Restriction map for pF1KSDA1045
 (Linear) (Six-base) MAPSORT of: KIAA1045.seq  Check: 7886  from: 1  to: 1200



KIAA1045.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      27    1200 
   Size:         27    1173
 
AhdI GACnn_n'nnGTC

Cuts at:      0     804    1200 
   Size:        804     396
 
AleI CACnn'nnGTG

Cuts at:      0     560    1200 
   Size:        560     640
 
AlwNI CAG_nnn'CTG

Cuts at:      0     446    1200 
   Size:        446     754
 
ApaI G_GGCC'C

Cuts at:      0     717     957    1200 
   Size:        717     240     243

  Fragments arranged by size: 

                 717     243     240
 
AvaI C'yCGr_G

Cuts at:      0     893     911    1200 
   Size:        893      18     289

  Fragments arranged by size: 

                 893     289      18
 
BanII G_rGCy'C

Cuts at:      0     151     368     717     894     957    1200 
   Size:        151     217     349     177      63     243

  Fragments arranged by size: 

                 349     243     217     177     151      63
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     176     210    1200 
   Size:        176      34     990

  Fragments arranged by size: 

                 990     176      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     485     907    1200 
   Size:        485     422     293

  Fragments arranged by size: 

                 485     422     293
 
Bme1580I G_kGCm'C

Cuts at:      0     717     957    1200 
   Size:        717     240     243

  Fragments arranged by size: 

                 717     243     240
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     382     567     679    1200 
   Size:        332      50     185     112     521

  Fragments arranged by size: 

                 521     332     185     112      50
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     607    1200 
   Size:        607     593
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     583     613    1200 
   Size:        583      30     587

  Fragments arranged by size: 

                 587     583      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     184     286     604     739    1200 
   Size:        184     102     318     135     461

  Fragments arranged by size: 

                 461     318     184     135     102
 
BseYI C'CCAG_C

Cuts at:      0     956    1044    1200 
   Size:        956      88     156

  Fragments arranged by size: 

                 956     156      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     432     459     527    1200 
   Size:         61     371      27      68     673

  Fragments arranged by size: 

                 673     371      68      61      27
 
BsiEI CG_ry'CG

Cuts at:      0     225    1200 
   Size:        225     975
 
BsiHKAI G_wGCw'C

Cuts at:      0     928    1200 
   Size:        928     272
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     368     717     894     928     957    1200 
   Size:        151     217     349     177      34      29     243

  Fragments arranged by size: 

                 349     243     217     177     151      34      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      27    1200 
   Size:         27    1173
 
BsrBI CCG'CTC

Cuts at:      0     240     882    1200 
   Size:        240     642     318

  Fragments arranged by size: 

                 642     318     240
 
BsrFI r'CCGG_y

Cuts at:      0     220    1200 
   Size:        220     980
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     446     837    1200 
   Size:        446     391     363

  Fragments arranged by size: 

                 446     391     363
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1110    1200 
   Size:       1110      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     269    1200 
   Size:        269     931
 
BtgI C'CryG_G

Cuts at:      0     132    1200 
   Size:        132    1068
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1014    1200 
   Size:       1014     186
 
BtsI GCAGTG_nn'

Cuts at:      0     924    1008    1200 
   Size:        924      84     192

  Fragments arranged by size: 

                 924     192      84
 
EaeI y'GGCC_r

Cuts at:      0     222    1200 
   Size:        222     978
 
EagI C'GGCC_G

Cuts at:      0     222    1200 
   Size:        222     978
 
EarI CTCTTCn'nnn_

Cuts at:      0     203     436     728    1200 
   Size:        203     233     292     472

  Fragments arranged by size: 

                 472     292     233     203
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     304    1200 
   Size:        304     896
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     382     567     679    1200 
   Size:        332      50     185     112     521

  Fragments arranged by size: 

                 521     332     185     112      50
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     721    1200 
   Size:        721     479
 
EcoO109I rG'GnC_Cy

Cuts at:      0     125     713     714    1024    1200 
   Size:        125     588       1     310     176

  Fragments arranged by size: 

                 588     310     176     125       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     790     822    1084    1116    1200 
   Size:        790      32     262      32      84

  Fragments arranged by size: 

                 790     262      84      32      32
 
HincII GTy'rAC

Cuts at:      0     384    1200 
   Size:        384     816
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     126    1200 
   Size:        126    1074
 
MslI CAynn'nnrTG

Cuts at:      0     560    1200 
   Size:        560     640
 
MspA1I CmG'CkG

Cuts at:      0     421     511     624    1200 
   Size:        421      90     113     576

  Fragments arranged by size: 

                 576     421     113      90
 
NaeI GCC'GGC

Cuts at:      0     222    1200 
   Size:        222     978
 
NgoMIV G'CCGG_C

Cuts at:      0     220    1200 
   Size:        220     980
 
PfoI T'CCnGG_A

Cuts at:      0     155     203    1200 
   Size:        155      48     997

  Fragments arranged by size: 

                 997     155      48
 
PpuMI rG'GwC_Cy

Cuts at:      0     125    1024    1200 
   Size:        125     899     176

  Fragments arranged by size: 

                 899     176     125
 
PspOMI G'GGCC_C

Cuts at:      0     713     953    1200 
   Size:        713     240     247

  Fragments arranged by size: 

                 713     247     240
 
PstI C_TGCA'G

Cuts at:      0     833    1003    1200 
   Size:        833     170     197

  Fragments arranged by size: 

                 833     197     170
 
PvuII CAG'CTG

Cuts at:      0     421    1200 
   Size:        421     779
 
SanDI GG'GwC_CC

Cuts at:      0     125    1024    1200 
   Size:        125     899     176

  Fragments arranged by size: 

                 899     176     125
 
SapI GCTCTTCn'nnn_

Cuts at:      0     728    1200 
   Size:        728     472
 
SfcI C'TryA_G

Cuts at:      0     140     829     999    1200 
   Size:        140     689     170     201

  Fragments arranged by size: 

                 689     201     170     140
 
StuI AGG'CCT

Cuts at:      0      93    1200 
   Size:         93    1107
 
StyI C'CwwG_G

Cuts at:      0     495    1200 
   Size:        495     705
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214    1200 
   Size:        182      32     986

  Fragments arranged by size: 

                 986     182      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     327    1200 
   Size:        327     873
 

 Enzymes that do cut:

     AarI     AhdI     AleI    AlwNI     ApaI     AvaI    BanII     BcgI
     BglI Bme1580I     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI    BstXI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MmeI     MslI   MspA1I
     NaeI   NgoMIV     PfoI    PpuMI   PspOMI     PstI    PvuII    SanDI
     SapI     SfcI     StuI     StyI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstYI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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