Restriction map for pF1KSDA1036
 (Linear) (Six-base) MAPSORT of: KIAA1036.seq  Check: 4921  from: 1  to: 1095



KIAA1036.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:15  ..

AccI GT'mk_AC

Cuts at:      0     343    1095 
   Size:        343     752
 
Acc65I G'GTAC_C

Cuts at:      0    1079    1095 
   Size:       1079      16
 
AfeI AGC'GCT

Cuts at:      0     379     562    1095 
   Size:        379     183     533

  Fragments arranged by size: 

                 533     379     183
 
AflIII A'CryG_T

Cuts at:      0     237    1095 
   Size:        237     858
 
ApaI G_GGCC'C

Cuts at:      0     931     984    1095 
   Size:        931      53     111

  Fragments arranged by size: 

                 931     111      53
 
ApoI r'AATT_y

Cuts at:      0     527    1095 
   Size:        527     568
 
AvaI C'yCGr_G

Cuts at:      0    1029    1095 
   Size:       1029      66
 
BamHI G'GATC_C

Cuts at:      0     281    1095 
   Size:        281     814
 
BanI G'GyrC_C

Cuts at:      0      40    1079    1095 
   Size:         40    1039      16

  Fragments arranged by size: 

                1039      40      16
 
BanII G_rGCy'C

Cuts at:      0     716     931     984    1055    1095 
   Size:        716     215      53      71      40

  Fragments arranged by size: 

                 716     215      71      53      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1048    1095 
   Size:       1048      47
 
BbvCI CC'TCA_GC

Cuts at:      0     111    1095 
   Size:        111     984
 
BlpI GC'TnA_GC

Cuts at:      0     706    1095 
   Size:        706     389
 
Bme1580I G_kGCm'C

Cuts at:      0     931     984    1095 
   Size:        931      53     111

  Fragments arranged by size: 

                 931     111      53
 
BmgBI CAC'GTC

Cuts at:      0     843    1095 
   Size:        843     252
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     978    1095 
   Size:        978     117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     461    1095 
   Size:        461     634
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     901    1095 
   Size:        577     324     194

  Fragments arranged by size: 

                 577     324     194
 
Bpu10I CC'TnA_GC

Cuts at:      0     111     356    1095 
   Size:        111     245     739

  Fragments arranged by size: 

                 739     245     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745     892    1095 
   Size:        745     147     203

  Fragments arranged by size: 

                 745     203     147
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      88    1095 
   Size:         88    1007
 
BsaAI yAC'GTr

Cuts at:      0     240     756    1095 
   Size:        240     516     339

  Fragments arranged by size: 

                 516     339     240
 
BsaHI Gr'CG_yC

Cuts at:      0     170    1095 
   Size:        170     925
 
BsaWI w'CCGG_w

Cuts at:      0     950    1095 
   Size:        950     145
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     254     284     915     945    1095 
   Size:        254      30     631      30     150

  Fragments arranged by size: 

                 631     254     150      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142    1095 
   Size:        142     953
 
BseYI C'CCAG_C

Cuts at:      0      22     618     651     774    1095 
   Size:         22     596      33     123     321

  Fragments arranged by size: 

                 596     321     123      33      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1095 
   Size:        394     701
 
BsiEI CG_ry'CG

Cuts at:      0     744    1095 
   Size:        744     351
 
BsiHKAI G_wGCw'C

Cuts at:      0     716     761    1095 
   Size:        716      45     334

  Fragments arranged by size: 

                 716     334      45
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     155    1095 
   Size:        155     940
 
Bsp1286I G_dGCh'C

Cuts at:      0     716     761     931     984    1055    1095 
   Size:        716      45     170      53      71      40

  Fragments arranged by size: 

                 716     170      71      53      45      40
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     164     308    1095 
   Size:        164     144     787

  Fragments arranged by size: 

                 787     164     144
 
BsrGI T'GTAC_A

Cuts at:      0     680    1095 
   Size:        680     415
 
BssSI C'ACGA_G

Cuts at:      0     715    1095 
   Size:        715     380
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     834    1095 
   Size:        834     261
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     259    1095 
   Size:        259     836
 
BstYI r'GATC_y

Cuts at:      0     248     281     308    1085    1095 
   Size:        248      33      27     777      10

  Fragments arranged by size: 

                 777     248      33      27      10
 
BtgI C'CryG_G

Cuts at:      0     285    1095 
   Size:        285     810
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     875    1095 
   Size:        875     220
 
