Restriction map for
pF1KSDA1044
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NotI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 850 GACGTC GACGT^C
AclI 179 AACGTT AA^CGTT
AcsI 288 GAATTT [GA]^AATT[CT]
AcyI 850 GACGTC G[GA]^CG[CT]C
AlwNI 1578 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 288 GAATTT [GA]^AATT[CT]
AvaII 110 GGACC G^G[AT]CC
AviII 929 TGCGCA TGC^GCA
BamHI 1295 GGATCC G^GATCC
BmyI 1268 GGGCAC G[AGT]GC[ACT]^C
BsaBI 1674 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 850 GACGTC G[GA]^CG[CT]C
BsaWI 1206 TCCGGT [AT]^CCGG[AT]
BseRI 403 GAGGAG GAGGAG(10/8)
BsmFI 215 GGGAC GGGAC(10/14)
Bsp1286I 1268 GGGCAC G[AGT]GC[ACT]^C
BsrBI 667 GAGCGG CCGCTC(-3/-3)
BsrFI 81 GCCGGC [GA]^CCGG[CT]
BstXI 539 CCAGCACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 81 GCCGGC [GA]^CCGG[CT]
DraI 1725 TTTAAA TTT^AAA
DrdI 1483 GACGAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 995 CCATGG C^C[GA][CT]GG
EcoT14I 995 CCATGG C^C[AT][AT]GG
FspI 929 TGCGCA TGC^GCA
Hin1I 850 GACGTC G[GA]^CG[CT]C
MamI 1674 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 792 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 81 GCCGGC GCC^GGC
NcoI 995 CCATGG C^CATGG
NgoMIV 81 GCCGGC G^CCGGC
NheI 1051 GCTAGC G^CTAGC
NruI 599 TCGCGA TCG^CGA
NspI 1331 ACATGC [GA]CATG^[CT]
PpuMI 109 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 179 AACGTT AA^CGTT
PvuII 1366 CAGCTG CAG^CTG
SbfI 441 CCTGCAGG CCTGCA^GG
ScaI 1853 AGTACT AGT^ACT
SmlI 920 CTCAAG C^T[CT][GA]AG
Sse8387I 441 CCTGCAGG CCTGCA^GG
SspI 1867 AATATT AAT^ATT
StuI 924 AGGCCT AGG^CCT
StyI 995 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1086 GTATAC GT^[AC][GT]AC
1655 GTATAC
AccII 453 CGCG CG^CG
600 CGCG
AvaI 69 CTCGGG C^[CT]CG[GA]G
90 CCCGAG
BalI 803 TGGCCA TGG^CCA
1289 TGGCCA
BanI 145 GGCACC G^G[CT][GA]CC
1128 GGTGCC
BciVI 456 GGATAC GTATCC(6/5)
1216 GTATCC
BsmAI 1829 GAGAC GTCTC(1/5)
589 GTCTC
BsmBI 588 CGTCTC CGTCTC(1/5)
1829 GAGACG
BsoBI 69 CTCGGG C^[CT]CG[GA]G
90 CCCGAG
BspHI 710 TCATGA T^CATGA
782 TCATGA
BspMI 1439 ACCTGC ACCTGC(4/8)
1582 ACCTGC
BsrDI 582 CATTGC GCAATG(2/0)
1191 CATTGC
BssSI 325 CACGAG CACGAG(-5/-1)
1471 CACGAG
Bst1107I 1086 GTATAC GTA^TAC
1655 GTATAC
BstUI 453 CGCG CG^CG
600 CGCG
BstYI 896 GGATCT [GA]^GATC[CT]
1295 GGATCC
BstZ17I 1086 GTATAC GTA^TAC
1655 GTATAC
EaeI 803 TGGCCA [CT]^GGCC[GA]
1289 TGGCCA
EarI 951 GAAGAG CTCTTC(1/4)
1741 GAAGAG
HgaI 451 GACGC GACGC(5/10)
707 GCGTC
Ksp632I 951 GAAGAG CTCTTC(1/4)
1741 GAAGAG
MflI 896 GGATCT [GA]^GATC[CT]
1295 GGATCC
MluNI 803 TGGCCA TGG^CCA
1289 TGGCCA
MscI 803 TGGCCA TGG^CCA
1289 TGGCCA
MvnI 453 CGCG CG^CG
600 CGCG
PleI 878 GAGTC GAGTC(4/5)
1571 GAGTC
RcaI 710 TCATGA T^CATGA
782 TCATGA
TaqI 524 TCGA T^CGA
794 TCGA
Tsp509I 289 AATT ^AATT
968 AATT
TthHB8I 524 TCGA T^CGA
794 TCGA
XhoII 896 GGATCT [GA]^GATC[CT]
1295 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BcnI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I TspRI