   Size:         52     151     262     202     862     281     259    1582

Cuts at:   3819    4233    4481    4516     168 
   Size:        414     248      35    2455

  Fragments arranged by size: 

                2455    1582     862     414     281     262     259     248
                 202     151      52      35
 
BanII G_rGCy'C

Cuts at:    191    1877    2100    2310    3222    3817    4847     191 
   Size:       1686     223     210     912     595    1030    2147

  Fragments arranged by size: 

                2147    1686    1030     912     595     223     210
 
BbeI G_GCGC'C

Cuts at:    172     224     375     637    1701    4237    4485     172 
   Size:         52     151     262    1064    2536     248    2490

  Fragments arranged by size: 

                2536    2490    1064     262     248     151      52
 
BbsI GAAGACnn'nnnn_

Cuts at:    668     880    1110    1669     668 
   Size:        212     230     559    5802

  Fragments arranged by size: 

                5802     559     230     212
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    669     703     669 
   Size:         34    6769
 
BciVI GTATCCnnnnn_n'

Cuts at:   4701    5462    6803    4701 
   Size:        761    1341    4701

  Fragments arranged by size: 

                4701    1341     761
 
BclI T'GATC_A

Cuts at:    962    4323     962 
   Size:       3361    3442
 
BfrBI ATG'CAT

Cuts at:   1122    1122 
   Size:       6803
 
BglI GCCn_nnn'nGGC

Cuts at:    155    1987    2016     155 
   Size:       1832      29    4942

  Fragments arranged by size: 

                4942    1832      29
 
BglII A'GATC_T

Cuts at:   1934    4318    6355    1934 
   Size:       2384    2037    2382

  Fragments arranged by size: 

                2384    2382    2037
 
BlpI GC'TnA_GC

Cuts at:   3498    3915    3498 
   Size:        417    6386
 
Bme1580I G_kGCm'C

Cuts at:    731    1193    1745    1983    2319    2575    2642    3491    4428 
   Size:        462     552     238     336     256      67     849     937

Cuts at:   4428    4521    5577     731 
   Size:         93    1056    1957

  Fragments arranged by size: 

                1957    1056     937     849     552     462     336     256
                 238      93      67
 
BmgBI CAC'GTC

Cuts at:    206     206 
   Size:       6803
 
BmrI ACTGGGnnnn_n'

Cuts at:   1059    1732    1980    2507    2936    2959    4015    4096    4431 
   Size:        673     248     527     429      23    1056      81     335

Cuts at:   4431    6575    1059 
   Size:       2144    1287

  Fragments arranged by size: 

                2144    1287    1056     673     527     429     335     248
                  81      23
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2064    5170    2064 
   Size:       3106    3697
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3462    3462 
   Size:       6803
 
Bpu10I CC'TnA_GC

Cuts at:    608    2539     608 
   Size:       1931    4872
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3581    3979    4858    5350    5648    3581 
   Size:        398     879     492     298    4736

  Fragments arranged by size: 

                4736     879     492     398     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2113    2321    2352    3283    6507    2113 
   Size:        208      31     931    3224    2409

  Fragments arranged by size: 

                3224    2409     931     208      31
 
BsaAI yAC'GTr

Cuts at:   1368    1499    2769    3795    4156    1368 
   Size:        131    1270    1026     361    4015

  Fragments arranged by size: 

                4015    1270    1026     361     131
 
BsaBI GATnn'nnATC

Cuts at:      6    3586    4317    6354       6 
   Size:       3580     731    2037     455

  Fragments arranged by size: 

                3580    2037     731     455
 
BsaHI Gr'CG_yC

Cuts at:    169     221     258     343     372     557     634    1698    4234 
   Size:         52      37      85      29     185      77    1064    2536

Cuts at:   4234    4482    5184    6680     169 
   Size:        248     702    1496     292

  Fragments arranged by size: 

                2536    1496    1064     702     292     248     185      85
                  77      52      37      29
 
BsaWI w'CCGG_w

Cuts at:    179    1713    3992    4513    5191    5465    5612    6023     179 
   Size:       1534    2279     521     678     274     147     411     959

  Fragments arranged by size: 

                2279    1534     959     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    328     358    2404    2434    3447    3477     328 
   Size:         30    2046      30    1013      30    3654

