Restriction map for pF1KSDA1014
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
CfoI        Cfr10I      CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco47III    EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
MamI        MluI        MroI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I         21  GGTACC               G^GTACC
AseI         1636  ATTAAT               AT^TAAT
AsnI         1636  ATTAAT               AT^TAAT
Asp718I        21  GGTACC               G^GTACC
BalI          434  TGGCCA               TGG^CCA
BanI           21  GGTACC               G^G[CT][GA]CC
BbsI         1138  GAAGAC               GAAGAC(2/6)
BbvCI         472  CCTCAGC              CCTCAGC(-5/-2)
BciVI        2855  GTATCC               GTATCC(6/5)
BpuAI        1138  GAAGAC               GAAGAC(2/6)
BsiEI          27  CGGCCG               CG[GA][CT]^CG
BsmI         1334  GAATGC               GAATGC(1/-1)
BspDI         454  ATCGAT               AT^CGAT
BspHI         278  TCATGA               T^CATGA
BspMI        2604  ACCTGC               ACCTGC(4/8)
BsrDI        2660  CATTGC               GCAATG(2/0)
BstEII       2464  GGTCACC              G^GT[ACGT]ACC
BstPI        2464  GGTCACC              G^GT[ACGT]ACC
BstXI         591  CCAAACATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI          454  ATCGAT               AT^CGAT
EagI           27  CGGCCG               C^GGCCG
EclXI          27  CGGCCG               C^GGCCG
Eco52I         27  CGGCCG               C^GGCCG
EcoO65I      2464  GGTCACC              G^GT[ACGT]ACC
EcoRV        1306  GATATC               GAT^ATC
HincII        411  GTCAAC               GT[CT]^[GA]AC
HindII        411  GTCAAC               GT[CT]^[GA]AC
KpnI           21  GGTACC               GGTAC^C
MaeII        1299  ACGT                 A^CGT
MluNI         434  TGGCCA               TGG^CCA
MscI          434  TGGCCA               TGG^CCA
PflMI        1438  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1636  ATTAAT               AT^TAAT
RcaI          278  TCATGA               T^CATGA
SmaI           14  CCCGGG               CCC^GGG
SmlI         1325  CTCAAG               C^T[CT][GA]AG
SpeI          805  ACTAGT               A^CTAGT
Van91I       1438  CCAGAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          14  CCCGGG               C^CCGGG
XmaI           14  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AocI          991  CCTGAGG              CC^T[ACGT]AGG
             3001  CCTGAGG             
Asp700I       916  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2992  GAAGCCCTTC          
BanII        1712  GGGCTC               G[GA]GC[CT]^C
             2245  GAGCCC              
BglI          148  GCCCCGTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              181  GCCGCCGCGGC         
BmyI         1712  GGGCTC               G[AGT]GC[ACT]^C
             2245  GAGCCC              
BsgI         2918  CTGCAC               GTGCAG(16/14)
              235  GTGCAG              
Bsp1286I     1712  GGGCTC               G[AGT]GC[ACT]^C
             2245  GAGCCC              
BstAPI       1456  GCAATTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2599  GCAGCACCTGC         
BstYI        1155  GGATCT               [GA]^GATC[CT]
             2207  AGATCC              
Bsu36I        991  CCTGAGG              CC^T[ACGT]AGG
             3001  CCTGAGG             
DraII          60  GGGTCCT              [GA]G^G[ACGT]CC[CT]
               86  GGGACCC             
EaeI           27  CGGCCG               [CT]^GGCC[GA]
              434  TGGCCA              
Eco81I        991  CCTGAGG              CC^T[ACGT]AGG
             3001  CCTGAGG             
EcoO109I       60  GGGTCCT              [GA]G^G[ACGT]CC[CT]
               86  GGGACCC             
EcoRI         920  GAATTC               G^AATTC
             1491  GAATTC              
FauI          145  CCCGC                CCCGC(4/6)
             1704  GCGGG               
MfeI         1457  CAATTG               C^AATTG
             2639  CAATTG              
MflI         1155  GGATCT               [GA]^GATC[CT]
             2207  AGATCC              
MslI          168  CACCGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2391  CACTGCAGTG          
MunI         1457  CAATTG               C^AATTG
             2639  CAATTG              
PpuMI          60  GGGTCCT              [GA]G^G[AT]CC[CT]
               86  GGGACCC             
PvuII         296  CAGCTG               CAG^CTG
             2953  CAGCTG              
XhoII        1155  GGATCT               [GA]^GATC[CT]
             2207  AGATCC              
XmnI          916  GAAGGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2992  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaI        AvaII       
BbvI        BcnI        BfaI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com