BtsI GCAGTG_nn'

Cuts at:      0    1020    1095 
   Size:       1020      75
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     174     209    1095 
   Size:        174      35     886

  Fragments arranged by size: 

                 886     174      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     615     807     834    1095 
   Size:        615     192      27     261

  Fragments arranged by size: 

                 615     261     192      27
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     169    1095 
   Size:        169     926
 
EaeI y'GGCC_r

Cuts at:      0      62     242     467     741    1095 
   Size:         62     180     225     274     354

  Fragments arranged by size: 

                 354     274     225     180      62
 
EagI C'GGCC_G

Cuts at:      0     741    1095 
   Size:        741     354
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     143     430    1095 
   Size:        131      12     287     665

  Fragments arranged by size: 

                 665     287     131      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     595    1095 
   Size:        595     500
 
EcoICRI GAG'CTC

Cuts at:      0     714    1095 
   Size:        714     381
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     901    1095 
   Size:        577     324     194

  Fragments arranged by size: 

                 577     324     194
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     222     449     469    1095 
   Size:        222     227      20     626

  Fragments arranged by size: 

                 626     227     222      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     489     672     928    1095 
   Size:        153     336     183     256     167

  Fragments arranged by size: 

                 336     256     183     167     153
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     603    1095 
   Size:        603     492
 
HaeII r_GCGC'y

Cuts at:      0     363     381     564     852     888    1095 
   Size:        363      18     183     288      36     207

  Fragments arranged by size: 

                 363     288     207     183      36      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     915     947    1095 
   Size:        915      32     148

  Fragments arranged by size: 

                 915     148      32
 
HincII GTy'rAC

Cuts at:      0     186    1095 
   Size:        186     909
 
KpnI G_GTAC'C

Cuts at:      0    1083    1095 
   Size:       1083      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1095 
   Size:         73    1022
 
MscI TGG'CCA

Cuts at:      0     244     469    1095 
   Size:        244     225     626

  Fragments arranged by size: 

                 626     244     225
 
MslI CAynn'nnrTG

Cuts at:      0     257    1095 
   Size:        257     838
 
MspA1I CmG'CkG

Cuts at:      0      38      58     746     978    1095 
   Size:         38      20     688     232     117

  Fragments arranged by size: 

                 688     232     117      38      20
 
NspI r_CATG'y

Cuts at:      0     549     903    1095 
   Size:        549     354     192

  Fragments arranged by size: 

                 549     354     192
 
PmlI CAC'GTG

Cuts at:      0     240     756    1095 
   Size:        240     516     339

  Fragments arranged by size: 

                 516     339     240
 
PpuMI rG'GwC_Cy

Cuts at:      0     153     672    1095 
   Size:        153     519     423

  Fragments arranged by size: 

                 519     423     153
 
PspOMI G'GGCC_C

Cuts at:      0     927     980    1095 
   Size:        927      53     115

  Fragments arranged by size: 

                 927     115      53
 
PstI C_TGCA'G

Cuts at:      0     398    1095 
   Size:        398     697
 
SacI G_AGCT'C

Cuts at:      0     716    1095 
   Size:        716     379
 
SapI GCTCTTCn'nnn_

Cuts at:      0     430    1095 
   Size:        430     665
 
SfcI C'TryA_G

Cuts at:      0     394    1095 
   Size:        394     701
 
SmlI C'TyrA_G

Cuts at:      0     760     907    1095 
   Size:        760     147     188

  Fragments arranged by size: 

                 760     188     147
 
SphI G_CATG'C

Cuts at:      0     549    1095 
   Size:        549     546
 
StyI C'CwwG_G

Cuts at:      0     470     944    1095 
   Size:        470     474     151

  Fragments arranged by size: 

                 474     470     151
 
TatI w'GTAC_w

Cuts at:      0     398     680    1095 
   Size:        398     282     415

  Fragments arranged by size: 

                 415     398     282
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     214     246     254     286    1095 
   Size:        214      32       8      32     809

  Fragments arranged by size: 

                 809     214      32      32       8
 
XmnI GAAnn'nnTTC

Cuts at:      0     601    1095 
   Size:        601     494
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrGI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
     KpnI     MmeI     MscI     MslI   MspA1I     NspI     PmlI    PpuMI
   PspOMI     PstI     SacI     SapI     SfcI     SmlI     SphI     StyI
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BmtI    BsaBI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI     DraI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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