  Fragments arranged by size: 

                3654    2046    1013      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    349     525     528     637    1896    2683    3345    3706     349 
   Size:        176       3     109    1259     787     662     361    3446

  Fragments arranged by size: 

                3446    1259     787     662     361     176     109       3
            
 
BseYI C'CCAG_C

Cuts at:    366     398     483     643     676    2427    3073    3131    3484 
   Size:         32      85     160      33    1751     646      58     353

Cuts at:   3484    4228    4986    5563    6059     366 
   Size:        744     758     577     496    1110

  Fragments arranged by size: 

                1751    1110     758     744     646     577     496     353
                 160      85      58      33      32
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1759    2511    2948    3765    1759 
   Size:        752     437     817    4797

  Fragments arranged by size: 

                4797     817     752     437
 
BsiEI CG_ry'CG

Cuts at:    104     578    2612    4345    4391    5599     104 
   Size:        474    2034    1733      46    1208    1308

  Fragments arranged by size: 

                2034    1733    1308    1208     474      46
 
BsiHKAI G_wGCw'C

Cuts at:    731    1193    1745    1877    2310    3222    3817    4595    4785 
   Size:        462     552     132     433     912     595     778     190

Cuts at:   4785    5577     731 
   Size:        792    1957

  Fragments arranged by size: 

                1957     912     792     778     595     552     462     433
                 190     132
 
BsmI GAATG_Cn'

Cuts at:   3084    3084 
   Size:       6803
 
Bsp1286I G_dGCh'C

Cuts at:    191     731    1193    1745    1877    1983    2100    2310    2319 
   Size:        540     462     552     132     106     117     210       9

Cuts at:   2319    2575    2642    3222    3491    3817    4428    4521    4595 
   Size:        256      67     580     269     326     611      93      74

Cuts at:   4595    4785    4847    5577     191 
   Size:        190      62     730    1417

  Fragments arranged by size: 

                1417     730     611     580     552     540     462     326
                 269     256     210     190     132     117     106      93
                  74      67      62       9
 
BspEI T'CCGG_A

Cuts at:    179    1713    6023     179 
   Size:       1534    4310     959

  Fragments arranged by size: 

                4310    1534     959
 
BspHI T'CATG_A

Cuts at:   2129    3114    5979    2129 
   Size:        985    2865    2953

  Fragments arranged by size: 

                2953    2865     985
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    420    1243    1603    1787    2249    3853    4067    4369    4750 
   Size:        823     360     184     462    1604     214     302     381

Cuts at:   4750     420 
   Size:       2473

  Fragments arranged by size: 

                2473    1604     823     462     381     360     302     214
                 184
 
BsrBI CCG'CTC

Cuts at:     50     465    1286    2159    5095    5149    6633    6798      50 
   Size:        415     821     873    2936      54    1484     165      55

  Fragments arranged by size: 

                2936    1484     873     821     415     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   2208    3210    3260    3348    3734    4715    2208 
   Size:       1002      50      88     386     981    4296

  Fragments arranged by size: 

                4296    1002     981     386      88      50
 
BsrFI r'CCGG_y

Cuts at:    217     285     453    2204    4801    4982    5191     217 
   Size:         68     168    1751    2597     181     209    1829

  Fragments arranged by size: 

                2597    1829    1751     209     181     168      68
 
BssHII G'CGCG_C

Cuts at:    136     136 
   Size:       6803
 
BssSI C'ACGA_G

Cuts at:   1984    5074    5432    1984 
   Size:       3090     358    3355

  Fragments arranged by size: 

                3355    3090     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1119    1749    4180    1119 
   Size:        630    2431    3742

  Fragments arranged by size: 

                3742    2431     630
 
BstBI TT'CG_AA

Cuts at:    110    5164     110 
   Size:       5054    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2435    2949    2435 
   Size:        514    6289
 
BstYI r'GATC_y

Cuts at:      1    1241    1934    2786    3254    3770    3828    4295    4318 
   Size:       1240     693     852     468     516      58     467      23

Cuts at:   4318    4653    4899    5911    5997    6009    6355       1 
   Size:        335     246    1012      86      12     346     449

  Fragments arranged by size: 

