Restriction map for pF1KSDA1058
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AvrII       BbvCI       BlnI        
BsiWI       BspDI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       ClaI        CpoI        
DraIII      Eam1105I    EcoO65I     FseI        KpnI        
KspI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SwaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Aor51HI      5183  AGCGCT               AGC^GCT
ApaI          114  GGGCCC               GGGCC^C
AspI          260  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        5158  TGCGCA               TGC^GCA
BanI         3679  GGCGCC               G^G[CT][GA]CC
BbeI         3679  GGCGCC               GGCGC^C
BbrPI        2581  CACGTG               CAC^GTG
BglII         659  AGATCT               A^GATCT
BsaBI        3399  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5900  CGGCCG               CG[GA][CT]^CG
Bsp1407I      539  TGTACA               T^GTACA
BspLU11I     4415  ACATGT               A^CATGT
BsrBI        4409  GAGCGG               CCGCTC(-3/-3)
BsrGI         539  TGTACA               T^GTACA
Bst1107I     2460  GTATAC               GTA^TAC
BstZ17I      2460  GTATAC               GTA^TAC
DraI         4341  TTTAAA               TTT^AAA
EagI         5900  CGGCCG               C^GGCCG
EclXI        5900  CGGCCG               C^GGCCG
Eco47III     5183  AGCGCT               AGC^GCT
Eco52I       5900  CGGCCG               C^GGCCG
EcoRI        2002  GAATTC               G^AATTC
EcoRV        6017  GATATC               GAT^ATC
FspI         5158  TGCGCA               TGC^GCA
HpaI         2662  GTTAAC               GTT^AAC
KasI         3679  GGCGCC               G^GCGCC
MamI         3399  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1760  CAATTG               C^AATTG
MluI         2747  ACGCGT               A^CGCGT
MunI         1760  CAATTG               C^AATTG
NaeI          123  GCCGGC               GCC^GGC
NarI         3679  GGCGCC               GG^CGCC
NcoI         4263  CCATGG               C^CATGG
NgoMIV        123  GCCGGC               G^CCGGC
PaeR7I       6124  CTCGAG               C^TCGAG
PflFI         260  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2581  CACGTG               CAC^GTG
PmlI         2581  CACGTG               CAC^GTG
SalI          472  GTCGAC               G^TCGAC
SexAI        2058  ACCTGGT              A^CC[AT]GGT
SfoI         3679  GGCGCC               GGC^GCC
SmaI          111  CCCGGG               CCC^GGG
SspBI         539  TGTACA               T^GTACA
Tth111I       260  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1749  TCTAGA               T^CTAGA
XcmI         4869  CCATGTCAAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         6124  CTCGAG               C^TCGAG
XmaCI         111  CCCGGG               C^CCGGG
XmaI          111  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII       2391  TCCGGA               T^CCGGA
             4550  TCCGGA              
AcyI         3627  GACGCC               G[GA]^CG[CT]C
             3679  GGCGCC              
AflIII       2747  ACGCGT               A^C[GA][CT]GT
             4415  ACATGT              
Asp700I      1257  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1382  GAATATTTTC          
BcgI          268  CGATACATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5190  CGAGCTCATTGC        
BciVI        4721  GGATAC               GTATCC(6/5)
             1368  GTATCC              
BclI         2897  TGATCA               T^GATCA
             5915  TGATCA              
BsaHI        3627  GACGCC               G[GA]^CG[CT]C
             3679  GGCGCC              
BsaI         3164  GAGACC               GGTCTC(1/5)
             2325  GGTCTC              
BseAI        2391  TCCGGA               T^CCGGA
             4550  TCCGGA              
BsmBI         255  GAGACG               CGTCTC(1/5)
              591  GAGACG              
BspEI        2391  TCCGGA               T^CCGGA
             4550  TCCGGA              
BspHI        1350  TCATGA               T^CATGA
             4280  TCATGA              
BsrFI         123  GCCGGC               [GA]^CCGG[CT]
             4667  ACCGGC              
Cfr10I        123  GCCGGC               [GA]^CCGG[CT]
             4667  ACCGGC              
DsaI         3860  CCGTGG               C^C[GA][CT]GG
             4263  CCATGG              
EcoNI        3660  CCTGCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5000  CCTGCCTGAGG         
FauI         3571  GCGGG                CCCGC(4/6)
             5726  GCGGG               
FbaI         2897  TGATCA               T^GATCA
             5915  TGATCA              
HgaI         3627  GACGC                GACGC(5/10)
             4144  GACGC               
Hin1I        3627  GACGCC               G[GA]^CG[CT]C
             3679  GGCGCC              
HindIII      1036  AAGCTT               A^AGCTT
             6071  AAGCTT              
MroI         2391  TCCGGA               T^CCGGA
             4550  TCCGGA              
RcaI         1350  TCATGA               T^CATGA
             4280  TCATGA              
StuI         4484  AGGCCT               AGG^CCT
             5081  AGGCCT              
XmnI         1257  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1382  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflII       AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BalI        
BamHI       BanII       BbsI        BbvI        BcnI        
BfaI        BfrI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DrdI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
EcoT22I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflMI       PleI        
PpuMI       PshAI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SphI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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