                1240    1012     852     693     516     468     467     449
                 346     335     246      86      58      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1806    3689    1806 
   Size:       1883    4920
 
BtgI C'CryG_G

Cuts at:    125     307     637     722    1091    1208    1547     125 
   Size:        182     330      85     369     117     339    5381

  Fragments arranged by size: 

                5381     369     339     330     182     117      85
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4753    4934    5097    4753 
   Size:        181     163    6459

  Fragments arranged by size: 

                6459     181     163
 
BtsI GCAGTG_nn'

Cuts at:    320     566    1743    4046     320 
   Size:        246    1177    2303    3077

  Fragments arranged by size: 

                3077    2303    1177     246
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    272     307     272 
   Size:         35    6768
 
DraI TTT'AAA

Cuts at:   3564    3802    3564 
   Size:        238    6565
 
DraIII CAC_nnn'GTG

Cuts at:   3673    3673 
   Size:       6803
 
DrdI GACnn_nn'nnGTC

Cuts at:   2836    3721    4509    5367    2836 
   Size:        885     788     858    4272

  Fragments arranged by size: 

                4272     885     858     788
 
EaeI y'GGCC_r

Cuts at:    215     280     317     328     575    1160    1544    1988    2142 
   Size:         65      37      11     247     585     384     444     154

Cuts at:   2142    4388    4562    4953    4980    6325     215 
   Size:       2246     174     391      27    1345     693

  Fragments arranged by size: 

                2246    1345     693     585     444     391     384     247
                 174     154      65      37      27      11
 
EagI C'GGCC_G

Cuts at:    575    4388     575 
   Size:       3813    2990
 
EarI CTCTTCn'nnn_

Cuts at:   1347    1374    1940    2848    4826    5036    1347 
   Size:         27     566     908    1978     210    3114

  Fragments arranged by size: 

                3114    1978     908     566     210      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1195    2523    5011    5321    5467    6613    1195 
   Size:       1328    2488     310     146    1146    1385

  Fragments arranged by size: 

                2488    1385    1328    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1875    2308    3220    3815    1875 
   Size:        433     912     595    4863

  Fragments arranged by size: 

                4863     912     595     433
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    680    2133    2184    2724    3272    3436    3462    4628    5060 
   Size:       1453      51     540     548     164      26    1166     432

Cuts at:   5060    5807    6075     680 
   Size:        747     268    1408

  Fragments arranged by size: 

                1453    1408    1166     747     548     540     432     268
                 164      51      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:    607    2665    3088     607 
   Size:       2058     423    4322

  Fragments arranged by size: 

                4322    2058     423
 
EcoO109I rG'GnC_Cy

Cuts at:    210    2592    2954    3351    3942     210 
   Size:       2382     362     397     591    3071

  Fragments arranged by size: 

                3071    2382     591     397     362
 
EcoRI G'AATT_C

Cuts at:   3807    3807 
   Size:       6803
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1292    4822    1292 
   Size:       3530    3273
 
FspI TGC'GCA

Cuts at:   4584    4584 
   Size:       6803
 
HaeII r_GCGC'y

Cuts at:    150     172     224     375     637    1701    4237    4485    5507 
   Size:         22      52     151     262    1064    2536     248    1022

Cuts at:   5507    6486     150 
   Size:        979     467

  Fragments arranged by size: 

                2536    1064    1022     979     467     262     248     151
                  52      22
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    234     266     328     360     501     533    2176    2208    2330 
   Size:         32      62      32     141      32    1643      32     122

Cuts at:   2330    2362    3447    3479    4645    4675    4677    4707     234 
   Size:         32    1085      32    1166      30       2      30    2330

  Fragments arranged by size: 

                2330    1643    1166    1085     141     122      62      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    203     824    2091    2919    2947    3842     203 
   Size:        621    1267     828      28     895    3164

  Fragments arranged by size: 

                3164    1267     895     828     621      28
 
HindIII A'AGCT_T

Cuts at:    496     496 
   Size:       6803
 
KasI G'GCGC_C

Cuts at:    168     220     371     633    1697    4233    4481     168 
   Size:         52     151     262    1064    2536     248    2490

  Fragments arranged by size: 

                2536    2490    1064     262     248     151      52
 
KpnI G_GTAC'C

Cuts at:   3823    3823 
   Size:       6803
 
MfeI C'AATT_G

Cuts at:   2170    2370    2170 
   Size:        200    6603
 
MluI A'CGCG_T

Cuts at:   1522    6236    1522 
   Size:       4714    2089
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    928    2543    3627    5474    5658     928 
   Size:       1615    1084    1847     184    2073

  Fragments arranged by size: 

                2073    1847    1615    1084     184
 
MscI TGG'CCA

Cuts at:    319    1546    1990    2144    4564     319 
   Size:       1227     444     154    2420    2558

  Fragments arranged by size: 

                2558    2420    1227     444     154
 
MslI CAynn'nnrTG

Cuts at:   1198    2662    3265    3508    4792    4919    1198 
   Size:       1464     603     243    1284     127    3082

  Fragments arranged by size: 

                3082    1464    1284     603     243     127
 
MspA1I CmG'CkG

Cuts at:    309     639    2836    3453    3914    4228    4588    5601    5846 
   Size:        330    2197     617     461     314     360    1013     245

Cuts at:   5846     309 
   Size:       1266

  Fragments arranged by size: 

                2197    1266    1013     617     461     360     330     314
                 245
 
NaeI GCC'GGC

Cuts at:    219    2206    4984     219 
   Size:       1987    2778    2038

  Fragments arranged by size: 

                2778    2038    1987
 
NarI GG'CG_CC

Cuts at:    169     221     372     634    1698    4234    4482     169 
   Size:         52     151     262    1064    2536     248    2490

  Fragments arranged by size: 

                2536    2490    1064     262     248     151      52
 
NcoI C'CATG_G

Cuts at:    125     722    1091    1547     125 
   Size:        597     369     456    5381

  Fragments arranged by size: 

                5381     597     456     369
 
NgoMIV G'CCGG_C

Cuts at:    217    2204    4982     217 
   Size:       1987    2778    2038

  Fragments arranged by size: 

                2778    2038    1987
 
NsiI A_TGCA'T

Cuts at:   1124    1124 
   Size:       6803
 
NspI r_CATG'y

Cuts at:   1434    2854    2875    3856    4356    5263    1434 
   Size:       1420      21     981     500     907    2974

  Fragments arranged by size: 

                2974    1420     981     907     500      21
 
PasI CC'CwG_GG

Cuts at:   2319    2319 
   Size:       6803
 
PciI A'CATG_T

Cuts at:   5259    5259 
   Size:       6803
 
PflMI CCAn_nnn'nTGG

Cuts at:    326     723    3399     326 
   Size:        397    2676    3730

  Fragments arranged by size: 

                3730    2676     397
 
PfoI T'CCnGG_A

Cuts at:    252    1244    1313    3089     252 
   Size:        992      69    1776    3966

  Fragments arranged by size: 

                3966    1776     992      69
 
PmeI GTTT'AAAC

Cuts at:   3802    3802 
   Size:       6803
 
PmlI CAC'GTG

Cuts at:   2769    2769 
   Size:       6803
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5942    5974    5942 
   Size:         32    6771
 
PpuMI rG'GwC_Cy

Cuts at:    210    2954    3351     210 
   Size:       2744     397    3662

  Fragments arranged by size: 

                3662    2744     397
 
PshAI GACnn'nnGTC

Cuts at:    208     208 
   Size:       6803
 
PstI C_TGCA'G

Cuts at:   1240    2470    3850    4535    1240 
   Size:       1230    1380     685    3508

  Fragments arranged by size: 

                3508    1380    1230     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6803
 
PvuII CAG'CTG

Cuts at:   2836    3453    4228    4588    2836 
   Size:        617     775     360    5051

  Fragments arranged by size: 

                5051     775     617     360
 
RsrII CG'GwC_CG

Cuts at:   4998    4998 
   Size:       6803
 
SacI G_AGCT'C

Cuts at:   1877    2310    3222    3817    1877 
   Size:        433     912     595    4863

  Fragments arranged by size: 

                4863     912     595     433
 
SacII CC_GC'GG

Cuts at:    310     640     310 
   Size:        330    6473
 
SalI G'TCGA_C

Cuts at:    201     822    3840     201 
   Size:        621    3018    3164

  Fragments arranged by size: 

                3164    3018     621
 
SanDI GG'GwC_CC

Cuts at:   2954    2954 
   Size:       6803
 
SapI GCTCTTCn'nnn_

Cuts at:   1347    4826    5036    1347 
   Size:       3479     210    3114

  Fragments arranged by size: 

                3479    3114     210
 
SbfI CC_TGCA'GG

Cuts at:   1240    3850    1240 
   Size:       2610    4193
 
ScaI AGT'ACT

Cuts at:   1414    6320    1414 
   Size:       4906    1897
 
SexAI A'CCwGG_T

Cuts at:   1184    1655    1184 
   Size:        471    6332
 
SfcI C'TryA_G

Cuts at:     33    1062    1083    1236    1853    2466    3846    4531    5524 
   Size:       1029      21     153     617     613    1380     685     993

Cuts at:   5524    5715      33 
   Size:        191    1121

  Fragments arranged by size: 

                1380    1121    1029     993     685     617     613     191
                 153      21
 
SfoI GGC'GCC

Cuts at:    170     222     373     635    1699    4235    4483     170 
   Size:         52     151     262    1064    2536     248    2490

  Fragments arranged by size: 

                2536    2490    1064     262     248     151      52
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6803
 
SgrAI Cr'CCGG_yG

Cuts at:    285     285 
   Size:       6803
 
SmaI CCC'GGG

Cuts at:    619    3825     619 
   Size:       3206    3597
 
SmlI C'TyrA_G

Cuts at:   3596    3958    4873    5365    5627    3596 
   Size:        362     915     492     262    4772

  Fragments arranged by size: 

                4772     915     492     362     262
 
SnaBI TAC'GTA

Cuts at:   3795    3795 
   Size:       6803
 
SpeI A'CTAG_T

Cuts at:     67    1345    3784      67 
   Size:       1278    2439    3086

  Fragments arranged by size: 

                3086    2439    1278
 
SphI G_CATG'C

Cuts at:   3856    4356    3856 
   Size:        500    6303
 
SspI AAT'ATT

Cuts at:   2285    3121    2285 
   Size:        836    5967
 
StuI AGG'CCT

Cuts at:   1387    1387 
   Size:       6803
 
StyI C'CwwG_G

Cuts at:    125     722    1091    1547    2216    2472    2558    2820    3577 
   Size:        597     369     456     669     256      86     262     757

Cuts at:   3577    3937    4291     125 
   Size:        360     354    2637

  Fragments arranged by size: 

                2637     757     669     597     456     369     360     354
                 262     256      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    556    1041    1152    2016    2626    3327    4386    4519    4978 
   Size:        485     111     864     610     701    1059     133     459

Cuts at:   4978    5187     556 
   Size:        209    2172

  Fragments arranged by size: 

                2172    1059     864     701     610     485     459     209
                 133     111
 
TatI w'GTAC_w

Cuts at:   1412    1444    1949    2549    6318    1412 
   Size:         32     505     600    3769    1897

  Fragments arranged by size: 

                3769    1897     600     505      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1724    1756    1832    1864    2061    2093    2368    2400    2404 
   Size:         32      76      32     197      32     275      32       4

Cuts at:   2404    2436    6320    6352    1724 
   Size:         32    3884      32    2175

  Fragments arranged by size: 

                3884    2175     275     197      76      32      32      32
                  32      32      32       4
 
Tth111I GACn'n_nGTC

Cuts at:   4600    4600 
   Size:       6803
 
XbaI T'CTAG_A

Cuts at:   3834    3834 
   Size:       6803
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2572    2704    3070    4298    2572 
   Size:        132     366    1228    5077

  Fragments arranged by size: 

                5077    1228     366     132
 
XmaI C'CCGG_G

Cuts at:    617    3823     617 
   Size:       3206    3597
 
XmnI GAAnn'nnTTC

Cuts at:   2284    2284 
   Size:       6803
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI     BmtI    BsiWI    BsmBI    BsrGI   BstEII
  BstZ17I     ClaI    EcoRV     FseI    FspAI     HpaI     NdeI     NheI
     NotI     NruI     PacI     PsiI   PspOMI    PspXI     PsrI     SfiI
     SrfI     SwaI     XhoI     ZraI
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